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Product: RayBio® Cytokine Antibody Arrays |
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Introduction
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As a protein array pioneer company, RayBiotech provides
the most comprehensive cytokine antibody array products in
today's market. Using our patent- pending technology, researchers
can rapidly and accurately measure the expression levels of up
to 200 cytokines, chemokines, growth factors, angiogenic
factors, proteases, soluble receptors and other proteins from
a small amount of samples in a single experiment.
The technology is designed based on the sandwich
immunoassay principle. A panel of antibodies (capture) is
immobilized in specific spot locations on the surface of
membrane. Incubation of array membranes with biological
samples results in the capture of cytokines by corresponding
antibodies. The bound cytokines are detected with a cocktail
of biotinylated antibodies. Signals are then visualized using
chemiluminescence or colorimetry or infrared fluorescence.
Many
biological processes such as apoptosis, inflammation,
angiogenesis, immunoresponse and migration often accompany changes
of cytokine expression levels. Cytokine profiles are also
altered in disease status such as cancer, arthritis,
atherosclerosis, obesity, diabetes and infection diseases.
Thus, RayBiotech’s cytokine antibody arrays have broad
applications in basic biomedical research, drug discovery and
biomarker discovery.
Our standard products apply
chemiluminescence detection. If you prefer colorimetric
detection or Infrared detection, please contact us at
1-888-494-8555. We will provide special kits or protocols for
you.
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Highlight
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Simultaneous
detection of multiple cytokines and other proteins in
a single experiment
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No
special equipment required
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High
sensitivity that detects most proteins at pg levels
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Requires
as little as 100 micro-liter
of sample to detect over 174 cytokines
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Affordable
and cost effective enough for routine use
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Easy
to use with minimal experience
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Diversity
of products: including human, mouse and rat
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Affordable, quick and
simple to use |
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High
specificity
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•Can
be used in diversity of biological samples
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Signal
can be detected in different ways: chemiluminescence,
colorimetry and infrared
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•Many
choices. Select your interesting protein for custom
arrays
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Contents
of kit |
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•RayBio® Cytokine Antibody Array membranes
(2/4/8 membranes)
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•Biotin-Conjugated
Anti-Cytokines
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•HRP-Conjugated
Streptavidin
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Blocking
Buffer
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Detection
Buffer C
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Detection
Buffer D
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•Eight-Well
Tray (1 each)
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Examples
of Potential Applications of Cytokine Antibody Arrays
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Profiling
cytokine expressions
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Monitoring
cytokine or chemokine levels in clinical trials
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Identifying
potential molecular targets for drug development
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Identifying
the molecular mechanisms of drug action
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Identifying
crucial factors involved in disease processes
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Discovering
biomarkers for disease management
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Discovering
expression patterns for molecular classification
of
diseases
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High Quality Control:
Specificity
and sensitivity are two major concerns for designing protein
arrays using antibodies as capture and detection reagents. At
RayBiotech, we only select antibodies with the highest
specificity and affinity in our array system. All antibodies
used in our system are rigidly tested and must meet our
superior standards. Examples below show the highest standard
of array systems.
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High Sensitivity:
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A recombinant protein IL-8 was incubated
with an array membrane spotted with 82 capture
antibodies. The signal was only detected in the IL-8
antibody spot. All antibodies in the system were tested
in this way. |
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High Reproducibility:
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Cytokines are detected with very high
sensitivity using the array format. Cytokine Antibody
Array membranes were incubated with different
concentrations of MCP-1. The intensities of the signals
were measured by densitometry and plotted against the
concentrations of MCP-1. |
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High Reproducibility:
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High reproducibility is important in
experimentation. The human Cytokine Antibody Array
system I was probed with a conditioned medium. Three
membranes showed similar signal intensities. |
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Comparable to ELISA Data:
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The relative expression levels in protein
arrays were determined by densitometry. The actual
amounts of protein levels were quantified by ELISA. Fold
change represents cytokines differentially expressed
between TNF? treated and untreated conditioned media
(U251 cells), and between patient sera number 4 and
number 11. |
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Proven Reliability:
Hundreds
of publications such as Nature, Lancet and PNAS by many
leading institutes around the world.
click for reference list...
