1-888-494-8555

Human BMP Related Array 1

CODE: AAH-BMP-1-2

$320.00

RayBio® C-Series Human BMP Related Array 1 Kit. Detects 16 Human BMPs. Suitable for all liquid sample types.


Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information

BAMBI
(NMA)
BMP-2
BMP-4
BMP-5
BMP-6
BMP-7
BMP-8
BMP-9
BMPR-IA
(ALK-3)
ALK-6
(BMPR-IB)
BMPR-II
COCO
Crossveinless-2
(CV-2/BMPER)
Follistatin
GDF-11
(BMP-11)
GDF-8
(Myostatin)

Species Detected

Human

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human BMP Related Array 1
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Number of Targets Detected

16

Product Specifications

  • 2, 4 or 8 Sample Kit

Array/ELISA Format

  • Human
  • Membrane
  • Sandwich-based
  • Semi-Quantitative
  • Chemiluminescence
  • Serum
  • Plasma
  • Cell Culture Supernatants
  • Cell Lysates
  • Tissue Lysates

  1. Perrigue P., Silva M., Warden C., et al. The histone demethylase jumonji coordinates cellular senescence including secretion of neural stem cell-attracting cytokines. Mol Cancer Res. 2015 Apr;13(4):636-50. doi: 10.1158/1541-7786.MCR-13-0268
    Species: Human
    Sample Type: Conditioned Media

  2. Salhi A., Farhadian J., Giles K., et al. RSK1 Activation Promotes Invasion in Nodular Melanoma. Am J Pathol. 2015 Mar;185(3):704-16. doi: 10.1016/j.ajpath.2014.11.021.
    Species: Human
    Sample Type: Tissue Lysate

  3. Huang J., Yang B., Xiang T., et al. Diallyl disulfide inhibits growth and metastatic potential of human triple-negative breast cancer cells through inactivation of the β-catenin signaling pathway. Mol Nutr Food Res. 2015 Jun;59(6):1063-75. doi: 10.1002/mnfr.201400668.
    Species: Human
    Sample Type: Conditioned Media

  4. Carduner L., Leroy-Dudal J., Picot CR., Gallet O., Carreiras F., et al. Ascites-induced shift along epithelial-mesenchymal spectrum in ovarian cancer cells: enhancement of their invasive behavior partly dependant on αv integrins. Clinical and Experimental Metastasis. Jun 2014 Epub Ahead of Print.
    Species: Human
    Sample Type: Peritoneal Fluid

  5. Ichikawa T., Sugiura H., Koarai A., Minakata Y., Kikuchi T., Morishita Y., et al. TLR3 Activation Augments Matrix Metalloproteinase Production through Reactive Nitrogen Species Generation in Human Lung Fibroblasts. The Journal of Immunology April 23, 2014 1302919 [Epub Ahead of Print]
    Species: Human
    Sample Type: Conditioned Media

  6. Krohn A., et al. Tumor Cell Heterogeneity in Small Cell Lung Cancer (SCLC): Phenotypical and Functional Differences Associated with Epithelial-Mesenchymal Transition (EMT) and DNA Methylation Changes. PLOS One Published: June 24, 2014 DOI: 10.1371/journal.pone.0100249
    Species: Human
    Sample Type: Conditioned Media

  7. Orgaz J., et al. Diverse matrix metalloproteinase functionsregulate cancer amoeboid migration. Nature Communications 5, Article number:4255. doi:10.1038/ncomms5255
    Species: Human
    Sample Type: Conditioned Media

  8. Patil P., Joshi M., Kuna Vijay., Xu B., Johannesson L., Olausson M., Sumitran-Holgersson S. CD271 identifies functional human hepatic stellate cells, which localize in peri-sinusoidal and portal areas in liver after partial hepatectomy. [Published same as previous]
    Species: Human
    Sample Type: Conditioned Media

  9. Sakimoto T., et al. Simultaneous study of matrix metalloproteinases, proinflammatory cytokines, and soluble cytokine receptors in the tears of noninfectious corneal ulcer patients. Graefes Arch Clin Exp Ophthalmol Pubslished online July 2014. DOI 10.1007/s00417-014-2708-1
    Species: Human
    Sample Type: Tears

