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Human Apoptosis Signaling Array C1

CODE: AAH-APOSIG-1-2

$386.00

RayBio® C-Series Human Apoptosis Antibody Array 1 Kit. Detects 19 phosphorylated or cleaved human Apoptotic Factors. Suitable for all liquid sample types but intended for use with cell and tissue lysates.


Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information

Akt (P-Ser473)
(PKB (P-Ser473))
ATM (P-Ser1981)
BAD (P-Ser112)
Caspase-3 (Cleaved Asp175)
Caspase-7 (Cleaved Asp198)
  • Gene Symbol: CASP7 MCH3
  • Gene ID: 840
  • Accession #: P55210
CHK1 (P-Ser296)
  • Gene Symbol: CHEK1 CHK1
  • Gene ID: 1111
  • Accession #: O14757
CHK2 (P-Thr68)
eIF-2a (P-Ser52)
ERK1 (P-T202/Y204)/ERK2 (P-Y185/Y187)
HSP27 (P-Ser82)
IKBa (P-Ser32)
JNK (P-Thr183/Tyr185)
NFKBP65 (P-Ser536)
PARP1 (Cleaved Asp214/Gly215)
p27 (P-Thr198)
P53 (P-Ser15)
SMAD2 (Ser245/250/255)
TAK1 (P-Ser412)

Species Detected

Human

Typical Results

Figure 1: HeLa cells were grown to 80% confluency and then serum starved overnight. Cells were either untreated (bottom panel) or treated (top panel) with Staurosporine (STS) for 3 hours. Data shown are from a 20 second exposure using a chemiluminescence imaging system.

Figure 2: Jurkat cells were either untreated (bottom panel) or treated (top panel) with Camptothecin(CPT) for 16 hours. Data shown are from a 20 second exposure using a chemiluminescence imaging system.

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Apoptosis Signaling Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Detection Antibody Cocktail
  • HRP-Conjugated anti-IgG
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • Protease Inhibitor Cocktail
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated anti-IgG
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Number of Targets Detected

19

Product Specifications

  • 2, 4, or 8 Sample Kit

Array/ELISA Format

  • Akt Signaling Pathway
  • HIF-1 alpha Signaling Pathway
  • IGF Signaling Pathway
  • NFkB Signaling Pathway
  • p53 Signaling Pathway
  • PI3K Signaling Pathway
  • TGF-beta Signaling Pathway
  • Wnt/beta-Catenin Signaling Pathway
  • JAK/STAT Signaling Pathway
  • MAPK Signaling Pathway
  • mTOR Signaling Pathway
  • Human
  • Membrane
  • Sandwich-based
  • Semi-Quantitative
  • Chemiluminescence
  • Serum
  • Plasma
  • Cell Culture Supernatants
  • Cell Lysates
  • Tissue Lysates

Analysis Tool Bundle Discount

Specify options
1 x $386.00
+
1 x $115.00 $34.50

70% off the data analysis tool when you purchase the array and tool together!


Note: If you are not placing this order online, make sure to mention this bundle discount to our orders department (orders@raybiotech.com).

Total list price $501.00
Price for all $420.50

Note: If you are asking about pricing and you are outside of the US please contact your local RayBiotech distributor.

 

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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