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High Throughput Plate Reader Service

Synergy H4 Hybrid Multi-Mode Microplate Reader

Read Method
End point, kinetic, spectral scanning, well-area scanning
Microplate Types
Monochromator system 1- to 384-well plates, Filter system 1- to 1536-well plates, PCR plates, Compatible with Take3™ Multi-Volume Plate with 2 µL microspots
Temperature Control
4°C above ambient to 65°, C + 0.5°C at 37°C
Shaking
Yes

 

Fluorescence intensity (FI)

As a result of the illumination, the sample emits light (it fluoresces) and a second optical system collects the emitted light, separates it from the excitation light (using a filter or monochromator system), and measures the signal using a light detector.

Sensitivity
Monochromators: Fluorescein 2 pM typical (0.2 fmol/well; 384-well plate) (Top); Fluorescein 2.5 pM typical (0.25 fmol/well; 384-well plate) (Bottom)
Filters/mirrors: Fluorescein 1 pM typical (0.1 fmol/well; 384-well plate)
Wavelength Range
Monochromators: 250 - 850 nm Filters: 200 - 700 nm (850 nm option)
Dynamic Range
Monochromators: 5 decades; Filters/mirrors: >6 decades
Reading Speed
96: 11 seconds; 384: 22 seconds; 1536: 42 seconds

 

Fluorescence Polarization (FP)

Fluorescence Polarization can be used to measure the binding constants and kinetics of reactions that cause a change in the rotational time of the molecules. If the fluorophore is bound to a small molecule, the rate at which it tumbles can decrease significantly when it is bound tightly to a large protein. If the fluorophore is attached to the larger protein in a binding pair, the difference in polarization between bound and unbound states will be smaller

Sensitivity
3 mP at 1 nM fluorescein typical
Wavelength Range
400 - 700 nm (320 - 850 nm option)

 

Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET)

HTRF (TR-FRET) combines two techniques FRET (Fluorescence Resonance Energy Transfer) which is homogeneous and TRF (Time Resolved Fluorescence) which is low background. Advantages of TR-FRET: high sensitivity, high throughput, low false-positive, reliable results and easy protocol.

Sensitivity
Europium 60 fM typical with filters (6 amol/well in 384-well plate)
Wavelength Range
Filters: 200 - 700 nm (850 nm option); Monochromators: 250 - 850 nm

 

High-Performance Luminescence

Detect the emission of cold body strong light signal by a substance not resulting from heat.

Sensitivity
10 amol ATP typical (flash)
Wavelength Range
300 - 700 nm
Dynamic Range
>6 decades

 

UV-Visible Absorbance

Molecules containing π-electrons or non-bonding electrons (n-electrons) can absorb the energy in the form of ultraviolet or visible light to excite these electrons to higher anti-bonding molecular orbitals. The more easily excited the electrons (i.e. lower energy gap between the HOMO and the LUMO), the longer the wavelength of light it can absorb.

Wavelength Range
230 - 999 nm, 1 nm increment
Bandpass
2 nm (230-285 nm), 4 nm (>285 nm)
Dynamic Range
0 - 4.0 OD
Resolution
0.0001 OD; Monochromator wavelength accuracy: +2 nm; Monochromator wavelength repeatability: +0.2 nm; Reading speed: 96: 11 seconds; 384: 22 seconds
Minimum Sample Amount
2 μL microspots for low volume assays
 

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