  10. Differential secretome analysis of cancer-associated fibroblasts and bone marrow-derived precursors to identify microenvironmental regulators of colon cancer progression
    Species: Human
    Sample Type: Conditioned Media

  11. Differential secretome analysis of cancer-associated fibroblasts and bone marrow-derived precursors to identify microenvironmental regulators of colon cancer progression
    Species: Human
    Sample Type: Conditioned Media

  12. Lisboa RA, Andrade MV, Cunha-Melo. Toll-like receptor activation and mechanical force stimulation promote the secretion of matrix metalloproteinases 1, 3 and 10 of human periodontal fibroblasts via p38, JNK and NF-κ. Arch Oral Biol. 2013;58(6):731-739.
    Species: Human
    Sample Type: Conditioned Media

  13. PAR2-induced inflammatory responses in human kidney tubular epithelial cells
    Species: Human
    Sample Type: Conditioned Media

  14. Regenerative Potential of Decellularized Porcine Nucleus Pulposus Hydrogel Scaffolds: Stem Cell Differentiation, Matrix Remodeling, and Biocompatibility Studies
    Species: Human
    Sample Type: Conditioned Media

  15. Vesey DA, Suen JY, Seow V, et al. PAR2-induced inflammatory responses in human kidney tubular epithelial cells. Am J Physiol Renal Physiol. 2013 Mar 15;304(6):F737-F750.
    Species: Human
    Sample Type: Conditioned Media

  16. Yang B., et al. Chrysin inhibits metastatic potential of human triple-negative breast cancer cells by modulating matrix metalloproteinase-10, epithelial to mesenchymal transition, and PI3K/Akt signaling pathway. J Appl Toxicol. 2014 Jan;34(1):105-12. doi: 10.1002/jat.2941. Epub 2013 Oct 10
    Species: Human
    Sample Type: Cell Lysate

  17. Zhu S., Hong J., Tripathi M., Sehdev V., et al. Regulation of CXCR4-Mediated Invasion by DARPP-32 in Gastric Cancer Cells. Mol Cancer Res January 2013 11; 86
    Species: Human
    Sample Type: Conditioned Media

  18. Caino MC., Lopez-Haber C., Kissil JL., Kazanietz MG. Non-small cell lung carcinoma cell motility, rac activation and metastatic dissemination are mediated by protein kinase C epsilon. PLoS One. 2012;7(2):e31714. doi: 10.1371/journal.pone.0031714
    Species: Human
    Sample Type: Conditioned Media

  19. Connolly M., Mullan RH., McCormick J., et al. Acute-phase serum amyloid A regulates tumor necrosis factor α and matrix turnover and predicts disease progression in patients with inflammatory arthritis before and after biologic therapy. Arthritis Rheum. 2012 Apr;64(4):1035-45. doi: 10.1002/art.33455. Epub 2011 Nov 10.
    Species: Human
    Sample Type: Conditioned Media

  20. Li R, Ackerman WE, Mihai C, Volakis LI, Ghadiali S, Kniss DA. Myoferlin Depletion in Breast Cancer Cells Promotes Mesenchymal to Epithelial Shape Change and Stalls Invasion. Hong W, ed. PLoS ONE 2012;7(6):e39766. doi:10.1371/journal.pone.0039766.
    Species: Human
    Sample Type: Conditioned Media

  21. Abdul Muneer P., Alikunju S., Szlachetka A., Haorah J. The mechanisms of cerebral vascular dysfunction and neuroinflammation by MMP-mediated degradation of VEGFR-2 in alcohol ingestion. Arterioscler Thromb Vasc Biol. 2012 May;32(5):1167-77. doi: 10.1161/ATVBAHA.112.247668
    Species: Human
    Sample Type: Conditioned Media

  22. Ranzato E., Martinotti S., Burlando B., et al.
    Species: Human
    Sample Type: Conditioned Media

  23. Kummer N., Nowicki T., Azzi J., et al. Arachidonate 5 lipoxygenase expression in papillary thyroid carcinoma promotes invasion via MMP-9 induction. J Cell Biochem. 2012 Jun;113(6):1998-2008. doi: 10.1002/jcb.24069.
    Species: Human
    Sample Type: Conditioned Media

