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Human PCSK2 ELISA

$462.00
ELH-PCSK2-1

RayBio® Human PCSK2 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

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Product Features

Sensitivity
2.45 ng/ml

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Detection Range
2.458 ng/ml - 600 ng/ml
Recommended Dilution (serum & plasma)
2 fold
Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Specificity

This ELISA antibody pair detects human PCSK2. Other species not determined.
Species Detected
Human
Protein Name & Synonyms
Neuroendocrine convertase 2 (NEC 2) (EC 3.4.21.94) (KEX2-like endoprotease 2) (Prohormone convertase 2) (Proprotein convertase 2) (PC2)
Accession Number
Gene Names
PCSK2 NEC2
Gene ID
5126

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 ?l to 1 ?l volumes
  • Adjustable 1-25 ?l pipettes for reagent preparation
  • 100 ?l and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 ?l of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4?C.
  4. Add 100 ?l of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 ?l of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 ?l of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 ?l of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20?C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4?C for up to 6 months. For extended storage, it is recommended to store at -80?C.

Antigen Information

Gene Symbol:
  • PCSK2

Array/ELISA Format

Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Serum
  • Plasma
  • Cell Culture Supernatants
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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