Allergen Protein Arrays

Introduction

The RayBio^® Allergen Protein Arrays are semi-quantitative multiplex protein arrays developed for the detection of antibodies to common allergens. Recombinant allergen proteins or extracted native allergen proteins are first spotted onto a solid glass slide surface, and then probed with serum to measure the presence and levels of serum-specific IgE and/or IgG antibodies targeting these allergens.

For a comparison & overview of all our protein array systems, click here.

Allergen Protein Array Features

Get 3x the data, plus high throughput

Each 75mm x 25mm glass slide is spotted with 16 identical protein arrays (also called "subarrays"). Within each subarray, the allergens along with controls are spotted in triplicate. The slide comes with a 16-well removable gasket and chamber assembly that allows for simultaneous processing of up to 16 samples per slide. To achieve even higher throughput, 4 slides can be nested into a tray which matches a standard microplate, allowing for automated processing of 64 arrays simultaneously. The latest automated liquid handling work stations can accommodate several trays at once, allowing hundreds of samples to be processed per day.

How It Works

A panel of allergen proteins is printed in multiple identical arrays on a standard slide. After a blocking step, samples are incubated with the arrays. Nonspecific antibodies are then washed off, and the arrays are incubated with Biotin-conjugated anti-human IgG and/or IgE detection antibodies, followed by a streptavidin-conjugated fluor. Signals are then visualized using a fluorescence laser scanner.

For a list of laser scanner specifications, click here.

Testing Service

Full Testing Service: Ship us your samples (5 sample minimum) and our experienced service department will run the array and analyze the data for you. Email our tech support team at [email protected] to learn more.

Scanning Service: If you do not have access to a scanner, RayBiotech provides slide scanning and data analysis services

RayBiotech Protein Array slide scanning and data extraction service is now FREE for a limited time only! Simply ship us your slide/s and we will scan the slide/s and send you the raw data extracted from the image. Click to learn more

Need help analyzing all that data? Copy and paste your data into the RayBiotech Analysis Tool specific for your array. The analysis will automatically perform background subtraction and normalization.

Research Applications

• High-throughput profiling of allergens in human serum
• Identifying potential allergens based on IgE and IgG levels

Human Allergen Protein Array G2

Contains 22 common allergen proteins:

Human Allergen Protein Array G3

Contains 82 common allergen proteins: