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Western Blotting Just Got Easier
- Had enough of running gels and transfers?
- Hate the thought of buying and testing another antibody?
- Have a lot of samples & very little time?
- Have < 20 µl sample?
Leave the burden of validating antibodies and optimizing conditions to us. RayBiotech’s Auto-Western Service marries our state-of-the-art capillary immunoblotting system with our vast pre-validated antibody library. Our high throughput system can achieve ng to pg sensitivity with only 5 µL of starting material. With a library of over 2,000 antibodies, all you need to do is send us your samples and we’ll do the rest. If we don't have your antibody of choice in stock you can even send us your own antibody.
- Low sample volume required (5 µl)
- As low as 0.2 µg/µl protein
- Ng to pg sensitivity
- Full analysis report included (see example below)
- Standard curve may be included for full quantification (additional charges apply)
Get a Quote Today!
For as little as $30 per sample*, you will receive a data report with signal peak diagrams and a digitized virtual gel image. To obtain a service quotation, simply fill out a quote request form linked below or email our ordering department (firstname.lastname@example.org). They will email you back a formal quote as soon as possible. Above pricing for North America customers only.
*for in stock antibodies. Purchasing fees may apply for antibodies not in stock
Results are presented in electropherogram format (A) with each peak displayed numerically (B). Electropherogram peaks are then digitally rendered as a “virtual blot” (C).
How It Works
RayBiotech's western blot service uses a highly accurate and precise capillary immunoassay method. Samples and reagents are loaded into an assay plate and placed in our western machine. As little as 40 nL of sample is loaded into the capillary automatically and separated by size as they migrate through a stacking and separation matrix. The separated proteins are then immobilized to the capillary wall via a proprietary, photoactivated capture chemistry. Target proteins are identified using a primary antibody and immunoprobed using an HRP-conjugated secondary antibody and chemiluminescent substrate. The resulting chemiluminescent signal is detected and quantitated. (image produced by ProteinSimple)
Quality Control Testing Data
RayBiotech’s Auto-Western platform exhibits linear signal response down to the picogram (pg) range.