RayBio® Human Protein Array Chip (2, 4, or 8 arrays) 1,000 X Biotin-conjugated anti-IgG: 1.5 ul / vial of Biotin-conjugated anti-mouse IgG, 1.5 ul / vial of Biotin-conjugated anti-rabbit IgG, 1.5 ul / vial of Biotin-conjugated anti-human IgG, 2 X Blocking Buffer (4 ml) |
20 X Wash Buffer I (15 ml) 20 X Wash Buffer II (15 ml) 2 X Binding Buffer (RIPA buffer, 20 mM Tris, pH 7.5, 0.3 M NaCl, 2% sodium deoxycholate, 2% TX-100) 10 ml 1,000 X Alexa Fluor 555-Conjugated Streptavidin (1.5 ul) |