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Ultrasensitive Biomarker Testing Service

1,000 Times More Sensitive Than ELISA

Single molecule array (Simoa™) testing is a powerful new technique that is orders of magnitude more sensitive than standard sandwich based immunoassay techniques. Traditional ELISA measurements are limited to pg/ml levels of detection. The RayBio Ultrasensitive Simoa Testing Services can achieve sensitivity as low as femtogram (fg/ml) levels, allowing the detection and quantification of biomarkers at concentrations previously difficult or impossible to measure.

 

Available Assays

RayBiotech is currently offering the below ultrasensitive human assays for immediate testing:

Amyloid beta 1-40
C-Peptide
Cardiac Troponin I
(cTnI)
Eotaxin-1
(CCL11)
GCSF
GM-CSF
IFN alpha
IL-1 beta
(IL-1 F2)
IL-10
IL-12 p70
IL-13
IL-15
IL-16
IL-17A
IL-17A
IL-2
IL-23
IL-5
IL-6
IL-7
IL-8
(CXCL8)
IP-10
(CXCL10)
MCP-1
(CCL2)
p24
  • Gene Symbol: TMED2 RNP24
  • Gene ID: 10959
  • Accession #: Q15363
PSA-free
MAPT
(Tau)
TNF alpha
TRAIL
(TNFSF10)
Troponin I
 

Don't see the biomarker you're looking for? The entire RayBiotech immunoassay menu (>1,600 targets covering 12 different species) is available for conversion to the Simoa testing service platform. Simply request the biomarker you are interested in and we will develop the Simoa for it. Please note there is an additional 4-6 week lead time.

View the Complete List of Biomarkers Available

 

To receive a quote*, fill out the sample service form linked below and email it to orders@raybiotech.com.

*10 sample minimum

Sample Service Form      Sample Shipping Instructions

 

How It Works

Simoa is based upon the isolation of individual immunocomplexes on paramagnetic beads using standard ELISA reagents. The main difference between Simoa and conventional immunoassays lies in the ability to trap single molecules in femtoliter-sized wells, allowing for a “digital” readout of each individual bead to determine if it is bound to the target analyte or not.

Simoa Sensitivity Comparison

 

Representative Data

Ultrasensitive IL-6 assay

 
IL-6 Multiday Testing

 

Day 1

Day 2

Day 3

Mean

CV

Serum 1

2.8845

2.8332

2.7788

2.8322

1.9%

Serum 2

1.8079

1.8785

1.8792

1.8552

2.2%

Serum 3

2.2035

2.1724

2.2078

2.1946

0.9%

 

References

Single Molecule Arrays (Simoa) for the Ultra-Sensitive Detection of the Host Immune Response to Dengue Infection
February 18, 2015
Shonda T. Gaylord, Sama Abdul-Aziz, David R. Walt
J. Clin. Microbiol., February 18, 2015
doi:10.1128/JCM.03487-14

 

Emerging Technologies to Increase Ligand Binding Assay Sensitivity
January 2015
Saloumeh K. Fischer et. al.
The AAPS Journal, Vol. 17, No. 1, January 2015
DOI: 10.1208/s12248-014-9682-8

 

Anew ultra-high sensitivity troponin I assay for chest pain patients with no evidence of troponin I using a conventional assay
December 4, 2014
Wu AHB, van Wijk XMR
Clin Biochem 2014
http://dx.doi.org/10.1016/j.clinbiochem.2014.11.018

 

Next Generation Ligand Binding Assays—Review of Emerging Technologies’ Capabilities to Enhance Throughput and Multiplexing
November 2014
Johanna Mora et al
The AAPS Journal, Vol. 16, No. 6, November 2014
DOI: 10.1208/s12248-014-9660-1

 

High Sensitivity Cardiac Troponin Assays in the Clinical Laboratories
October 22, 2014
Petr Jarolim
Clin Chem Lab Med 2014
DOI 10.1515/cclm-2014-0565

 

CSF and Plasma Amyloid-b Temporal Profiles and Relationships with Neurological Status and Mortality after Severe Traumatic Brain Injury
October 10, 2014
by Stefania Mondello, Andras Burk, Pal Barzo, Jeff Randall, Gail Provuncher, David Hanlon, David Wilson, Firas Kobeissy & Andreas Jeromin

 

BloodBiomarkers for Brain Injury in Concussed Professional Ice Hockey Players
March 13, 2014
JAMA Neurology. Published online March 13, 2014. doi:10.1001/jamaneurol.2014.367
Pashtun Shahim, MD; Yelverton Tegner, MD, PhD; David H. Wilson, PhD; Jeffrey Randall, PhD3; Tobias Skillbäck, MD; David Pazooki, MD, PhD; Birgitta Kallberg, BSc; Kaj Blennow, MD, PhD; Henrik Zetterberg, MD, PhD

 

Tau proteins in serum predict neurological outcome after hypoxic brain injury from cardiac arrest: Results of a pilot study
March 2013
Resuscitation Journal
J, Mortberg E, Provuncher G, Fournier D, Duffy D, Rubertsson S, Blennow K, Zetterberg H, Wilson D.

 

Plasma tau levels in Alzheimer's disease
March 28, 2013
Alzheimer's Research & Therapy
Henrik Zetterberg, David Wilson, Ulf Andreasson, Lennart Minthon, Kaj Blennow, Jeffrey Randall and Oskar Hansson

 

Olympic boxing is associated with elevated levels of the neuronal protein tau in plasma
March 8, 2013
Brain Injury
Neselius S, Zetterberg H, Blennow K, Randall J, Wilson D, Marcusson J, Brisby H.

 

Single molecule enzyme-linked immunosorbent assays: Theoretical considerations
Lei Chang, David M. Rissin, David R. Fournier, Tomasz Piech, Purvish P. Patel, David H. Wilson, David C. Duffy. Journal of Immunological Methods 378 (2012) pp. 102-115

 

Single-molecule enzyme-linked immunosorbent assay detects serum proteins at subfemtomolar concentrations
David M Rissin, Cheuk W Kan, Todd G Campbell, Stuart C Howes, David R Fournier, Linan Song, Tomasz Piech, Purvish P Patel, Lei Chang, Andrew J Rivnak, Evan P Ferrell, Jeffrey D Randall, Gail K Provuncher, David R Walt & David C Duffy
Nature Biotechnology 28, 595–599 (May 2010) doi:10.1038/nbt.1641

 

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