RayBiotech,Inc.

Product: RayBio® Biotin Label-based Antibody Arrays

Introduction

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For the Simultaneous Detection of the Expression Levels of 507 human or 308 Mouse Proteins in Cell Culture Supernates and Serum

Introduction

Recent technological advances by Raybiotech have enabled the largest commercially available antibody array to date. With the L Series Antibody Array, researchers can now obtain a broad, panoramic view of cytokine expression. The expression levels of 507 human or 308 mouse target proteins can be simultaneously detected, including cytokines, chemokines, adipokine, growth factors, angiogenic factors, proteases, soluble receptors, sobuble adhesion molecules , and other proteins in cell culture supernate or serum. Furthermore, an internal control is used to monitor the whole process including biotin-labeling, so this massive array will accurately reflect the available cytokines in your sample. Currently, we have developed a new L-series antibody array for specifically detecting 182 adipokine proteins in a single array.

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	Mutiple Target proteins on a single array !
	Ultra high detection sensitivity
	Direct biotin labeling of proteins
	Utilizes either fluorescence or chemiluminescence
	Accurate and reproducible
	Affordable, quick and simple to use

How it works

Through a simple process, your sample is biotinylated and then dialyzed in preparation for incubation with the array. The process is easy to understand and involves biotinylating the primary amine of the proteins in the sample. From here, the newly biotinylated sample is added onto the antibody arrays either membrane or glass slide and incubated at room temperature. After incubation with HRP-streptavidin or Fluorescent dye-Streptavidin, the signals can be visualized either by chemiluminescence or fluorescence

HRP-streptavidin or Fluorescent dye-Streptavidin

Biotinylated sample

Capture antibody

Contents of kit

	Dialysis Material
	Labeling Reagent
	Internal control
	Stop Solution
	RayBio® Biotin label-based human antibody array I Glass Chip with Frame Blocking Buffer
	20X Wash Buffer I
	20X Wash Buffer II
	Fluorescent dye-Conjugated Streptavidin
	Adhesive film
	Serum Buffer

Assay Diagram

The cell culture supernates or serum is dialyzed before biotin-labeling step. Through a simple process, the primary amine of the proteins in the sample is biotinylated, followed by dialysis to remove free biotin. From here, the newly biotinylated sample is added onto the array membrane or glass slide and incubated at room temperature. After incubation with HRP-streptavidin or Fluorescent Dye-Strepavidin, the signals can be visualized either by chemiluminescence or fluorescence. In this array, we use an internal control to monitoring the whole process including biotin-label and antibody array, it will more exactly respect the levels in your sample. The experimental procedure is simple and can be performed in any laboratory.

