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Transcription Factor ELISA

The RayBio® Transcription factor-Enzyme Linked Immunosorbent Assays are non-radioactive transcription factor assays with an ELISA format. They offer an easy, speedy, sensitive and high-throughput method to detect the activation of transcription factors. Double stranded oligonucleotides are coated in a 96 well plate. These oligonucleotides specifically capture the target transcription factor contained in whole cell lysate or nuclear extracts after a short incubation. Subsequently, the primary antibody recognizes the target transcription factor-DNA complex in each well, and a HRP-conjugated secondary antibody is then used for detection. After washing away any unbound antibody, signal can be obtained easily through a colorimetric assay with a spectrophotometric plate reader at 450 nm. The specificity of the reaction between the target transcription factor and the DNA probe is additionally stringent because of the establishment of specific competitive DNA and non-specific competitive DNA probes in this reaction system.

  Product Product code Price  
Human NF-KappaB p65 Transcription Factor ELISA

CODE: TFEH-p65-1

$398.00
Human NF-KappaB p50 Transcription Factor ELISA

CODE: TFEH-p50-1

$398.00
Human HIF-1alpha Transcription Factor ELISA

CODE: TFEH-HIF1a-1

$398.00
Human NRF2 Transcription Factor ELISA

CODE: TFEH-NRF2-1

$398.00
Mouse NRF2 Transcription Factor ELISA

CODE: TFEM-NRF2-1

$398.00
Human pCREB Transcription Factor ELISA

CODE: TFEH-pCREB-1

$398.00
Human OCT4 Transcription Factor ELISA

CODE: TFEH-OCT4-1

$398.00
Human SOX2 Transcription Factor ELISA

CODE: TFEH-SOX2-1

$398.00
Human NANOG Transcription Factor ELISA

CODE: TFEH-NANOG-1

$398.00
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