Antibody Arrays: Methods and Protocols

In the series: Methods in Molecular Biology

Part of Springer Nature

Editors: Kelly C. Whittaker and Ruo-Pan Huang

High-throughput methodologies to investigate protein function have become an essential tool for understanding the molecular basis of disease and to generate meaningful data that informs treatment decisions. The complexity of the molecular landscape of disease, along with persistent efforts by the research community to improve precision medicine, has driven significant advancements in antibody array technology. Over the past 20 years, antibody arrays have been used extensively to elucidate the molecular basis of cancer, autoimmunity, neurodegeneration, and numerous other disease processes in a variety of biological samples as well as identify target proteins for therapeutic intervention and discover biomarkers for the early diagnosis or prognosis of disease.

This book Antibody Arrays: Methods and Protocols, written for the highly successful Methods in Molecular Biology series, presents a technical overview and practical methodology of a variety of antibody array formats and technologies. Practical applications of the antibody arrays to specific research topics and biological processes are included to help guide users in selecting the most appropriate array for their experimental design. In addition, guidance on the step-by-step methods of processing, analysis, and storage of array data are explored – to ensure the end user has the tools to perform the assay and acquire the maximum usefulness of the generated data.