Mouse anti-Human C4d Antibody

Mouse anti-Human C4d Antibody

$198.00
188-10728-1
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Antigen Information

Accession Number:
  • P0C0L4,P0C0L5
Gene ID:
  • 720
  • 721
  • 100293534
Gene Symbols:
  • C4A
  • C4B
  • C4B_2
  • CO4
  • CPAMD2
  • CPAMD3
Protein Name & Synonyms:
Complement C4-A (Acidic complement C4) (C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2) [Cleaved into: Complement C4 beta chain, Complement C4-A alpha chain, C4a anaphylatoxin, C4b-A, C4d-A, Complement C4 gamma chain], Complement C4-B (Basic complement C4) (C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3) [Cleaved into: Complement C4 beta chain, Complement C4-B alpha chain, C4a anaphylatoxin, C4b-B, C4d-B, Complement C4 gamma chain]
Target Species:
  • Human

Antibody Specifications

Applications:
ELISA: 1-2ug/ml (order Ab without BSA)
Immunofluorescence: 0.5-1ug/ml
Concentration:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Domain/Region/Terminus:
Intracytoplasmic vacuoles of endothelial cells and secreted
Host Species:
Mouse
Preparation:
Human Complement 4d protein was used as the immunogen for the C4d antibody.
Purity:
Protein G affinity chromatography
Species Reactivity:
Human. Other species not tested.

Description

This mAb is specific to Complement 4d (C4d) and it reacts with the secreted as well as cell-bound C4d. C4d is a degradation product of the activated complement factor C4b. Complement 4b is typically activated by binding of Abs to specific target molecules. Following activation and degradation of the C4 molecule, thio-ester groups are exposed, which allow transient, covalent binding of the degradation product Complement 4d to endothelial cell surfaces and extracellular matrix components of vascular basement membranes near the sites of C4 activation. The presence of C4d in peritubular capillaries is a key indicator for acute humoral (i.e. antibody-mediated) rejection of kidney, heart, pancreas and lung allografts. As an established marker of antibody-mediated acute renal allograft rejection and its proclivity for endothelium, this component can be detected in peritubular capillaries in chronic renal allograft rejection as well as hyperacute rejection, acute vascular rejection, acute cellular rejection, and borderline rejection. It has been shown to be a significant predictor of transplant kidney graft survival. Anti-C4d, combined with anti-C3d, can be utilized as a tool for diagnosis of allograft rejection that may warrant a prompt and aggressive anti-rejection treatment.

Storage

The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.