Antibody Arrays: Methods and Protocols

In the series: Methods in Molecular Biology

Part of Springer Nature

Editors: Kelly C. Whittaker and Ruo-Pan Huang

High-throughput methodologies to investigate protein function have become an essential tool for understanding the molecular basis of disease and to generate meaningful data that informs treatment decisions. The complexity of the molecular landscape of disease, along with persistent efforts by the research community to improve precision medicine, has driven significant advancements in antibody array technology. Over the past 20 years, antibody arrays have been used extensively to elucidate the molecular basis of cancer, autoimmunity, neurodegeneration, and numerous other disease processes in a variety of biological samples as well as identify target proteins for therapeutic intervention and discover biomarkers for the early diagnosis or prognosis of disease.

This book Antibody Arrays: Methods and Protocols, written for the highly successful Methods in Molecular Biology series, presents a technical overview and practical methodology of a variety of antibody array formats and technologies. Practical applications of the antibody arrays to specific research topics and biological processes are included to help guide users in selecting the most appropriate array for their experimental design. In addition, guidance on the step-by-step methods of processing, analysis, and storage of array data are explored – to ensure the end user has the tools to perform the assay and acquire the maximum usefulness of the generated data.

Antibody Arrays: Methods and Protocols book cover

Chapter List

  1. Sandwich-Based Antibody Arrays for Protein Detection
  2. Well-Based Antibody Arrays
  3. One-Step Antibody Arrays
  4. Global Detection of Proteins by Label-Based Antibody Array
  5. Surface Plasmon Resonance Imaging-Mass Spectrometry Coupling on Antibody Array Biochip: Multiplex Monitoring of Biomolecular Interactions and On-Chip Identification of Captured Antigen
  6. Multiplexed Protein Biomarker Detection with Microfluidic Electrochemical Immunoarrays
  7. Cytometry Multiplex Bead Antibody Array
  8. Antibody Arrays: Barcode Technology
  9. Reverse Phase Protein Arrays
  10. Enhanced Protein Profiling Arrays for High-Throughput Quantitative Measurement of Cytokine Expression
  11. Methods for Membranes and Other Absorbent Surfaces
  12. Precise Chip-to-Chip Reagent Transfer for Cross-Reactivity-Free Multiplex Sandwich Immunoassays
  13. Antibody Printing Technologies
  14. Self-Assembling Peptide Hydrogels for 3D Microarrays
  15. Surface Modification of Glass Slides with Aminosilanes for Microarray Use
  16. Dried Blood-Based Protein Profiling Using Antibody Arrays
  17. Profiling Glycoproteins on Functionalized Reverse Phase Protein Array
  18. Determining Protein Phosphorylation Status Using Antibody Arrays and Phos-Tag Biotin
  19. Analyzing Signaling Pathways Using Antibody Arrays
  20. Using Antibody Arrays for Biomarker Discovery
  21. Immune Cell Isolation from Murine Intestine for Antibody Array Analysis
  22. Database Development for Antibody Arrays
  23. Data Analysis for Antibody Arrays
  24. Statistical Analysis Options for Antibody Array Data