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Allergen Protein Arrays

Introduction

The RayBio® Allergen Protein Arrays are semi-quantitative multiplex protein immunoassays developed for the detection of common allergens. Recombinant allergen proteins or extracted native allergen proteins are spotted onto a solid glass slide surface, and then probed for the presence of serum specific antibodies to these allergen targets. These detection kits can then be applied in screening specific allergens based on IgE and/or IgG levels present in patient serum samples.

For a comparison & overview of all our protein array systems, click here.

 

View Product Page    Compare Protein Array Target Lists    

 
 

Allergen Protein Array Features

Multichannel PipetteGet 3x the data, plus high throughput

Each 75mm x 25mm glass slide is spotted with either 4 or 16 identical protein arrays (also called  “subarrays”). Within each subarray, the allergens along with controls are spotted in triplicate. The slide comes with a 16-well removable gasket and chamber assembly that allows for simultaneous processing of up to 16 samples per slide (4 if using the 4 sample kit). To achieve even higher throughput, 4 slides can be nested into a tray which matches a standard microplate, allowing for automated processing of 64 arrays (If using the 16 sample kit) simultaneously. The latest automated liquid handling work stations can accommodate several trays at once, allowing hundreds of samples to be processed per day. 

Features

 

Contents of kit

  • Less sample, more data: only 25 ul of sample is needed for quantification of up to 48 common allergens
  • Les than 8-hour processing time
  • No interference between immobilized proteins (unlike bead-based multiplex assays)
  • No dedicated equipment required
  • Customizable – create a custom array from our list of targets
 
    • Glass Chip with Protein Arrays
    • Biotin-conjugated Anti-human IgG
    • Biotin-conjugated Anti-human IgE
    • Blocking Buffer
    • Streptavidin-Fluorescent dye
    • Wash buffers
    • Manual

 *Accessories include: 16-well incubation chamber with gasket, protective cover, snap-on sides, adhesive film

  

How It Works

Allergen Protein Array - How it works

 

A panel of allergen proteins is printed in multiple identical arrays on a standard slide. After a blocking step, samples are incubated with the arrays. Nonspecific antibodies are then washed off, and the arrays are incubated with Biotin-conjugated anti-human IgG and/or IgE detection antibodies, followed by a streptavidin-conjugated fluor. Signals are then visualized using a fluorescence laser scanner.

 

 

 

 

 

For a list of laser scanner specifications, click here.

Testing Service

Full Testing Service: Ship us your samples (5 sample minimum) and our experienced service department will run the array and analyze the data for you. Email our tech support team at techsupport@raybiotech.com to learn more.

  

Scanning Service: If you do not have access to a scanner, RayBiotech provides slide scanning and data analysis services

RayBiotech Protein Array slide scanning and data extraction service is now FREE for a limited time only! Simply ship us your slide/s and we will scan the slide/s and send you the raw data extracted from the image. Click to learn more

Need help analyzing all that data? Copy and paste your data into the RayBiotech Analysis Tool specific for your array. The analysis will automatically perform background subtraction and normalization.

Research Applications

          • High-throughput profiling of allergens in human serum
          • Identifying potential allergens based on IgE and IgG levels
 

Human Allergen Protein Array G1

Contains 48 common allergen proteins:

No.

