Protein phosphorylation plays an unusually prominent role in cell
signaling, development and growth. The RayBio® Human RTK Phosphorylation
antibody array kits are a very rapid, convenient and sensitive assay
to simultaneously detect multiple protein phosphorylation and can
be used to monitor activation or function of important biological
pathways. RayBiotech is committed to develop a series of phosphorylation
antibody arrays. Our first product in this series is RayBio® Human
RTK Phosphorylation antibody array I, which is specifically designed for
simultaneously identifying the relative levels of phosphorylation
of seventy-one different Human Receptor Tyrosine Kinases (RTKs)
in cell lysate. By monitor changes in protein tyrosine phosphorylation
in your experimental model system, you can verify pathway activation
in your cell lines without spending excess time and effort in performing
an analysis of immunoprecipitation and/or Western Blot.
By using
RayBio® Human
RTK Phosphorylation
antibody array I, treated or untreated cell lysate is added into antibody
array chip (either membranes or glass slides). The antibody array
chips are washed and biotinylated anti-phosphotyrosine antibody is
used to detect phosphorylated tyrosines on activated receptors. After
incubation with HRP-streptavidin or Alexa Fluor 555-streptavidin,
the signals are visualized by chemiluminescence or fluorescence imaging
system. |
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Receptor Tyrosine Kinases List: |
| ABL1 |
ACK1 |
ALK |
Axl |
Blk |
BMX |
| Btk |
Csk |
Dtk |
EGFR |
EphA1 |
EphA2 |
| EphA3 |
EphA5 |
EphA6 |
EphA7 |
EphA8 |
EphB1 |
| EphB2 |
EphB3 |
EphB4 |
EphB5 |
EphB6 |
ErbB2 |
| ErbB3 |
ErbB4 |
FAK |
FER |
FGFR1 |
FGFR2 |
| FGFR2
(α isoform) |
Fgr |
FRK |
Fyn |
Hck |
HGFR |
| IGF-I
R |
Insulin
R |
Itk |
JAK1 |
JAK2 |
JAK3 |
| LCK |
LTK |
Lyn |
MATK |
M-CSFR |
MUSK |
| NGFR |
PDGFR-α |
PDGFR-β |
PYK2 |
RET |
ROR1 |
| ROR2 |
ROS |
RYK |
SCFR |
SRMS |
SYK |
| Tec |
Tie-1 |
Tie-2 |
TNK1 |
TRKB |
TXK |
| Tyk2 |
TYRO10 |
VEGFR2 |
VEGFR3 |
ZAP70 |
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| RayBio®
Human RTK Phosphorylation Antibody Array (membrane-based): |
| |
| RayBio®
Phosphorylation Antibody Array (glass-chip based): |
| |
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| Example
of Potential Applications: |
|
|
Monitoring
cell signal transduction pathway, relative level of tyrosine phosphorylation
of RTKs and protein kinase activity |
|
|
Identifying
crucial factors involved in disease process |
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|
Discover
biomarkers for diagnosis and prognosis, involved in drug development |
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|
Screening
the effects of various treatments, activators or inhibitors in cell
signaling, development and growth |
