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Rat Cytokine Array C1

CODE: AAR-CYT-1-2

$283.00

RayBio® C-Series Rat Cytokine Antibody Array 1 Kit. Detects 19 Rat Cytokines. Suitable for all liquid sample types.


Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information

CINC-2
CINC-3
CNTF
Fractalkine
(CX3CL1)
GM-CSF
IFN-gamma
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-4
IL-6
IL-10
LIX
Leptin
MCP-1
(CCL2)
MIP-3 alpha
(CCL20)
beta-NGF
TIMP-1
TNF alpha
VEGF-A

Species Detected

Rat

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Rat Cytokine Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Number of Targets Detected

19

Product Specifications

  • 4 or 8 Sample Kit

Array/ELISA Format

  • Rat
  • Membrane
  • Sandwich-based
  • Semi-Quantitative
  • Chemiluminescence
  • Serum
  • Plasma
  • Cell Culture Supernatants
  • Cell Lysates
  • Tissue Lysates

  1. Shi S., Liang D., Bao M., et al. Gx-50 Inhibits Neuroinflammation via α7 nAChR Activation of the JAK2/STAT3 and PI3K/AKT Pathways. J Alzheimers Dis. 2016 Jan 21. Epub ahead of print
    Species: Mouse
    Sample Type: Conditioned Media

  2. Tsai T., Lee T., Liu M., et al. Release of endogenous heat shock protein 72 on the survival of sepsis in rats. J Surg Res. 2015 Sep;198(1):165-74. doi: 10.1016/j.jss.2015.05.004.
    Species: Rat
    Sample Type: Serum

  3. Sala R., Mena P., Savi M., et al. Urolithins at physiological concentrations affect the levels of pro-inflammatory cytokines and growth factor in cultured cardiac cells in hyperglucidic conditions. Journal of Functional Foods Volume 15, May 2015, Pages 97-105. doi:10.1016/j.jff.2015.03.019
    Species: Rat
    Sample Type: Conditioned Media

  4. Gu P., Qiu F., Han R., et al. Efficient differentiation of neural stem cells induced by the rat bone marrow stromal cells. Int J Clin Exp Med. 2015 May 15;8(5):6713-24.
    Species: Rat
    Sample Type: Conditioned Media

  5. Deuse T., Hua X., Wang D., Maegdefessel L., et al. Dichloroacetate prevents restenosis in preclinical animal models of vessel injury. Nature (2014) doi:10.1038/nature13232 [Epub ahead of print]
    Species: Rat
    Sample Type: Tissue Lysate

  6. Yang D., Sun YY., Bhaumik SK., et al. Blocking lymphocyte trafficking with FTY720 prevents inflammation-sensitized hypoxic-ischemic brain injury in newborns. J Neurosci. 2014 Dec 3;34(49):16467-81. doi: 10.1523/JNEUROSCI.2582-14.2014
    Species: Rat
    Sample Type: Tissue Lysate

  7. Busch-Dienstfertig M., Labuz D., Wolfram T., et al. JAK-STAT1/3-induced expression of signal sequence-encoding proopiomelanocortin mRNA in lymphocytes reduces inflammatory pain in rats. Mol Pain. 2012 Nov 13;8:83. doi: 10.1186/1744-8069-8-83
    Species: Rat
    Sample Type: Tissue Lysate

  8. Merabova N, Kaminski R, Krynska B, Amini S, Khalili K, Darbinyan A. JCV agnoprotein-induced reduction in CXCL5/LIX secretion by oligodendrocytes is associated with activation of apoptotic signaling in neurons. Journal of Cellular Physiology. 2012;227(8):3119-3127. doi:10.1002/jcp.23065.
    Species: Rat
    Sample Type: Conditioned Media

  9. Schaal SM, Garg MS, Ghosh M, Lovera L, Lopez M, et al. (2012) The Therapeutic Profile of Rolipram, PDE Target and Mechanism of Action as aNeuroprotectant following Spinal Cord Injury. PLoS ONE 7(9): e43634. doi:10.1371/journal.pone.0043634
    Species: Rat
    Sample Type: Tissue Lysate

  10. Liu J., Xu C., Chen L., et al. Involvement of Kv1.3 and p38 MAPK signaling in HIV-1 glycoprotein 120-induced microglia neurotoxicity. Cell Death Dis. 2012 Jan 19;3:e254. doi: 10.1038/cddis.2011.140.
    Species: Ray
    Sample Type: Conditioned Media

  11. Ogawa T., de Bold AJ. Uncoordinated regulation of atrial natriuretic factor and brain natriuretic peptide in lipopolysaccharide-treated rats. Biomarkers. 2012 Mar;17(2):140-9. doi: 10.3109/1354750X.2011.643487.
    Species: Rat
    Sample Type: Plasma

  12. Holubova M., Leva M., Sedmikova M., et al. Characterization of three newly established rat sarcoma cell clones. In Vitro Cell Dev Biol Anim. 2012 Dec;48(10):610-8. doi: 10.1007/s11626-012-9563-3.
    Species: Rat
    Sample Type: Conditioned Media

