G-Series: Glass Slide-Based Arrays

Semi-Quantitative, Sandwich-based, Glass Slide Antibody Arrays

Antibody arrays allow researchers to conduct rapid, accurate expression profiling of hundreds of cytokines, chemokines, growth factors, proteases, soluble receptors and other proteins from any biological fluid. 

RayBio® Glass Slide-based Antibody Arrays (G-Series) were introduced as a “second generation” array platform after the chemiluminescent membrane arrays (C-Series). The G-Series uses a 75mm x 25mm glass chip platform which delivers easier handling along with the use of much lower sample volumes. The glass slides also utilize a fluorescent signal readout, allowing a wider dynamic detection range than can be achieved by chemiluminescence.

G-Series Antibody Arrays are particularly well-suited for projects with very limited sample volumes (as little as 10 μl per array) and large numbers of samples. Dozens of arrays can be processed per day by a single technician. Furthermore, the slide chamber assembly is compatible with many automated array processing systems, allowing high-throughput studies to be performed with very little hands-on time.

Features

Research Applications

• High sensitivity (pg/mL to ng/mL levels)
• Large dynamic range of detection (4 orders of magnitude)
• Compatible with many sample types
• High throughput
• Low sample consumption: as little as 10 µl of original sample required per array
• Customizable – create a custom array from our list of targets

• High-throughput profiling of cytokine expression
• Identifying potential molecular targets for drug development
• Identifying the molecular mechanisms of drug action<
• Identifying crucial factors involved in disease processes
• Discovering biomarkers for disease management
• Discovering expression patterns for molecular classification of diseases

Similar to the C-Series (membrane-based) arrays, G-Series Antibody Arrays utilize the sandwich-ELISA design principle. In this assay, capture antibodies are printed in multiple identical arrays on a standard-sized histology slide. After a blocking step, samples are incubated with the arrays. Nonspecific proteins are then washed off, and the arrays are incubated with a cocktail of biotinylated detection antibodies, followed by a streptavidin-conjugated fluor. Signals are visualized using a fluorescence laser scanner.

For a list of laser scanner specifications, click here.

If you do not have access to a scanner, RayBiotech provides slide scanning and data analysis services