Custom Antibody

 
 

 

Download Custom Antibody Order Form


Outline of Antibody Generation Service

Service Items

Time Description Price
Part 1. Polyclonal Antibody 5-10 Weeks Recognizing multiple antigenic sites of injected immunogen, immunization, high ELISA titer, affinity purity $600
Part2. Monoclonal Antibody 4-5 months recognizing one antigenic binding site of the antigen, Immunization, fusion, screening, subclone, characterization, affinity purity $3,900
Part 3. Antibody Purification and Conjugation 2-3 days conjugating polyclonal, monoclonal and recombinant antibodies, with Enzymes, fluorochromes, affinity ligand and solid surface conjugation Inquire

 

 
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Part 1. Polyclonal Antibody

Service items Time Criteria Price
Anti-regular peptide antibody 5 weeks 1. 4 mg of peptide from customer $600
2. Immunization of two rabbits
3. 100 ml of immune sera
4. Generated antibody recognizes immunogen in ELISA assay
5.  ELISA titer is up to 1:10000
6. Antibody purification by affinity column may be obtained upon request
Anti-regular peptide antibody 8 weeks 1. Design, synthesize and conjugate peptide(15-20mer,5-10mg) $1,200
2. Immunization of two rabbits
3. 100 ml of immune sera
4. Generated antibody recognizes immunogen in ELISA assay
5.  ELISA titer is up to 1:10000
6. Antibody purification by affinity column may be obtained upon request
Anti-phospho-peptide antibody 8-10 weeks 1. Design, synthesize, specific site phophorylation and conjugate  peptide(12-15mer,5-10mg) $2,300
2. Immunization of two rabbits
3. 100 ml of immune sera
4. Generated antibody recognizes immunogen in ELISA assay
5.  ELISA titer is  1:1000
6. Antibody purification by affinity column may be obtained upon request
Anti-protein antibody 5-7 weeks 1. 5 mg of purified Protein from customer $600
2.  Immunization of two rabbits
3. 100 ml of immune sera
4. Generated antibody recognizes immunogen in ELISA assay
5. ELISA titer is up to 1:8000
6. Antibody purification by affinity column may be obtained upon request
 
 

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Part 2. Monoclonal Antibody

 
Service Items Time

Criteria

Price
Anti-regular peptide antibody (customer provide antigen) 4-5 months 1.  Customer provide peptide(4mg) $3,900
2.  Immunization of two mice, fusion, screening, subclone and initial characterization
3.  With up to three monoclones and their supernatant for confirming detection
4.  Generated antibody recognizes immunogen in ELISA assay
5.  Purify Antibody from 100 ml of hybridoma culture supernatant or 1 ml of ascites fluid
 
Anti-regular peptide antibody (RayBiotech provide antigen) 4-5 months 1.  Design, synthesize and conjugate  peptide(15-20mer,5-10mg) $4,600
2.  Immunization of two mice, fusion, screening, subclone and initial characterization
3.  With up to three monoclones and their supernatant for confirming detection
4.  Generated antibody recognizes immunogen in ELISA assay
5.  Purify Antibody from 100 ml of hybridoma culture supernatant or 1 ml of ascites fluid
 
 
Anti-phospho-peptide antibody 5-6 months 1. Design, synthesize, specific site phophorylation and conjugate peptide(11-15mer, 5 mg) $5,600
2.  Immunization of two mice, fusion, screening, subclone and initial characterization
3.  With up to three monoclones and their supernatant for confirming detection
4.  Generated antibody recognizes immunogen in ELISA assay
5. Antibody purification by affinity column(1-5mg) may be obtained upon request
 
Anti-protein antibody 5 months 1.  4mg of purified Protein from customer $3,990
2.  Immunize two mice, fusion, screening, subclone and initial characterization
3.  with up to three monoclones and their supernatant for confirming detection
4. Generated antibody recognizes immunogen in ELISA assay
5. Antibody purification by affinity column(1-5mg) may be obtained upon request
 
 

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