  24. Santosh N., et al. Matrix Metalloproteinase Expression During Blastema Formation in Regeneration-Competent Versus Regeneration-Deficient Amphibian Limbs. DEVELOPMENTAL DYNAMICS 240:1127-1141, 2011. DOI 10.1002/dvdy.22503
    Species: Xenopus
    Sample Type: Tissue Lysate

  25. Harper JI, Godwin H, Green A, Wilkes LE, Holden NJ, et al. A study of matrix metalloproteinase expression and activity in atopic dermatitis using a novel skin wash sampling assay for functional biomarker analysis. Br J Dermatol. 2010 Feb 1;162(2):397-403.
    Species: Human
    Sample Type: Skin Wash

  26. Raghu H., Lakka S., Gondi C., Mohanam S., et al. Suppression of uPA and uPAR Attenuates Angiogenin Mediated Angiogenesis in Endothelial and Glioblastoma Cell Lines. PLOS One, Published: August 27, 2010. DOI: 10.1371/journal.pone.0012458
    Species: Human
    Sample Type: Conditioned Media

  27. Steed D, Trumpower C, Duffy D, Smith C, Marshall V, et al. Amnion-derived Cellular Cytokine Solution A Physiological Combination of Cytokines for Wound Healing. Eplasty. 2008;8:e18.
    Species: Human
    Sample Type: Conditioned Media

  28. Thielitz A, Vetter R, Schultze B, Wrenger S, Simeoni L,et al.Inhibitors of Dipeptidyl Peptidase IV-Like Activity Mediate Antifibrotic Effects in Normal and Keloid-Derived Skin Fibroblasts. J Investig Dermatol. 2008;128:855-866
    Species: Human
    Sample Type: Conditioned Media

  29. Cortez DM, Feldman MD, Mummidi S, Valente AJ, Steffensen B, et al. IL-17 stimulates MMP-1 expression in primary human cardiac fibroblasts via p38 MAPK- and ERK1/2-dependent C/EBP-beta, NF-κ, and AP-1 activation. Am J Physiol Heart Circ Physiol. 2007;293:H3356-H3365.
    Species: Human
    Sample Type: Conditioned Media

  30. Del Carlo M, Schwartz D, Erickson EA, Loeser RF. Endogenous production of reactive oxygen species is required for stimulstion of human articular chondrocyte matrix metalloproteinase production by fibronectin fragments. Free Radical Biol Med. 2007;42:1350-1358.
    Species: Human
    Sample Type: Conditioned Media

  31. Smith GN Jr, Mickler EA, Payne KK, Lee J, et al. Lung Transplant Metalloproteinase Levels are Elevated Prior to Bronciolitis Obliterans Syndrome. Am J Transplant. 2007;7:1656-1661.
    Species: Human
    Sample Type: BAL Fluid

  32. Sze S, Kleijn D, Lai R, Tan E, Zhao H,et al.Elucidating the Secretion Proteome of Human Embryonic Stem Cell-derived Mesenchymal Stem Cells.Mol Cell Proteomics. 2007; 6: 1680-1693
    Species: Human
    Sample Type: Conditioned Media

  33. Chandrasekar B, Mummidi S, Mahimainathan L, Patel DN, Baile SR, et al. Interleukin-18-induced Human Coronary Artery Smooth Muscle Cell Migration is Dependent on NF-κ- and AP-1-mediated Matrix Metalloproteinase-9 Expression and is inhibited by Atorvastatin. J Biol Chem. 2006:281(22):15099-15109.
    Species: Human
    Sample Type: Conditioned Media

  34. Kanangat S, Postlethwaite A, Hasty K, Kang A, et al. Induction of multiple matrix metalloproteinases in human dermal and synovial fibroblasts by Staphylococcus aureau: Implications in the pathogenesis of septic arthritis and other soft tissue infections. Arthritis Res Ther. 2006;8:R176. Available at http://arthritis-research.com/content/8/6/R176. Last accessed January 17, 2008.
    Species: Human
    Sample Type: Conditioned Media


Analysis Tool Bundle Discount

Specify options
1 x $320.00
+
1 x $115.00 $34.50

70% off the data analysis tool when you purchase the array and tool together!


Note: If you are not placing this order online, make sure to mention this bundle discount to our orders department (orders@raybiotech.com).

Total list price $435.00
Price for all $354.50

Note: If you are asking about pricing and you are outside of the US please contact your local RayBiotech distributor.

 

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

Loading...