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Human Phospho-DDR1 (Y792) ELISA

CODE: PEL-DDR1-Y792-1

$491.00

RayBio® Human Phospho-DDR1 (Tyr792) ELISA Kit. This assay semi-quantitatively measures DDR1 phosphorylated at Tyrosine-792 in lysate samples.


Product Features

  • Rapidly measure phosphorylated protein in lysates
  • Screen numerous different cell lysates without performing a Western Blot analysis
  • Minimal hands-on time, convenient, and non-radioactive material

Specificity

The antibody pair provided in this kit recognizes Human DDR1 phosphorylated at site Tyrosine-792.

Target Protein Information

Synonyms
Epithelial discoidin domain-containing receptor 1 (Epithelial discoidin domain receptor 1) (EC 2.7.10.1) (CD167 antigen-like family member A) (Cell adhesion kinase) (Discoidin receptor tyrosine kinase) (HGK2) (Mammary carcinoma kinase 10) (MCK-10) (Protein-tyrosine kinase 3A) (Protein-tyrosine kinase RTK-6) (TRK E) (Tyrosine kinase DDR) (Tyrosine-protein kinase CAK) (CD antigen CD167a)
Accession Number
Gene Names
DDR1 CAK EDDR1 NEP NTRK4 PTK3A RTK6 TRKE
Gene ID
780

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Anti-Phospho Antibody
  • HRP-Conjugated Secondary Antibody
  • Assay Diluent
  • TMB One-Step Substrate
  • Stop Solution
  • Lysis Buffer
  • Positive Control Sample
Other Materials Required
  • Distilled or deionized water
  • 100 ml and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 µl to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm
Protocol Outline
  1. Prepare all reagents and samples as instructed in the manual.
  2. Add 100 µl of sample or positive control to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 µl of prepared primary antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared 1X HRP-Streptavidin to each well.
  7. Incubate 1 h at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Typical Data

Positive Control
Jurkat cells were treated with Pervanadate. Solubilize cells at 4 x 10^7 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA. Please see step 3 of Part VI Reagent Preparation for detail.
Calyculin A and Pervanadate (PV) Stimulation of Jurkat Cell Line
Jurkat cells were treated with Calyculin A and Pervanadate. Cell lysates were analyzed using this phosphoELISA and Western Blot.


Storage/Stability

Upon receipt, the kit should be stored at –20°C. Please use within 6 months from the date of shipment.

Product Specifications

  • 1, 2, or 5 x 96-Well Strip Microplate Kit

Array/ELISA Format

  • p53 Signaling Pathway
  • Tyrosine Kinase Family
  • Human
  • 96-well Microplate
  • Sandwich-based
  • Semi-Quantitative
  • Colorimetric
  • Cell Lysates
  • Tissue Lysates

Note: If you are asking about pricing and you are outside of the US please contact your local RayBiotech distributor.

 

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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