Recombinant Human Apolipoprotein E (Apo E)

Recombinant human Apolipoprotein E, derived from the transfected human HEK293 cells.

230-30049-10
+

Source

Gene Symbols:
  • APOE
Species:
Human
Accession Number:
  • P02649
Gene ID:
  • 348
Expressed Region:
Lys19-His317
Protein Name & Synonyms:
Apolipoprotein E (Apo-E), Apolipoprotein E, APO-E, Alzheimer Disease 2 (APOE*E4-Associated, Late Onset), Apolipoprotein E3, LDLCQ5, ApoE4, LPG, AD2

Preparation

Expression System:
Human Embryonic Kidney 293 Cells
Conjugation/Tag:
N-terminal histidine tag
Purification:
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Purity:
>95%
Purity determined by:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa):
Recombinant protein has a calculated molecular weight of about 37 kDa.

Protein Specifications

Format:
Lyophilized powder
Formulation:
Lyophilized from a 0.2 µm filtered solution in PBS
Concentration:
Determined by Pierce BCA protein assay kit
Reconstitution:
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1 mg/mL with sterile deionized water.
Endotoxin Level:
Not determined
Preservative:
None
SDS-PAGE Image

Stability & Storage

Shipping
Ice packs
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20 °C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 °C.  For long term storage, it is recommended to make appropriate aliquots and store at -80 °C. Avoid repeated freeze-thaw cycles.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.