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Product: RayBio® EIA kits
      Introduction
  Highlight
  How it works
  Contents of kit
  Assay Diagram
  Price
  Service
  Reference List 

Custom 
Peptide Hormone
EIA Kit

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Introduction
The RayBio® Enzyme ImmunoAssay (EIA) is an in vitro quantitative assay for detecting Adipokine peptide based on the principle of Competitive Enzyme Immunoassay. The microplate in the kit is pre-coated with anti-Adipokine polyclonal antibody. After blocking non-specific binding sites, Adipokine antibody could be competitively bound by both biotinylated adipokine peptide and peptide standard or targeted peptide in samples. The biotinylated adipokine peptide then interacts with Streptavidin-horseradish peroxidase (SA-HRP) which catalyzes the substrates. The intensity of chemiluscent signal is directly proportional to the amount of biotinylated peptide-SA-HRP complex and inversely proportional to the amount of adipokine peptide in the standard or samples. This is due to the competitive binding to adipokine antibody between biotinylated Adipokine peptide and peptides in standard or samples. A standard curve of known concentration of adipokine peptide can be established and the concentration of adipokine peptide in the samples can be calculated accordingly.
 
Highlight
High specificity and reproducibility
Adipokines detected at pg/ml and pmol/L levels
Easy to perform and rapid results
Diverse biological samples
Low sample volume required
No additional instrument required
Unique targets; no other assays available
How it works

Contents of kit
96-well Adipokine Microplate (Item A)
Wash Buffer Concentrate (20x) (Item B)
Standard Adipokine Peptide (Item C)
Anti-Nesfatin polyclonal antibody (Item N)
Assay Diluent A (Item D)
Assay Diluent B (Item E)
Biotinylated Nesfatin peptide (Item F)
HRP-Streptavidin concentrate (Item G)
Positive control (Item M)
10.TMB One-Step Substrate Reagent (Item H)
11.Stop Solution (Item I)
13. User Manual (Item K)
 
Representative Results
 
 Price
Product Cat# Format Peptide MW (Dalton) Amino Acid Protein MW (KD) Sensitivity A Sensitivity B Species Sample Type Price
Adiponectin/ Acrp30 EIA-ACP-1 96-well plate 1591.78 244 26.414 pg/ml pM mouse, human, rat cell lysate, supernantant, serum $328
Adipsin EIA-ADP-1 96-well plate - - - - - Human, Mouse, Rat cell lysate, supernantant, serum coming soon
Amylin EIA-AMY-1 96-well plate 3184.5 89 9.806 pg/ml pM mouse, human, rat cell lysate, supernantant, serum $328
Amylin EIA-AMY-1 96-well plate - - - - - Human, Mouse, Rat cell lysate, supernantant, serum coming soon
Angiotensin II EIA-ANGII-1 96-well plate 1598.83 485 53.154 2.62 ng/ml 49.29 pM mouse, human, rat cell lysate, supernantant, serum $328
Apelin EIA-APC-1 96-well plate 1158.33 77 8.569 142 pg/ml 16.57 pM mouse, human, rat cell lysate, supernantant, serum $328
CART EIA-CART-1 96-well plate 1158.43 116 12.829 pg/ml pM mouse, human, rat cell lysate, supernantant, serum $328
Ghrelin EIA-GHR-1 96-well plate 3370.9 117 12.911 161 pg/ml 12.46 pM mouse, human, rat cell lysate, supernantant, serum $328
GLP1  EIA-GLP1-1 96-well plate - - - - - Human, Mouse, Rat cell lysate, supernantant, serum coming soon
Glucagon-like Peptide 2 EIA-GLP-2 96-well plate 3796.22 180 21.115 pg/ml pM mouse, human, rat cell lysate, supernantant, serum $328
Nesfatin EIA-NES-1 96-well plate 1637.84 431 47.5 147 pg/ml 3.09 pM mouse, human, rat cell lysate, supernantant, serum $328
Neuropeptide Y EIA-NPY-1 96-well plate 1993.19 97 10.851 180 pg/ml 16.59 pM mouse, human, rat cell lysate, supernantant, serum $328
Obestatin EIA-OBT-1 96-well plate - - - - - Human, Mouse, Rat cell lysate, supernantant, serum coming soon
Omentin EIA-OME-1 96-well plate - - - - - Human, Mouse, Rat cell lysate, supernantant, serum coming soon
POMC EIA-POMC-1 96-well plate - - - - - Human, Mouse, Rat cell lysate, supernantant, serum coming soon
PYY EIA-PYY-1 96-well plate 4049.55 97 11.046 pg/ml pM mouse, human, rat cell lysate, supernantant, serum $328
RELM beta EIA-RELB-1 96-well plate - - - - - Human, Mouse, Rat cell lysate, supernantant, serum coming soon
Vaspin EIA-VAP-1 96-well plate - - - - - Human, Mouse, Rat cell lysate, supernantant, serum coming soon
Visfatin EIA-VIS-1 96-well plate 3017.35 491 55.521 379 pg/ml 6.82 pM mouse, human, rat cell lysate, supernantant, serum $328
Human Adipokine  Positive Control EIA-PC-01 96-well plate - - - - - Human cell lysate, supernantant, serum coming soon
Adipokine EIA Testing Service EIA-TS-01 96-well plate - - - - - Human, Mouse, Rat cell lysate, supernantant, serum coming soon
Custom EIA assay development EIA-CK-01 96-well plate - - - - - Human, Mouse, Rat cell lysate, supernantant, serum coming soon
 
Reference List
1. Hug C, Lodish HF. Visfatin: a new adipokine. Science. 2005; 307(5708):366-7.
2. Kim MK. Crystal structure of visfatin/pre-B cell colony-enhancing factor 1/nicotinamide phosphoribosyltransferase, free and in complex with the anti-cancer agent FK-866. J Mol Biol. 2006; 362(1):66-77.
3. Revollo, J.R., et al. The NAD biosynthesis pathway mediated by nicotinamide phosphoribosyltransferase regulates Sir2 activity in mammalian cells. J. Biol. Chem. 2004; 279: 50754-50763.
4. Oh-I S, Shimizu H, Satoh T, et al. Identification of nesfatin-1 as a satiety molecule in the hypothalamus". Nature 2006; 443 (7112): 709-12.
5. Zhang J, Ren P, Avsian-Kretchmer O, Luo C, Rauch R, Klein C, Hsueh A. Obestatin, a peptide encoded by the ghrelin gene, opposes ghrelin's effects on food intake. Science 2005; 310 (5750): 996-9.
6. Cummings D, Weigle D, Frayo R, Breen P, Ma M, Dellinger E, Purnell J. Plasma ghrelin levels after diet-induced weight loss or gastric bypass surgery. N Engl J Med 2002; 346 (21): 1623-30.
7. Tschöp M, Smiley DL, Heiman ML. Ghrelin induces adiposity in rodents. Nature 2002; 407 (6806): 908-913.
8. Kojima M, Hosoda H, Date Y, Nakazato M, Matsuo H, Kangawa K. Ghrelin is a growth-hormone-releasing acylated peptide from stomach. Nature 1999; 402 (6762): 656-60.

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