Price

Cat#	Description	Size	Detection System	Species	Sample type	Price
AAH-BLG-ADI-1-2	RayBio® Biotin Label-based Human Adipokine Antibody Array 1 (Detect 182 human adipokine proteins)	1 Glass Chip with 2 Subarrays for 2 Samples	Fluorescence (Cy3 channel)	Human	Cell Culture Supernates and Serum	$498.00
AAH-BLG-ADI-1-4	RayBio® Biotin Label-based Human Adipokine Antibody Array 1 (Detects 182 human adipokine proteins)	1 Glass Chip with 4 Subarrays for 4 Samples	Fluorescence (Cy3 channel)	Human	Cell Culture Supernates and Serum	$848.00
AAH-BLG-1-2	RayBio® Biotin Label-based Human Antibody Array 1 (Detect 507 human proteins)	1 Glass Chip with 2 Subarrays for 2 Samples	Fluorescence (Cy3 channel)	Human	Cell Culture Supernates and Serum	$680.00
AAH-BLG-1-4	RayBio® Biotin Label-based Human Antibody Array 1 (Detect 507 human proteins)	2 Glass Chip with 4 Subarrays for 2 Samples	Fluorescence (Cy3 channel)	Human	Cell Culture Supernates and Serum	$1,156.00
AAH-BLM-1-2	RayBio® Biotin Label-based Human Antibody Array 1 (Detect 507 human proteins)	2 membranes for 2 Samples	Chemiluminescence	Human	Cell Culture Supernates	$895
AAH-BLM-1-4	RayBio® Biotin Label-based Human Antibody Array 1 (Detect 507 human proteins)	4 membranes for 4 Samples	Chemiluminescence	Human	Cell Culture Supernates	$1,580
AAM-BLG-1-2	RayBio® Biotin Label-based Mouse Antibody Array 1 (Detect 308 mouse proteins)	1 Glass Chip with 2 Subarrays for 2 Samples	Fluorescence (Cy3 channel)	Mouse	Cell Culture Supernates and Serum	$578.00
AAM-BLG-1-4	RayBio® Biotin Label-based Mouse Antibody Array 1 (Detect 308 mouse proteins)	2 Glass Chip with 4 Subarrays for 2 Samples	Fluorescence (Cy3 channel)	Mouse	Cell Culture Supernates and Serum	$983.00
AAM-BLM-1-2	RayBio® Biotin Label-based Mouse Antibody Array 1 (Detect 308 mouse proteins)	2 array membranes for 2 Samples	Chemiluminescence	Mouse	Cell Culture Supernates	$778
AAM-BLM-1-4	RayBio® Biotin Label-based Mouse Antibody Array 1 (Detect 308 mouse proteins)	4 array membranes for 4 Samples	Chemiluminescence	Mouse	Cell Culture Supernates	$1,368
AAR-BLG-1-4	RayBio® Biotin Label-based Rat Antibody Array 1 (Detect 90 rat proteins)	1 Glass Chip with 4 Subarrays for 4 Samples	Fluorescence (Cy3 channel)	Rat	Cell Culture Supernates and Serum	$790.00
AAR-BLG-1-8	RayBio® Biotin Label-based Rat Antibody Array 1 (Detect 90 rat proteins)	2 Glass Chip with 8 Subarrays for 8 Samples	Fluorescence (Cy3 channel)	Rat	Cell Culture Supernates and Serum	$1,480.00
AAR-BLM-1-2	RayBio® Biotin Label-based Rat Antibody Array 1 (Detect 90 rat proteins)	2 array membranes for 2 Samples	Chemiluminescence	Rat	Cell Culture Supernates	$498
AAR-BLM-1-4	RayBio® Biotin Label-based Rat Antibody Array 1 (Detect 90 rat proteins)	4 array membranes for 4 Samples	Chemiluminescence	Rat	Cell Culture Supernates	$888