Allergen Name

Species - Scientific Name

1 Act c 1 Kiwi - Actinidia chinensis
2 Act c 2 Kiwi - Actinidia chinensis
3 Alt a 1 Alternaria rot fungus - Alternaria alternata
4 Ana c 2 Pineapple - Ananas comosus
5 Ara h 1 Peanut - Arachis hypogaea
6 Ara h 2 Peanut - Arachis hypogaea
7 Ara h 3 Peanut - Arachis hypogaea
8 Ara h 6 Peanut - Arachis hypogaea
9 Art v 3 Mugwort - Artemisia vulgaris
10 Asp f 1 Aspergillus fumigatus
11 Bla g 5 German cockroach - Blattella germanica
12 Bla g 6 German cockroach - Blattella germanica
13 Bos d 6 Cow (bovine) - Bos taurus
14 Can f 1 Dog (canine) - Canis familiaris
15 Cas s 8 Sweet Chestnut - Castanea sativa
16 Che a 2 Lamb's-quarters - Chenopodium album
17 Che a 3 Lamb's-quarters - Chenopodium album
18 Cla h 5 Mycosphaerella tassiana - Davidiella tassiana (Cladosporium herbarum)
19 Cla h 8 Mycosphaerella tassiana - Davidiella tassiana (Cladosporium herbarum)
20 Cor a 14 European hazelnut - Corylus avellana
21 Cor a 9 European hazelnut - Corylus avellana
22 Cte f 1 Cat flea - Ctenocephalides felis
23 Cuc m 2 Muskmelon - Cucumis melo
24 Der f 2 American house dust mite - Dermatophagoides farinae
25 Der p 1 European house dust mite - Dermatophagoides pteronyssinus
26 Der p 2 European house dust mite - Dermatophagoides pteronyssinus
27 Fel d 1 Cat (feline) - Felis catus
28 Fus c 1 Fusarium culmorum
29 Gly d 2 House itch mite - Glycyphagus domesticus
30 Jug r 1 English walnut - Juglans regia
31 Jug r 2 English walnut - Juglans regia
32 Jug r 3 English walnut - Juglans regia
33 Jug r 4 English walnut - Juglans regia
34 Lep d 2 Fodder mite - Lepidoglyphus destructor
35 Mal d 2 Apple - Malus domestica
36 Mal d 3 Apple - Malus domestica
37 Mus a 4 Banana - Musa acuminata
38 Mus m 1 Mouse - Mus musculus
39 Pen c 3 Penicillium citrinum
40 Pen n 13 Penicillium notatum - Penicillium chrysogenum
41 Per a 1 American cockroach - Periplaneta americana
42 Phl p 1 Common timothy - Phleum pratense
43 Phl p 12 Common timothy - Phleum pratense
44 Pho d 2 Date palm - Phoenix dactylifera
45 Pru p 2.2 Prunus dulcis x Prunus persica
46 Pru p 3 Peach - Prunus persica
47 Rat n 1 Rat - Rattus norvegicus
48 Tri a 14 Wheat - Triticum aestivum

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Human Allergen Protein Array G2

Contains 22 common allergen proteins:

No.

Allergen Name

Species - Scientific Name

1 Alt a 1 Alternaria rot fungus - Alternaria alternata
2 Asp f 1 Aspergillus fumigatus
3 Bla g 5 German cockroach - Blattella germanica
4 Bla g 6 German cockroach - Blattella germanica
5 Can f 1 Dog - Canis lupus familiaris
6 Che a 2 Lamb's-quarters, pigweed - Chenopodium album
7 Che a 3 Lamb's-quarters, pigweed - Chenopodium album
8 Cla h 5 Cladosporium herbarum mold
9 Cla h 8 Cladosporium herbarum mold
10 Cte f 1 Cat flea - Ctenocephalides felis
11 Der f 2 American house dust mite - Dermatophagoides farinae
12 Der p 1 European house dust mite - Dermatophagoides pteronyssinus
13 Der p 2 European house dust mite - Dermatophagoides pteronyssinus
14 Fel d 1 Cat (saliva) - Felis catus
15 Fus c 1 Fusarium culmorum
16 Gly d 2 House itch mite - Glycyphagus domesticus
17 Lep d 2 Storage mite - Lepidoglyphus destructor
18 Mus m 1 Mouse - Mus musculus
19 Pen c 3 Penicillium citrinum
20 Pen n 13 Penicillium chrysogenum
21 Per a 1 American cockroach - Periplaneta americana
22 Rat n 1 Rat (urine) - Rattus norvegicus

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