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Products - Custom Antibody Service

Can't find a good antibody to your target molecule?
We can generate almost any kind of antibody, including recombinant and engineered.

Polyclonal Antibody

Monoclonal Antibody

Recombinant Antibody

Phage Display

Antibody Engineering

Antibody Purification and Conjugation

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Outline of Antibody Generation Service

Service Items	Time	Description	Price
Part 1. Polyclonal Antibody	5-10 Weeks	Recognizing multiple antigenic sites of injected immunogen, immunization, high ELISA titer, affinity purity	$800
Part2. Monoclonal Antibody	4-5 months	recognizing one antigenic binding site of the antigen, Immunization, fusion, screening, subclone, characterization, affinity purity	$4,200
Part 3. Recombinant Antibody	6 weeks	recognizing one or two antigenic binding site of the antigen, Peptide Design and synthesis, selecting, screening, characterization, affinity purity, sequence and analysis of antibody gene	Inquire
Part 4. Phage Display	6-8 weeks	Creating antibody binding to any chosen target antigen without the use of laboratory animals or hybridomas	Inquire
Part5. Antibody Engineering	Variable	isolation of genes encoding the specific antibody, creating the ability to redesign, enhance or alter specific antibody function based on antibody gene	Inquire
Part 6. Antibody Purification and Conjugation	2-3 days	conjugating polyclonal, monoclonal and recombinant antibodies, with Enzymes, fluorochromes, affinity ligand and solid surface conjugation	Inquire

Part 1. Polyclonal Antibody

Service items	Time	Criteria	Price
Anti-regular peptide antibody	5 weeks	1. 4 mg of peptide from customer	$800
		2. Immunization of two rabbits
		3. 100 ml of immune sera
		4. Generated antibody recognizes immunogen in ELISA assay
		5. ELISA titer is up to 1:10000
		6. Antibody purification by affinity column may be obtained upon request

Anti-regular peptide antibody	8 weeks	1. Design, synthesize and conjugate peptide(15-20mer,5-10mg)	$1,400
		2. Immunization of two rabbits
		3. 100 ml of immune sera
		4. Generated antibody recognizes immunogen in ELISA assay
		5. ELISA titer is up to 1:10000
		6. Antibody purification by affinity column may be obtained upon request

Anti-phospho-peptide antibody	8-10 weeks	1. Design, synthesize, specific site phophorylation and conjugate peptide(12-15mer,5-10mg)	$2,500
		2. Immunization of two rabbits
		3. 100 ml of immune sera
		4. Generated antibody recognizes immunogen in ELISA assay
		5. ELISA titer is 1:1000
		6. Antibody purification by affinity column may be obtained upon request

Anti-protein antibody	5-7 weeks	1. 5 mg of purified Protein from customer	$800
		2. Immunization of two rabbits
		3. 100 ml of immune sera
		4. Generated antibody recognizes immunogen in ELISA assay
		5. ELISA titer is up to 1:8000
		6. Antibody purification by affinity column may be obtained upon request

Part 2. Monoclonal Antibody

Service Items	Time	Criteria	Price
Anti-regular peptide antibody (customer provide antigen)	4-5 months	1. Customer provide peptide(4mg)	$4,200
		2. Immunization of two mice, fusion, screening, subclone and initial characterization
		3. With up to three monoclones and their supernatant for confirming detection
		4. Generated antibody recognizes immunogen in ELISA assay
		5. Purify Antibody from 100 ml of hybridoma culture supernatant or 1 ml of ascites fluid


Anti-regular peptide antibody (RayBiotech provide antigen)	4-5 months	1. Design, synthesize and conjugate peptide(15-20mer,5-10mg)	$4,800
		2. Immunization of two mice, fusion, screening, subclone and initial characterization
		3. With up to three monoclones and their supernatant for confirming detection
		4. Generated antibody recognizes immunogen in ELISA assay
		5. Purify Antibody from 100 ml of hybridoma culture supernatant or 1 ml of ascites fluid