  13. Chen M., Cheung F., Chan M., et al. Protective rolesofCordycepsonlungfibrosisincellularandratmodels. J Ethnopharmacol. 2012 Sep 28;143(2):448-54. doi: 10.1016/j.jep.2012.06.033
    Species: Rat
    Sample Type: Tissue Lysate

  14. Mazo M., Cemborain A., Gavira J., et al. Adipose stromal vascular fraction improves cardiac function in chronic myocardial infarction through differentiation and paracrine activity. Cell Transplant. 2012;21(5):1023-37. doi: 10.3727/096368911X623862
    Species: Rat
    Sample Type: Conditioned Media

  15. Buijs M., Geschwing J., Syed L., et al. Spontaneous tumor regression in a syngeneic rat model of liver cancer: implications for survival studies. J Vasc Interv Radiol. 2012 Dec;23(12):1685-91. doi: 10.1016/j.jvir.2012.08.025.
    Species: Rat
    Sample Type: Serum

  16. Wirrig C, Hunter I, Mathieson FA, Nixon GF. Sphingosylphosphorylcholine is a proinflammatory mediator in cerebral arteries. Journal of Cerebral Blood Flow & Metabolism. 2011;31(1):212-221. doi:10.1038/jcbfm.2010.79.
    Species: Rat
    Sample Type: Conditioned Media

  17. Zhou J, Li J, Rosenbaum DM, Barone FC. Thrombopoietin protects the brain and improves sensorimotor functions: reduction of stroke-induced MMP-9 upregulation and blood-brain barrier injury. Journal of Cerebral Blood Flow & Metabolism. 2011;31(3):924-933. doi:10.1038/jcbfm.2010.171.
    Species: Rat
    Sample Type: Tissue lysate

  18. Zhang H, Yang R, Wang Z, Lin G, Lue TF, Lin C-S. Adipose Tissue-Derived Stem Cells Secrete CXCL5 Cytokine with Neurotrophic Effects on Cavernous Nerve Regeneration. The journal of sexual medicine. 2011;8(2):437-446. doi:10.1111/j.1743-6109.2010.02128.x.

  19. Jung, Eun-Jung, et al. "Bone marrow-derived mesenchymal stromal cells support rat pancreatic islet survival and insulin secretory function in vitro." Cytotherapy 13.1 (2011): 19-29.

  20. Cinova, Jana, et al. "Role of intestinal bacteria in gliadin-induced changes in intestinal mucosa: study in germ-free rats." PloS one 6.1 (2011): e16169.

  21. Nakano N., Nakai Y., Seo T., Yamada Y., Ohno T., Yamanaka A., et al. Characterization of conditioned medium of cultured bone marrow stromal cells. Neuroscience Letters 483 (2010) 57-61
    Species: Rat
    Sample Type: Conditioned Media

  22. Dagenais A, Frechette R, Clermont M, Masse C,Privé A,et al. Dexamethasone inhibits the action of TNF on ENaC expression and activity.Am J Physiol Lung Cell Mol Physiol. 2006;291:1220-1231,
    Species: Rat
    Sample Type: Conditioned Media

  23. Schmelzer K, Wheelock Å, Dettmer K , Morin D, Hammock B.The role of Inflammatory Mediators in the Synergistic Toxicity of Ozone and 1-Nitronaphthalene in Rat Airways.Environ Health Perspect. 2006 ; 114(9): 1354-1360.
    Species: Rat
    Sample Type: BAL Fluid

  24. Liao, H et al. Tenascin-R plays a role in neuroprotection via its distinct domains that coordinate to modulate the microglia function. Tenascin-R plays a role in neuroprotection via its distinct domains that coordinate to modulate the microglia function. Tenascin-R plays a role in neuroprotection via its distinct domains that coordinate to modulate the microglia function. J. Biol. Chem. Volume 280, Issue 9, Pages 8316-23, Mar 2005
    Species: Rat
    Sample Type: Conditioned Media

  25. Moreau, R et al. Reversal by aminoguanidine of the age-related increase in glycoxidation and lipoxidation in the cardiovascular system of Fischer 344 rats. Reversal by aminoguanidine of the age-related increase in glycoxidation and lipoxidation in the cardiovascular system of Fischer 344 rats. Reversal by aminoguanidine of the age-related increase in glycoxidation and lipoxidation in the cardiovascular system of Fischer 344 rats. Biochem. Pharmacol. Volume 69, Issue 1, Pages 29-40, Jan 2005
    Species: Rat
    Sample Type: Serum


Analysis Tool Bundle Discount

Specify options
1 x $283.00
+
1 x $115.00 $34.50

70% off the data analysis tool when you purchase the array and tool together!


Note: If you are not placing this order online, make sure to mention this bundle discount to our orders department (orders@raybiotech.com).

Total list price $398.00
Price for all $317.50

Note: If you are asking about pricing and you are outside of the US please contact your local RayBiotech distributor.

 

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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