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Papers
Citing Our Products (partial list)
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1.
Agrawal,N., Bettegowda,C., Cheong,I.,
Geschwind,J.F., Drake,C.G., Hipkiss,E.L.,
Tatsumi,M., Dang,L.H., Diaz,L.A., Jr., Pomper,M.,
Abusedera,M., Wahl,R.L., Kinzler,K.W., Zhou,S.,
Huso,D.L., and Vogelstein,B. (2004). Bacteriolytic
therapy can generate a potent immune response
against experimental tumors. Proc Natl Acad Sci U
S A. 101, 15172-15177.
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| 2. Carroll,T.P., Greene,C.M.,
Taggart,C.C., Bowie,A.G., O'Neill,S.J., and
McElvaney,N.G. (2005). Viral inhibition of IL-1- and
neutrophil elastase-induced
inflammatory responses in bronchial epithelial cells.
J Immunol. 175, 7594-7601. |
| 3. De,S., Razorenova,O., McCabe,N.P.,
O'Toole,T., Qin,J., and Byzova,T.V. (2005).
VEGF-integrin interplay controls tumor growth and vascularization.
Proc Natl Acad Sci U S A. 102, 7589-7594. |
| 4. Kim,B.G., Li,C., Qiao,W., Mamura,M.,
Kasperczak,B., Anver,M., Wolfraim,L., Hong,S.,
Mushinski,E., Potter,M., Kim,S.J., Fu,X.Y., Deng,C.,
and Letterio,J.J.
(2006). Smad4 signalling in T cells is required for
suppression of gastrointestinal cancer. Nature. 441,
1015-1019. |
| 5. Marks,D.J., Harbord,M.W.,
MacAllister,R., Rahman,F.Z., Young,J., Al-Lazikani,B.,
Lees,W., Novelli,M., Bloom,S., and Segal,A.W. (2006). Defective
acute inflammation in Crohn's disease: a clinical
investigation. Lancet. 367, 668-678. |
| 6. Tang,X., Marciano,D.L., Leeman,S.E.,
and Amar,S. (2005). LPS induces the interaction of a
transcription factor, LPS-induced TNF-alpha factor, and
STAT6(B) with effects on multiple cytokines. - Proc
Natl Acad Sci U S A. 102, 5132-5137.
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| 7. Wang,F.X., Xu,Y., Sullivan,J.,
Souder,E., Argyris,E.G., Acheampong,E.A., Fisher,J.,
Sierra,M., Thomson,M.M., Najera,R., Frank,I.,
Kulkosky,J., Pomerantz,R.J.,
and Nunnari,G. (2005). IL-7 is a potent and proviral
strain-specific inducer of latent HIV-1 cellular
reservoirs of infected individuals on virally
suppressive HAART. J Clin Invest. 115, 128-137. |
| 8. Xu,Y., Kulkosky,J., Acheampong,E.,
Nunnari,G., Sullivan,J., and Pomerantz,R.J. (2004).
HIV-1-mediated apoptosis of neuronal cells: Proximal molecular
mechanisms of HIV-1-induced encephalopathy. Proc Natl
Acad Sci U S A 101, 7070-7075. |
| 9. Yang,F.C., Ingram,D.A., Chen,S.,
Hingtgen,C.M., Ratner,N., Monk,K.R., Clegg,T., White,H.,
Mead,L., Wenning,M.J., Williams,D.A., Kapur,R., Atkinson,S.J.,
and Clapp,D.W. (2003). Neurofibromin-deficient Schwann
cells secrete a potent migratory stimulus for Nf1+/-
mast cells. J Clin Invest. 112, 1851-1861.
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| 10. Yen,Y.T., Liao,F., Hsiao,C.H.,
Kao,C.L., Chen,Y.C., and Wu-Hsieh,B.A. (2006).
Modeling the early events of severe acute respiratory
syndrome coronavirus
infection in vitro. J Virol. -93. |
11. Huang RP. (2007). An array of
possibilities in cancer research using cytokine
antibody arrays.
Expert
Rev Proteomics. 4(2):299-308. |
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