Introduction Features       Product Custom Array Testing Service FAQ

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Less sample, more data:

Only 50 µl sample is needed for quantification of up to 20 cytokines in quadruplicate. This is 80 times more efficient than traditional ELISA. The advantage of Quantibody array is even precious when sample supply is limited.

Less time

The processing time for one slide is around 4 hours. Because of the high-throughput capability of the array, hundreds of samples can be processed by one technician in a single day.

Much lower cost

In order to get triplicate data results, the average cost per cytokine per sample for traditional ELISA is normally over $13 in the 96-well plate format. However, in Quantibody Arrays, even the quadruplicate data results will only cost less than $3 per cytokine per sample for a 10-cytokine array. This is much affordable and cost effective.

ELISA detection sensitivity

ELISA is well-known for its sensitivity and specificity in cytokine quantification. Our Quantibody products use sandwich-ELISA based methology and have been tested to have similar detection sensitivity as traditional ELISA.

Reproducibility

Quantibody products use photobleaching resistant Alexa dyes for detection, which greatly increases the system reproducibility and dynamic detection sensitivity. The slides can be rescanned for multiple purposes and stored in dark for months without loss of sensitivity.

Flexibility

Our available kits include either 10 or 20 defined cytokines in the detection panel. However, our custom arrays enable our users to choose not only the cytokine kinds but also the number of cytokines to be included in the array from our several hundred of cytokine pools. In addition, users can choose Alexa 647 dye (red, cy5 channel) for detection instead of Fluorescent dye (green, cy3 channel).

Quantitative analysis software available

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1.      What is included in the product?

Our Quantibody product is provided as a kit, which includes all the necessary reagents for performing the experiments. The materials include:

·        Glass Chip with antibody arrays

·        Sample Diluent

·        Lyophilized cytokine standard mix

·        Detection antibody cocktail

·        Streptavidin-Fluorescent dye

·        Wash buffer

·        Manual

2.       What equipment do I need for imaging?

A standard glass slide laser scanner with cy3- or cy5-compatible wavelength is needed for the signal detection. User can choose Fluorescent dye (green, cy3 channel, 555nm excitation, 565nm emission)  signal detection.

3.       What kind of samples can be detected with Quantibody kits? And how much sample do I need for the experiment?

Quantibody arrays can be used in cell culture supernatant, serum, plasma, CSF, tissue lysate, and other body fluid. Typically, 50-100 μl samples are needed for one array.

We recommend the following parameters for your samples: 50-100 μl of cell culture supernatant, 50-100 μl of original or diluted serum or plasma; or 20-200 μg of protein for cell lysates and tissue lysates. In order to minimize the matrix effects and to lower the background of the assay, we recommended that the samples at least diluted 2 fold with Sample Diluent. Dilute the lysate at least 5 fold with Sample Diluent to make a total volume of 50 to 100 μl. For those samples in which you expect a high response for one cytokine and a low response for another, we recommend testing each sample in step dilutions. For example, when testing cell culture supernatant for unknown cytokine concentrations; a 1:2, a 1:10, and a 1:50 dilution are typically used.

4.      What cytokine standards are used in the Quantibody Array?

Laboratories throughout the world use different bioassays and immunoassays to determine cytokine concentrations. As a result, the reported cytokine concentrations in the same sample may vary when using different ELISAs developed by different labs. For this reason, the availability of international standard preparations of cytokines is essential to allow definitive analysis and comparison of results. Quantibody Arrays use either our internal cytokine standards or the International Biological Standards from the National Institute for Biological Standards and Control (NIBSC), the gold cytokine standards.

 5.      Is there any cross-reactivity between the cytokines?

For the available kits, no. . We have tested all of the available antibodies with their specific purified antigens for the cross-reactivity. The antibody pairs used in the kit have been tested to recognize their specific cytokines. There may be some cross-reactivity between antibodies included in two different kits, but analysis of samples containing only a single recombinant cytokine found no cross-reactivity with other antibodies in the same array. For the custom arrays, we will suggest what cytokines can be included in the same array to ensure the proper level of specificity and sensitivity.