Service

Cat#	Description	Application	Detection System	Species	Price / per sample
AAH-BLG-ADI-1-Serv-2	measuring 2-3 samples using glass chip	Cell Culture Supernates and Serum	Fluorescence (Cy3)	Human	$498
AAH-BLG-ADI-1-Serv-4	measuring 4-7 samples using glass chip	Cell Culture Supernates and Serum	Fluorescence (Cy3)	Human	$418
AAH-BLG-ADI-1-Serv-8	measuring 8-20 samples using glass chip	Cell Culture Supernates and Serum	Fluorescence (Cy3)	Human	$378
AAH-BLG-ADI-1-Serv-20	measuring over 20 samples using glass chip	Cell Culture Supernates and Serum	Fluorescence (Cy3)	Human	$328
AAH-BLG-Serv-2	measuring 2-3 samples using glass chip	Cell Culture Supernates and Serum	Fluorescence (Cy3)	Human	$685
AAH-BLG-Serv-4	measuring 4-7 samples using glass chip	Cell Culture Supernates and Serum	Fluorescence (Cy3)	Human	$520
AAH-BLG-Serv-8	measuring 8-20 samples using glass chip	Cell Culture Supernates and Serum	Fluorescence (Cy3)	Human	$480
AAH-BLG-Serv-20	measuring over 20 samples using glass chip	Cell Culture Supernates and Serum	Fluorescence (Cy3)	Human	$420
AAH-BLM-Serv-2	measuring 2-3 samples using membrane array	Cell Culture Supernates	Chemiluminescence	Human	$815
AAH-BLM-Serv-4	measuring 4-7 samples using membrane array	Cell Culture Supernates	Chemiluminescence	Human	$695
AAH-BLM-Serv-8	measuring 8-20 samples using membrane array	Cell Culture Supernates	Chemiluminescence	Human	$620
AAH-BLM-Serv-20	measuring over 20 samples using membrane array	Cell Culture Supernates	Chemiluminescence	Human	$580
AAM-BLG-Serv-2	measuring 2-3 samples using glass chip	Cell Culture Supernates and Serum	Fluorescence (Cy3)	Mouse	$582
AAM-BLG-Serv-4	measuring 4-7 samples using glass chip	Cell Culture Supernates and Serum	Fluorescence (Cy3)	Mouse	$442
AAM-BLG-Serv-8	measuring 8-20 samples using glass chip	Cell Culture Supernates and Serum	Fluorescence (Cy3)	Mouse	$408
AAM-BLG-Serv-20	measuring over 20 samples using glass chip	Cell Culture Supernates and Serum	Fluorescence (Cy3)	Mouse	$357
AAM-BLG-Serv-2	measuring 2-3 samples using glass chip	Cell Culture Supernates	Chemiluminescence	Mouse	$745
AAM-BLG-Serv-4	measuring 4-7 samples using glass chip	Cell Culture Supernates	Chemiluminescence	Mouse	$630
AAM-BLG-Serv-8	measuring 8-20 samples using glass chip	Cell Culture Supernates	Chemiluminescence	Mouse	$560
AAM-BLG-Serv-20	measuring over 20 samples using glass chip	Cell Culture Supernates	Chemiluminescence	Mouse	$520
AAR-BLG-Serv-2	measuring 2-3 samples using glass chip	Cell Culture Supernates and Serum	Fluorescence (Cy3)	Rat	$523
AAR-BLG-Serv-4	measuring 4-7 samples using glass chip	Cell Culture Supernates and Serum	Fluorescence (Cy3)	Rat	$397
AAR-BLG-Serv-8	measuring 8-20 samples using glass chip	Cell Culture Supernates and Serum	Fluorescence (Cy3)	Rat	$367
AAR-BLG-Serv-20	measuring over 20 samples using glass chip	Cell Culture Supernates and Serum	Fluorescence (Cy3)	Rat	$321
AAR-BLM-Serv-2	measuring 2-3 samples using glass chip	Cell Culture Supernates and Serum	Fluorescence (Cy3)	Rat	$598
AAR-BLM-Serv-4	measuring 4-7 samples using glass chip	Cell Culture Supernates and Serum	Fluorescence (Cy3)	Rat	$498
AAR-BLM-Serv-8	measuring 8-20 samples using glass chip	Cell Culture Supernates and Serum	Fluorescence (Cy3)	Rat	$448
AAR-BLM-Serv-20	measuring over 20 samples using glass chip	Cell Culture Supernates and Serum	Fluorescence (Cy3)	Rat	$398

Analysis Software

AAH-BLG-ADI-1-SW	Analysis software L series Human Adipokine 182 (membrane or slide)	$160
AAH-BLG-1-SW	Analysis software L series Human 507 (slide)	$220
AAH-BLM-1-SW	Analysis software L series Human 507 (membrane)	$220
AAM-BLG-SW	Analysis software L series Mouse 308 (slide)	$200
AAM-BLM-SW	Analysis software L series Mouse 308 (membrane)	$200
AAR-BLG-SW	Analysis software L series rat (slide)	$180
AAR-BLM-SW	Analysis software L series rat (membrane)	$180

Examples of Array

A) Human L-Series 507

The figure shows the RayBio® Biotin-label-based Human Antibody Array I probed with two cell culture supernates. The image were captured using a Axon GenePix laser scanner.

B) Mouse L-Series 308

The figure shows the RayBio® Biotin-label-based Mouse Array 1 probed with Mouse sample. The image were captured using a Axon GenePix laser scanner.

Reference List

1. Lin Y, Huang R, Chen L, et al. Profiling of cytokine expression by biotin-labeled-based protein arrays. Proteomics. 2003, 3: 1750-1757.

2. Ksendzovsky A, Feinstein D, Zengou R, Sharp A, Polak P, et al. Investigation of immunosuppressive mechanisms in a mouse glioma model. Journal of Neuro-Oncology. 2009, 93(1):107–114.

3. Tang YL, Zhu E. Cheng M, Chen L, Zhang J, et al. Hypoxic Preconditioning Enhances the Benefit of Cardiac Progenitory Cell Therapy for Treatment of Myocardial Infarction by Inducing CXC3 Expression. Circulation Research. 2009;104

4. Huang R, Yang Q, Huang R. Identification of new biomarkers in ovarian cancer using biotin-label-based protein antibody arrays. AACR Meeting Abstracts. April 2008; 2008:5170.

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