Anti-phospho-peptide antibody	5-6 months	1. Design, synthesize, specific site phophorylation and conjugate peptide(11-15mer, 5 mg)	$5,800
		2. Immunization of two mice, fusion, screening, subclone and initial characterization
		3. With up to three monoclones and their supernatant for confirming detection
		4. Generated antibody recognizes immunogen in ELISA assay
		5. Antibody purification by affinity column(1-5mg) may be obtained upon request


Anti-protein antibody	5 months	1. 4mg of purified Protein from customer	$4,200
		2. Immunize two mice, fusion, screening, subclone and initial characterization
		3. with up to three monoclones and their supernatant for confirming detection
		4. Generated antibody recognizes immunogen in ELISA assay
		5. Antibody purification by affinity column(1-5mg) may be obtained upon request

Part 3. Recombinant antibody

Why Use Recombinant Antibody:

Generating antibody without using animal
Unstable hybridoma lines or primary fusion cultures can be saved by rescuing and cloning the antibody gene.
The size and specificity of Recombinant antibody are smaller and higher than traditional antibody(polyclonal or monoclonal), so that it may allow for attachment or penetration to cell or target sites.
Monoclonal isotype switching and affinity optimization using recombinant DNA techniques
The purified antibody gene can be mutated to effect binding specificity or neutralizing capabilities.
Increase assay sensitivity through antibody manipulation.
Create multiple binding antibody
Create antibody-fusion protein or peptide
Unlimited supply of antibody through bacterial culture.
Removes the expense and issues associated with ascites production in mice or serum from animals.
Allow to generate antibody to all targets, even small molecules.

Service items	Time	Criteria	Price
Anti-regular peptide antibody	6 weeks	1. Design, synthesize and biotinylated peptide(15-20mer,5-10mg)	Please Inquire
		2.Selecting, screening, and initial characterization
		3. with up to five ELISA positive clones
		4. 10 ml of Phage and soluble antibody for customer confirming detection
		5. Antibody gene sequence and analysis for specific clone
		6. Antibody purification by affinity column may be obtained upon request
		7. Customer clone ownership agreement

Anti-protein antibody	6 weeks	1. 5mg of purified Protein from customer and biotinylated it	Please Inquire
		2. Selecting, screening, and initial characterization
		3. with up to five ELISA positive clones
		4. 10 ml of Phage and soluble antibody for customer confirming detection
		5. Antibody gene sequence and analysis for specific clone
		6. Antibody purification by affinity column may be obtained upon request
		7. Customer clone ownership agreement

Single chain antibody from hybridoma	2-3 months	1. Establish specific immunoassay based on customer’s requirement(fixing amount of antigen)	Please Inquire
		2. Grow hybridoma in-house and evaluate antibody secretion ability
		3. Purify mRNA and synthesize cDNA
		4. Amplify specific antibody gene by PCR, assemble and cloning them into expressing vector
		5. Selecting, screening, and initial characterization of ScFv format antibody derived from parent hybridoma
		6. With up to five ELISA positive clones
		7. 10 ml of Phage and soluble antibody for customer confirming detection
		8. Antibody gene sequence and analysis of specific characterized clone
		9. Antibody purification by affinity column may be obtained upon request
		10. Customer clone ownership agreement

Single chain antibody from immunized mouse		1. 5mg of antigen from customer	Please Inquire
		2. Immunize two mice,
		3. Collect immune sera and establish specific immunoassay
		4. Collect spleen and blood B cell, purify mRNA and synthesize cDNA
		5. Amplify specific antibody gene by PCR, assemble and cloning them into expressing vector
		6. Construct specific-antigen antibody library
		7. Selecting, screening, and initial characterization of ScFv format antibody from the displayed library
		8. With up to five ELISA positive clones
		9. 10 ml of Phage and soluble antibody for customer detection
		10. Antibody gene sequence and analysis for specific clone
		11. Antibody purification by affinity column


Single chain antibody based on available gene	1. 1 mg of antigen and 10 µg of purified DNA from customer	Please Inquire
	2. Design specific primers and amplify specific antibody gene by PCR
	3. Assemble and cloning them into expressing vector
	4. Selecting, screening, and initial characterization of ScFv format antibody
	5. With ELISA positive clones
	6. 10 ml of Phage and soluble antibody for customer detection
	7. Antibody gene sequence and analysis for specific clone
	8. Antibody purification by affinity column