6.      Can I save the remaining cytokine standard for next time use?

The cytokine standards in the kit are provided in the lyophilized mixture form to ensure the stability. For the quality purpose, the cytokine standards once reconstituted should be used in a short period of time (2 –3 days) and can’t be refrozen because they may lose activity during the freeze-thaw cycles. For those in need, we suggest only save the highest concentrated vial, and re-do the serial dilution for a second time usage.

7.      Do I need special software to quantify my data?

Yes and no. After imaging by a laser scanner and data extraction with any of the microarray analysis software (GenePix, ScanArray Express, ArrayVision, or MicroVigene), cytokine quantification can be done with virtually any ELISA analysis software. However, our array-specific Q Analyzer software is available to facilitate the data analysis. Instead of tedious calculation, users can now quickly figure out the unknown sample concentration through a simple copy and paste process. The program can automatically remove the outlier spots for greater accuracy.

8.       I would like to try Quantibody products but I don’t have access to a laser scanner. What can I do?

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Format: Standard glass slide with 16 removable wells Sample Volume: 50-100 -µl Detection Range: 5-3000 pg/ml Standard Curve Range: 5-800 pg/ml Signal Duration: 4 hours Reproducibility: <20% well-to-well CV Detection Method: Fluorescence. Fluorescent dye (Cy3 wavelengths 555 nm excitation, 655 emission)

