Custom Protein

Our Custom Protein Service covers all aspects of recombinant protein development, including:

From Gene to Protein Expression

Cloning or Sub-cloning of Gene of Interest (GOI)

DNA Sequencing Verification of Recombinant Expression Construct

Gene Expression

Protein Affinity-Purification

Desalting, Aliquoting, Lyophilization, etc.

Ready for Customer

Phase Service	Timeline	Description	Deliverables
Phase 1: Gene cloning	1 week	Use customer’s expression vectors without any modification	N/A
		Gene synthesis and codon optimization	Recombinant expression vector DNA, 2 µg
		Subclone customer's gene into an expression vector	DNA sequencing chormatography data
Phase 2: Large-scale gene expression	1 week	Transform DNA sequence-confirmed recombinant plasmid into bacteria	Bacterial glycerol stocks transformed with recombinant expression vector, 2 vials, 0.5 mL/vial.
		Express protein in 2 liters of culture
		Collect, lyse and extract the induced cells
		Centrifuge to obtain the supernatant fractions for purification
Phase 3: Protein affinity purification	1 week	Protein affinity chromatography purification	Comprehensive report including induction conditions, method of extraction, SDS-PAGE data, protein concentration, etc.
		Determine the protein purity by SDS-PAGE with Coomassie blue staining	Affinity purified proteins (95% purity guaranteed)
		Protein desalting	Aliquoting, lyophilization and conjugation of purified proteins are available upon request
		Determine the protein concentration
		Concentrate the samples if protein concentration is low

Additional services
	1. Biotinylation of purified protein
	2. Proteolytic cleavage and fragment purification
	3. Site-directed mutagenesis in customer-provided plasmid
	4. Antibody purification
	5. Protein assay development and validation

We can tailor a work plan to suit your needs. To obtain a quotation, fill out a Service Request Form or contact us directly.