Research Antibodies

With all antibodies manufactured on-site in our Peachtree, GA, facilities, you can be confident that every one of the >18,000 monoclonal and polyclonal research antibodies we offer meets our stringent quality requirements.

Detect, Measure, And Discover More With Antibody Reagents From RayBiotech

Because your data is only as good as the reagents that go into generating it, you need to be confident in the quality and consistency of the materials you use. This is why we insist on manufacturing everything we sell—it’s the best way to ensure that our antibody reagents will support the success of your studies, enabling you to detect, measure, and discover more.

Antibody Categories

Primary Antibodies

Move quickly with ready-to-use primary antibodies that target a wide range of proteins from commonly-used species.

Secondary Antibodies

Find a secondary antibody that enables visualization of your primary antibody using the best detection method for your studies.

Flow Cytometry Antibodies

Choose from a selection of antibodies validated for flow cytometry applications and conjugated to RayBright® dyes.

Isotype Controls

Confidently differentiate non-specific background signal from specific antibody signal with isotope controls.

Popular Research Antibodies

New Products

What customers are saying

Excellent antibody [Rat Anti-Mouse IL-2, Cat# RB-IP-01-102], nice peak of positive population.

Rahul

Suchita

Salk Institute

3d rendering of antibody Immunoglobulin molecule background.

Featured Services

Don’t Limit Yourself to What’s On the Shelf.

RayBiotech offers a custom research antibody service covering every step of monoclonal and polyclonal development.

Our capabilities include peptide antigen design (including phospho peptides), synthesis and carrier protein conjugation, immunization, titer analysis, serum collection or hybridoma fusion, and antibody purification. Customization options are available, such as antibody conjugation, lyophilization, custom vialing & aliquoting, and antibody validation by Western blot, ELISA, or immunoprecipitation.

Tips for choosing a research antibody

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1. Review the literature

Read studies where researchers detect the exact targets you're interested in. Then, note the products they used, the protocols they followed, and the applications that worked for them.

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2. Choose an antibody for your detection method

Antibodies that work in one application don't necessarily work in a different application. For example, an antibody that worked in a Western Blot may not be suitable for use in IHC.

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3. Ensure the host species supports your target

To avoid cross-reactivity, the host species of your primary should be different from the host species of your sample. Consider your challenges and goals and consciously select a monoclonal or polyclonal antibody.

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4. Select an appropriate secondary antibody

Choose a secondary antibody raised against your primary antibody's host species. Just like when choosing a primary antibody, consider the application.

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5. Read the product datasheet

Product web pages and datasheets can be a constructive way of gathering information and making the right decision for your research. Read these thoroughly.

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6. Study the protocol

Immunoassay protocols require optimization. To save yourself a lot of time and money, make sure to follow the manufacturer's instructions.

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7. Store and handle the antibody appropriately

Nothing is more frustrating than throwing away an unused product ruined by improper storage. Follow the instructions to best reconstitute, store, and aliquot your antibodies.

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8. Include experimental controls

Proper data interpretation relies on the inclusion of positive and negative controls. Controls ensure antibodies are behaving as expected and help distinguish background signal.

Frequently Asked Questions

Still have questions?

Primary and secondary antibodies differ in how they bind to antigens.

Primary antibodies bind directly to antigens in a tissue or sample, whereas secondary antibodies bind to primary antibodies creating an antigen-primary-secondary chain.

Direct immunoassays use primary antibodies to detect antigens. Indirect immunoassays use both primary and secondary antibodies.

An isotype control is a primary antibody that lacks specificity against the target antigen but matches the type and class of the primary antibody used in the experiment.

They serve as negative controls that generate reliable data by allowing researchers to accurately differentiate between specific antibody signal and non-specific interactions causing background signal.

Antibodies for flow cytometry allow scientists to detect specific antigens and characterize the proteins on the surface of cells. Similar to other immunoassays, antibodies used in flow cytometry can be directly labeled with a fluorescent dye or bind to a secondary antibody carrying the fluorescent marker. The specialized nature of antibodies enables flow cytometry to detect different cell types according to their fluorescent color, each representing an antigen-antibody interaction.

Antibodies are created by inoculating an animal with an antigen and harvesting antibodies from blood serum.

Choose a primary antibody that was produced in a host species that is different from the species that provided your sample. For example, if you are studying mouse samples, choose a primary that was not raised in mouse.

Find a secondary antibody that is against the host species of your primary antibody. For example, if the primary antibody was raised in mouse, choose an anti-mouse secondary.

Still have questions?

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