PRODUCT UNIT CAT #   PRICE Quantibody ® Human Adipokine Array 1 ( IGF-I, IL-1alpha, IL-6, IL-8, Insulin, Leptin, MCP-1, PAI-1, Resistin, TNF alpha) 1 slide QAH-ADI-1-1 $438 2 slides QAH-ADI-1-2 $795 4 slides QAH-ADI-1-4 $1423  Software QAH-ADI-1-SW $180         Quantibody ® Human Adipokine Array 2 (Adipsin, Adiponectin, CRP, SAA) 1 slide QAH-ADI-2-1 $262 2 slides QAH-ADI-2-2 $472 4 slides QAH-ADI-2-4 $846  Software QAH-ADI-2-SW $80         Quantibody® Human MMP Array 1  (MMP-1, MMP-2, MMP-3, MMP-8, MMP-9, MMP-10, MMP-13, TIMP-1, TIMP-2, TIMP-4) 1 slide QAH-MMP-1-1 $438 2 slides QAH-MMP-1-2 $795 4 slides QAH-MMP-1-4 $1423  Software QAH-MMP-1-SW $180         Quantibody® Human Inflammation Array 1  ( IL-1 alpha, IL-1 beta, IL-4, IL-6, IL-8, IL-10, IL-13, MCP-1, IFN-gamma, TNF-alpha) 1 slide QAH-INF-1-1 $438 2 slides QAH-INF-1-2 $795 4 slides QAH-INF-1-4 $1423  Software QAH-INF-1-SW $180         Quantibody® Human Inflammation Array 2 ( IL-1ra, IL-6sR, IL-12p40, IL-12p70, IL-16, OSM, RANTES, SAA, sTNF RI, sTNF RII ) 1 slide QAH-INF-2-1 $438 2 slides QAH-INF-2-2 $795 4 slides QAH-INF-2-4 $1423  Software QAH-INF-2-SW $180         Quantibody® Human Inflammation Array 3 (BLC, Eotaxin, Eotaxin-2, G-CSF, GM-CSF, I-309, ICAM-1, IFNgamma, IL-1alpha, IL-1beta, IL-1ra, IL-2, IL-4, IL-5, IL-6, IL-6 sR, IL-7, IL-8, IL-10, IL-11, IL-12p40, IL-12p70, IL-13, IL-15, IL-16, IL-17, MCP-1, M-CSF, MIG, MIP-1alpha, MIP-1beta, MIP-1delta, PDGF-BB, RANTES, TIMP-1, TIMP-2, TNFalpha, TNFbeta, TNF sRI, TNF sRII) 1 slide QAH-INF-3-1 $1480 2 slides QAH-INF-3-2 $2664 4 slides QAH-INF-3-4 $4780  Software QAH-INF-3-SW $480         Quantibody® Ig Isotype Array ( IgA, IgD, IgE, IgM, IgG1, IgG2, IgG3, IgG4) 1 slide QAH-ISO-1-1 $438 2 slides QAH-ISO-1-2 $795 4 slides QAH-ISO-1-4 $1423  Software QAH-ISO-1-SW $180         Quantibody® Human TH1/TH2 Array 1 ( IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-13, GM-CSF, IFN-gamma, TNF-alpha) 1 slide QAH-TH-1-1 $438 2 slides QAH-TH-1-2 $795 4 slides QAH-TH-1-4 $1423  Software QAH-TH-1-SW $180         Quantibody ® Human Angiogenesis Array 1 ( Angiogenin, ANG-2, EGF, bFGF, HB-EGF, HGF, Leptin, PDGF-BB, PIGF, VEGF) 1 slide QAH-ANG-1-1 $438 2 slides QAH-ANG-1-2 $795 4 slides QAH-ANG-1-4 $1423  Software QAH-ANG-1-SW $180         Quantibody ® Human Angiogenesis Array 2 ( Activin A, AgRP, Angiogenin, ANG-2, ANGPTL4, bFGF, ENA-78, GRO, HB-EGF, HGF, IFNgamma, IGF-I, IL-1alpha, IL-2, IL-6, IL-8, IL-17, IP-10, Leptin, LIF, MCP-1, PDGF-BB, PIGF, RANTES, TGFbeta1, TIMP-1, TIMP-2, TNFalpha, TNFbeta, TPO) 1 slide QAH-ANG-2-1 $1170 2 slides QAH-ANG-2-2 $2106 4 slides QAH-ANG-2-4 $3779  Software QAH-ANG-2-SW $380         Quantibody ® Human Angiogenesis Array 3 ( ANG-1, Angiostatin, CXCL16, EGF, FGF-4, Follistatin, G-CSF, GM-CSF, I-309, IL-1beta, IL-4, IL-10, IL-12p40, IL-12p70, I-TAC, MCP-2, MCP-3, MCP-4, MMP-1, MMP-9, PECAM-1, TGFalpha, TGFbeta3, Tie-1, Tie-2, uPAR, VEGF, VEGF R2, VEGF R3, VEGF-D) 1 slide QAH-ANG-3-1 $1170 2 slides QAH-ANG-3-2 $2106 4 slides QAH-ANG-3-4 $3779  Software QAH-ANG-3-SW $380         Quantibody ® Human Angiogenesis Array 1000( Activin,A, AgRP, ANG-1, ANG-2, Angiogenin, Angiostatin, ANGPTL4, bFGF, CXCL16, EGF, ENA-78, FGF-4, Follistatin, G-CSF, GM-CSF, GRO, HB-EGF, HGF, I-309, IFNgamma, IGF-I, IL-10, IL-12p40, IL-12p70, IL-17, IL-1alpha, IL-1beta, IL-2, IL-4, IL-6, IL-8, IP-10, I-TAC, Leptin, LIF, MCP-1, MCP-2, MCP-3, MCP-4, MMP-1, MMP-9, PDGF-BB, PECAM-1, PIGF, RANTES, TGFalpha, TGFbeta1, TGFbeta3, Tie-1, Tie-2, TIMP-1, TIMP-2, TNFalpha, TNFbeta, TPO, uPAR, VEGF, VEGF,R2, VEGF,R3, VEGF-D) 1 slide QAH-ANG-1000 $2020 Software QAH-ANG-1000-SW $714         Quantibody ® Human Cytokine Antibody Array 1  ( IL-1a, IL-1b, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12, IL-13, GM-CSF, GRO, IFN gamma, MCP-1, MIP-1a, MIP-1b, MMP-9, RANTE, STNFa, VEGF) 1 slide QAH-CYT-1-1 $792 2 slides QAH-CYT-1-2 $1431 4 slides QAH-CYT-1-4 $2561  Software QAH-CYT-1-SW $280         Quantibody ® Human Cytokine Antibody Array 2  ( Acrp30, BDNF, BLC, -NGF, Eotaxin, Eotaxin-2, E-Selectin, GDNF, ICAM-1, IGFBP-1, IGFBP-2, IL-7, IL-17, L-Selectin, M-CSF, MIP-1delta, MIP-3alpha, MIP-3beta, PECAM-1, VCAM-1) 1 slide QAH-CYT-2-1 $792 2 slides QAH-CYT-2-2 $1431 4 slides QAH-CYT-2-4 $2561  Software QAH-CYT-2-SW $280         Quantibody ® Mouse Cytokine Array 1 ( GM-CSF, IFN gamma, IL-1a, IL-1b, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12, IL-13, IL-17, KC, MCP-1, M-CSF, RANTE, STNFa, VEGF) 1 slide QAM-CYT-1-1 $792 2 slides QAM-CYT-1-2 $1431 4 slides QAM-CYT-1-4 $2561  Software QAM-CYT-1-SW $280         Quantibody ® Mouse Cytokine Array 2 (Axl, BLC, CD30, CD40, CXCL16, Eotaxin-2, Fas Ligand, IGFBP-3, IGFBP-5, LIX, L-Selectin, MIG, MIP-1alpha, MIP-1gamma, PF-4, P-Selectin, SDF-1alpha, TCA-3, sTNF RII, VCAM-1) 1 slide QAM-CYT-2-1 $792 2 slides QAM-CYT-2-2 $1431 4 slides QAM-CYT-2-4 $2561  Software QAM-CYT-2-SW $280         Quantibody ® Mouse Cytokine Array 3 (Eotaxin, E-Selectin, G-CSF, GITR, ICAM-1, IGFBP-2, IGFBP-6, IGF-I, IL-12p40p70, Leptin, MCP-5, MDC, MIP-2, MIP-3alpha, Osteopontin, OPG, Resistin, SCF, TPO, VEGF-D) 1 slide QAM-CYT-3-1 $792 2 slides QAM-CYT-3-2 $1431 4 slides QAM-CYT-3-4 $2561  Software QAM-CYT-3-SW $280         Quantibody ® Mouse Cytokine Array Q2000 (Combination of Mouse Cytokine Antibody Array 1, 2, 3 - Detect 60 proteins in one experiment) 1 slide QAM-CYT-Q2000-1 $2020 2 slides QAM-CYT-Q2000-2 $3650 4 slides QAM-CYT-Q2000-4 $6530  Software QAM-CYT-Q2000-SW $714         Quantibody ® Mouse Inflammation Array 1 ( BLC, CD30L, Eotaxin, Eotaxin-2, Fas L, G-CSF, GM-CSF, ICAM-1, IFN-gamma, IL-1-alpha, IL-1-beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-10, IL-12p70, IL-13, IL-15, IL-17, IL-21, KC, Leptin, LIX, MCP-1, MCP-5, MCSF, MIG, MIP-1 alpha, MIP-1 delta, PF-4, RANTES, TARC, TCA-3, TIMP-1, TNF alpha, TNFRI, TNFRII) 1 slide QAM-INF-1-1 $1480 2 slides QAM-INF-1-2 $2664 4 slides QAM-INF-1-4 $4780  Software QAM-INF-1-SW $480         Quantibody® Rat Antibody Array 1 ( CINC-2, CINC-3, CNTF, Fractalkine, GM-CSF, IL-1a, IL-4, LIX, bNGF, VEGF) 1 slide QAR-CYT-1-1