Human RAF1 monoclonal antibody (100 ug)
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Product Description
Specifications
| Size | 100 µg |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Rat |
| Host Species | Mouse |
| Accession Number | AAH18119 |
| Gene Id | 5894 |
| Gene Symbols | RAF1 |
| Protein Name / Synonyms | CRAF, NS5, Raf-1, c-Raf |
| Clonality | Monoclonal |
| Immunogen Sequence | MEHIQGAWKTISNGFGFKDAVFDGSSCISPTIVQQFGYQR RASDDGKLTDPSKTSNTIRVFLPNKQRTVVNVRNGMSLHD CLMKALKVRGLQPECCAVFRLLHEHKGKKARLDWNTDAAS LIGEELQVDF |
| Isotype | Kappa, IgG2b |
| Recommended Applications | Western Blotting, Proximity Ligation Analysis, ELISA |
| Shipping Type | Blue ice |
| Storage | -20°C |
| More Information | Specificity Human |
Synonyms
CRAF, NS5, Raf-1, c-Raf
Description
Mouse monoclonal antibody raised against a partial recombinant RAF1.
Source
Specificity
Human
Gene Information
Gene Accession #
BC018119
Preparation
Storage Buffer
1x PBS, pH 7.2
 | Western Blot detection against Immunogen (39.93 KDa). |
 | RAF1 monoclonal antibody Western Blot analysis of RAF1 expression in rat brain. |
 | Western blot analysis of RAF1 over-expressed 293 cell line, cotransfected with RAF1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with RAF1 monoclonal antibody. GAPDH ( 36.1 kDa ) used as specificity and loading control. |
 | RAF1 monoclonal antibody Western Blot analysis of RAF1 expression in HeLa. |
 | Western Blot analysis of RAF1 expression in transfected 293T cell line by RAF1 monoclonal antibody. Lane 1: RAF1 transfected lysate(73.1 KDa). Lane 2: Non-transfected lysate. |
 | Proximity Ligation Analysis of protein-protein interactions between PDGFRB and RAF1. Huh7 cells were stained with anti-PDGFRB rabbit purified polyclonal 1:600 and anti-RAF1 mouse monoclonal antibody 1:100. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue). |
 | Proximity Ligation Analysis of protein-protein interactions between BAD and RAF1. HeLa cells were stained with anti-BAD rabbit purified polyclonal 1:1200 and anti-RAF1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue). |
Storage
Storage Recommendations
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Expiration:
12 months from the date of shipment when stored properly.