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Product: RayBio® Biotin Label-based
Antibody Arrays |
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| For the Simultaneous Detection of the Expression
Levels of 507 human or 308 Mouse Proteins in Cell Culture Supernates
and Serum |
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| Recent technological advances by Raybiotech
have enabled the largest commercially available antibody array
to date. With the L Series Antibody Array, researchers can now
obtain a broad, panoramic view of cytokine expression. The expression
levels of 507 human or 308 mouse target proteins can be simultaneously
detected, including cytokines, chemokines, adipokine, growth
factors, angiogenic factors, proteases, soluble receptors, sobuble
adhesion molecules , and other proteins in cell culture supernate
or serum. Furthermore, an internal control is used to monitor
the whole process including biotin-labeling, so this massive
array will accurately reflect the available cytokines in your
sample. Currently, we have developed a new L-series antibody
array for specifically detecting 182 adipokine proteins in a
single array. |
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Mutiple
Target proteins on a single array ! |
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Ultra high detection
sensitivity |
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Direct biotin labeling
of proteins |
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Utilizes either fluorescence
or chemiluminescence |
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Accurate and reproducible |
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Affordable, quick and
simple to use |
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| Through a simple process,
your sample is biotinylated and then dialyzed in preparation
for incubation with the array. The process is easy to understand
and involves biotinylating the primary amine of the proteins
in the sample. From here, the newly biotinylated sample is added
onto the antibody arrays either membrane or glass slide and
incubated at room temperature. After incubation with HRP-streptavidin
or Fluorescent dye-Streptavidin, the signals can be visualized
either by chemiluminescence or fluorescence |
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| HRP-streptavidin
or Fluorescent dye-Streptavidin |
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| Biotinylated
sample |
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| Capture antibody |
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Dialysis Material |
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Labeling Reagent |
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Internal control |
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Stop Solution |
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RayBio® Biotin label-based
human antibody array I Glass Chip with Frame Blocking
Buffer |
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20X Wash Buffer I |
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20X Wash Buffer II
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Fluorescent dye-Conjugated
Streptavidin |
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Adhesive film |
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Serum Buffer |
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| 1. Profiling of cytokine expression
by biotin-labeled-based proteinarrays. Ying Lin, Ruochun
Huang, Lipai Chen et al. Proteomics.2003, 3: 1750-1757. |
| 2. Proteomic profiling of the cancer
microenvironment by antibody arrays. Vladimir Knezevic,
Chidchanok Leethanakul, Verena E. Bichsel et al. Proteomics
2001, 1, 1271–1278 |
| 3. Antibody microarray profiling
of human prostate cancer sera: Antibody screening and
identification of potential biomarkersJeremy C. Miller,
Heping Zhou, Joshua Kwekel, Robert Cavalloet al. The,
Brian B. Haab. Proteomics. 2003, 3: 56-63. |
| 4. Smad4 signalling in T cells is
required for suppression of gastrointestinal cancer.
Byung-Gyu Kim, Cuiling Li, WenhuiQiao, Mizuko Mamura,
Barbara Kasperczak, Miriam Anver, Lawrence Wolfraim,
Suntaek Hong, Elizabeth Mushinski, Michael Potter, Seong-Jin
Kim, Xin-Yuan Fu, Chuxia Deng and John J. Letterio.
Nature. 2006; Vol 441: 1015 |
| 5. Connexin suppresses human glioblastoma
cell growth by down-regulation of monocyte chemotactic
protein 1, as discoveredusing protein array technology.
Ruochun Huang, Ying Lin, Cheng C. Wang, Jacob Gano,
Biaoyang Lin, Qian Shi, AltonBoynton, Jocelyn Burke,
and Ruo-Pan Huang. Cancer Res. 2002;62:2806-2812. |
| 6. LPS induces the interaction of
a transcription factor, LPS-induced TNF-a factor, and
STAT6(B) with effects on multiple cytokines.Xiaoren
Tang, Deborah Levy Marciano, Susan E. Leeman and Salomon
Amar. PNAS. April 5, 2005 vol. 102 no. 14, 5132-5137
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| 7. HIV-1-mediated apoptosis of neuronal
cells: Proximal molecular mechanisms of HIV-1-induced
encephalopathy. Yan Xu, JosephKulkoshy, Roger j. Pomerantz.
PNAS. 2004 May 4, 2004 Vol. 101 No. 18. |
| 8. A novel method for high-throughput
protein profiling fromconditioned media and patient’s
sera. Ruo-Pan Huang, RuochunHuang, Yan Fan, and Ying
Lin. Ana. Biochem. 2001;294(1):55-62. |
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