RTK Phosphorylation Array

Semi-quantitative, Sandwich-based Array for Detecting RTK Phosphorylation Signatures

The RayBio® Human RTK Phosphorylation Antibody Array is a rapid, convenient and sensitive assay for simultaneous detection of phosphorylation of 71 different human tyrosine kinase receptors (RTKs).  This assay allows the researcher to monitor pathway activation in cells or tissues without the excess time and effort of immunoprecipitation or Western blot.

The Human RTK Phosphorylation Array is spotted with a panel of 71 RTK-specific antibodies to capture the tyrosine kinases of interest.  A biotinylated anti-phosphotyrosine antibody is then utilized to detect relative levels of activation in each captured protein.  The array signals can be developed by chemiluminescence (membrane-based format), or fluorescence (glass slide-based format).  

For a comparison & overview of all phosphorylation assays, click here.

Contents of kit

• Detect activation of 71 tyrosine kinases in a single experiment
• Minimal hands-on time: convenient, semi-quantitative
• No immunoprecipitations, Western blots, or radioactive materials needed
• No special equipment required
• Chemiluminescent or fluorescent readout
• Work reliably with wide range of cell lines 
• High sensitivity and specificity
• Affordable and cost effective for routine use

• Antibody array membranes or glass slides
• 2X Cell Lysis Buffer
• Protease Inhibitor Cocktail
• Phosphatase Inhibitor Cocktail
• Blocking Buffer 
• Wash Buffer 1
• Wash Buffer 2
• Biotin-Conjugated Anti-Phosphotyrosine 
• Streptavidin-Conjugated HRP or fluor
• Detection Buffers (membrane array kits only)

After a blocking step, treated or untreated cell lysate is added into antibody array (either membrane or glass slide). The arrays are washed and biotinylated anti-phosphotyrosine antibody is added. After incubation with streptavidin-conjugated HRP or fluor, the signals are then visualized by chemiluminescence or fluorescence imaging system.

For a list of laser scanner specifications, click here.

If you do not have access to a scanner, RayBiotech provides slide scanning and data analysis services.