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Human/Mouse/Rat Apelin EIA

CODE: EIA-APC-1

$358.00

RayBio® Human/Mouse/Rat Apelin EIA Kit for serum, plasma, culture supernatants and cell lysates.

Manual                EIA Overview


Product Feature

  • High specificity and reproducibility
  • Adipokines, hormones and more detected at pg/ml and pmol/L levels
  • Easy to perform and rapid results
  • Diverse biological samples
  • Low sample volume required
  • Unique targets; no other assays available

Target Name

APC

Target Species

Human

Species Detected

Human, Mouse, Rat

Application Notes

Suggested Application
Quantitative Protein Detection; Establishing Normal Range; Validation of Antibody Array Results
Kit Components
  • Pre-Coated 96-Well Strip Microplate
  • Wash Buffer
  • Standard Peptide
  • Assay Diluent(s)
  • Biotinylated Peptide
  • HRP-Streptavidin
  • TMB One-Step Substrate
  • Stop Solution
  • Assay Diagram
  • Positive Control Sample
  • Capture Antibody
  • User Manual
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μl to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • 100 ml and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Orbital shaker
  • Aluminum foil
  • Saran Wrap
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • SigmaPlot software (or other software that can perform four-parameter logistic regression models)
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed.
  2. Add 100 μl detection antibody to each well. Incubate 1.5 h at RT or O/N at 4°C.
  3. Add 100 μl standard or sample to each well. Incubate 2.5 h at RT.
  4. Add 100 μl prepared streptavidin solution. Incubate 45 min at RT.
  5. Add 100 μl TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT.
  6. Add 50 μl Stop Solution to each well. Read plate at 450 nm immediately.

Specificity

The capture antibody provided in this kit recognizes human, mouse and rat Apelin.

Product Specifications

One 96-Well Strip Microplate Kit

Target Information

  • Human Human

Array/ELISA Format

  • 96-well Microplate 96-well Microplate
Competition-based
Quantitative
  • Colorimetric Colorimetric
  • Cell Culture Supernatants Cell Culture Supernatants
  • Cell Lysates Cell Lysates
  • Plasma Plasma
  • Serum Serum

Manual [Download]

MSDS [Download]

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.
 
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