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Akt Activity Assay Kit, KinaseSTAR

40 assays

$693.00  
68AT-Akt-S40
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Antigen Information

Target Species:
Mammalian

Assay Format

Compatible Sample Types:
  • Cell Lysates
  • Tissue Lysates
Method of Detection:
Chemiluminescence

Product Specifications

Size:
40 assays

Product Feature

  • Simple procedure
  • The kit is suitable for both research and drug discovery
  • The kit specifically detects Akt1, Akt2, and Akt3 activities, other kinase activities would not be detected.

Species Detected

Mammalian

Summary

  • Detection method- Western blot analysis using anti-Phospho-GSK-3α (Ser 21) antibody at 1:1000 dilutions
  • Species reactivity- Mammalian
  • Applications- The Akt Activity Assay Kit utilizes an Akt-specific antibody to immunoprecipitate Akt from cell lysate. Activity of the Akt is then determined in a kinase reaction using recombinant GSK-3α as substrate.
Kit Components
  • Kinase Extraction Buffer
  • Akt Specific Antibody
  • Protein A Sepharose
  • GSK-3 Protein/ATP Mixture
  • Kinase Assay Buffer
  • Phospho-GSK-3α Specific Antibody

Storage/Stability

-20°C

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.