The kit is suitable for both research and drug discovery
The kit specifically detects Akt1, Akt2, and Akt3 activities, other kinase activities would not be detected.
Detection method- Western blot analysis using anti-Phospho-GSK-3α (Ser 21) antibody at 1:1000 dilutions
Species reactivity- Mammalian
Applications- The Akt Activity Assay Kit utilizes an Akt-specific antibody to immunoprecipitate Akt from cell lysate. Activity of the Akt is then determined in a kinase reaction using recombinant GSK-3α as substrate.