Single chain antibody based on synthetic DNA	1. Design and synthesize oligonucleotides based on customer requirement	Please Inquire
	2. Establish specific immunoassay with antigen from customer
	3. Ligate oligonucleotides, amplify DNA by PCR, assemble and cloning them into expressing vector
	4. Selecting, screening, and initial characterization of ScFv format antibody from the expressing clones
	5. With ELISA positive clones
	6. Verify cloning antibody gene by sequence
	7. 10 ml of Phage and soluble antibody for customer detection
	8. Antibody gene sequence and analysis for specific clone
	9. Antibody purification by affinity column

Antibody humanization	1. Antigen, antibody, parent clones with murine antibody gene, clear sequence and suggesting immunoassay from customer	Please Inquire
	2. Confirm antibody binding activity with establishing in-house specific immunoassay
	3. Sequence comprehensive analysis by our tools
	4. Humanization working strategy and plan
	5. Design and synthesize a set of primers
	6. Specific sites mutation, antibody activity and sequence confirm after each cycle mutation.
	7. First hand of humanization modified plan.
	8. Selecting, screening, and extensive characterization of humanized ScFv format antibody from the mutated library
	9. Desired clones further evaluation
	10. Antibody purification by affinity column

Part 4. Antibody Phage Display

Phage display provides a direct physical link between phenotype and genotype by utilizing the ability to display genes on the surface of bacteriophage. Why use phage display to make recombinant antibody? Traditional ways for preparing antibodies are cumbersome, labor intensive, amount limited and unstable of antibody and animal use. It is now possible to create antibodies binding to any chosen target antigen without the use of laboratory animals or hybridomas, in a system that completely by-passes the immune system. Therefore, antibodies can be displayed on phage in form of single chain antibody (scFv) or Fab' fragments using the system. It allows us to quickly construct different format of antibody libraries. It gives new possibilities for providing novel binders to almost any antigen, including those that are poorly immunogenic or non-immunogenic, self-antigen or 'difficult' targets.

Selection and screening of phage-displayed antibody

We practically provide all antibody displayed service as follows:
1. Further modification of well-characterized antibody or antigen by phage display.
2. Creation of target-based mutated antibody phage display library
3. Quick selection and screening of Single chain antibody from antibody library

Part 5. Antibody Engineering

In antibody engineering, molecular biology approaches are used to improve the function of antibody molecules by altering their amino acid sequence or fusion them to other functional peptides or proteins. The affinity and specificity with which antibodies recognize antigens, their stability in various environmental conditions, their therapeutic efficacy, and their detection in diagnostic applications may thus be enhanced. The platform provides opportunity for the isolation of genes encoding the specific antibody, which therefore creates the ability to redesign, enhance or alter specific antibody function.

Followings are suitable for modified your antibody:

Well characterized non-human antibody
Unique monoclonal antibody but it is produced from unstable hybridoma
Want to change your antibody format
Improve antibody functions
Convert scFv to whole IgG

We also offer customized antibody engineering including techniques of antibody sequence cloning, converting monoclonal antibody into single chain antibody, humanization and generation of antibody constructs, such as creation of multivalent, multi-specific or multifunctional antibody derivatives or fusion-antibody.

Part 6. Antibody Purification and Conjugation

All format of antibodies can be purified by affinity column before labeling. We provide conjugating polyclonal, monoclonal and recombinant antibodies, with the following labels:

Enzymes, including alkaline phosphatase and horseradish peroxidase, etc.
Fluorochromes: FITC and Cyanine dyes (Cy3, Cy5 and Cy7 ), etc.
Affinity ligand conjugation: Biotin, streptavidin, protein A, etc.
Solid surface: Quantum Dot, Agarose beads, magnetic beads, etc.
Others available upon request

Our antibody conjugates are used for a different applications such as flow cytometry, ELISA, Western blotting, immunohistochemistry and membrane assays.