Antibody Services
Leverage the same quality antibody production processes we put into our products for your custom research and bulk antibody production needs.
Our custom antibody services cover every step of the monoclonal and polyclonal development process and can include peptide antigen design (including phospho peptides), synthesis and carrier protein conjugation, immunization, titer analysis, serum collection or hybridoma fusion, and antibody purification.
We also offer a number of customizations, including antibody conjugation, lyophilization, custom vialing and aliquoting, and antibody validation by western blot, ELISA, or immunoprecipitation.
Why You Should Choose Our Antibody Services
For Our Years of Experience
Founded in 2001 as the first company to commercialize the antibody array, we continue to use our decades of experience in multiplex, high-throughput protein analysis to deliver outstanding protein analysis and antibody-related services to the life science community.
What you can expect
| Polyclonal Versus Monoclonal Antibody Production At-A-Glance | ||
|---|---|---|
| Polyclonal | Monoclonal | |
| Production time | ~2 Months | 4-6 Months |
| Animal use | Yes | Yes |
| Antibody size | 150 - 900 kDa | 150 kDa |
| Avidity/Affinity | High (Avidity) | Variable (Affinity) |
| Binding sites recognized | Multiple | Single |
| Can be reproducibly produced | No | Yes |
Antibody purification and conjugation
Our antibody purification and conjugation service typically takes 2-3 days and can include the conjugation of polyclonal or monoclonal antibodies to enzymes, fluorochromes, DNA, or affinity ligands as well as to solid supports.
Polyclonal production options and timeline
Polyclonals raised against a peptide
Option 1: Customer-provided peptide
Production time: 5 weeks
Amount: 10 mg purified antibody
Process:
You
- Send us 1 mg non-conjugated or 2 mg KLH-conjugated (90% purity) peptide
RayBiotech
- Immunizes two rabbits (other species available)
- Performs test bleeds, titer tests
- Purifies serum by protein A/G column
- Performs antigen affinity purification if requested
Option 2: RayBiotech synthesizes the peptide
Production time: 8 weeks
Amount: 10 mg purified antibody
Process:
RayBiotech
- Designs, synthesizes and conjugates the peptide antigen (15-20-mer, 5-10 mg)
- Immunizes two rabbits (other species available)
- Performs test bleeds, titer tests
- Purifies serum by protein A/G column
- Performs antigen affinity purification if requested
Polyclonals raised against a phospho-peptide
Production time: 8-10 weeks
Amount: 10 mg purified antibody
Process:
RayBiotech
- Designs, synthesizes and conjugates the phosphorylated peptide antigen (15-20-mer, 5-10 mg)
- Immunizes two rabbits (other species available)
- Performs test bleeds, titer tests
- Purifies serum by protein A/G column
- Performs antigen affinity purification if requested
Polyclonals raised against a protein
Production time: 5-7 weeks
Amount: 10 mg purified antibody
Process:
You
- Send us 5 mg of protein antigen
RayBiotech
- Immunizes two rabbits (other species available)
- Performs test bleeds, titer tests
- Purifies serum by protein A/G column
- Performs antigen affinity purification if requested
Monoclonal production options and timeline
Monoclonals raised against a peptide
Option 1: Customer-provided peptide
Production time: 4-5 months
Process:
You
- Send us 4 mg of peptide antigen
RayBiotech
- Immunizes up to five mice and performs fusion, screening, subcloning, and initial characterization
- Identifies up to three monoclones and their supernatant to confirm detection
- Verifies immunogen recognition using an ELISA
- Purifies monoclonal from 100 mL of hybridoma culture supernatant or 1 mL of ascites fluid
Option 2: RayBiotech synthesizes the peptide
Production time: 4-5 months
Process:
RayBiotech
- Designs, synthesizes and conjugates the peptide antigen (15-20-mer, 5-10 mg)
- Immunizes up to five mice and performs fusion, screening, subcloning, and initial characterization
- Identifies up to three monoclones and their supernatant to confirm detection
- Verifies immunogen recognition using an ELISA
- Purifies monoclonal from 100 mL of hybridoma culture supernatant or 1 mL of ascites fluid
Monoclonals raised against a phospho-peptide
Production time: 5-6 months
Process:
RayBiotech
- Designs, synthesizes and conjugates the peptide antigen (15-20-mer, 5-10 mg)
- Immunizes up to five mice and performs fusion, screening, subcloning, and initial characterization
- Identifies up to three monoclones and their supernatant to confirm detection
- Verifies immunogen recognition using an ELISA
- Purifies monoclonal from 100 mL of hybridoma culture supernatant or 1 mL of ascites fluid
Monoclonals raised against a protein
Production time: 5 months
Process:
You
- Send us 4 mg of purified protein antigen
RayBiotech
- Immunizes up to five mice and performs fusion, screening, subcloning, and initial characterization
- Identifies up to three monoclones and their supernatant to confirm detection
- Verifies immunogen recognition using an ELISA
- Purifies monoclonal from 100 mL of hybridoma culture supernatant or 1 mL of ascites fluid
Ready for RayBiotech to start working on your Antibody Service?
Frequently Asked Questions
Still have questions?
We offer a diverse selection of over 18,000 monoclonal and polyclonal primary antibodies. RayBiotech's antibodies were developed to target multiple different proteins relevant to cell biology, neurobiology, immunology, virology, and many other fields. We’ve also developed secondary antibodies that target multiple host species immunoglobulin isotypes. These are available unlabeled or pre-conjugated to many different reporter systems, including alkaline phosphatase, biotin, FITC and other labels.
We can usually raise polyclonal antibodies against 150 to 900 kDa antigens in about 2 months. These wil be high affinity, low specificity antibodies, capable of binding multiple sites. Monoclonal antibodies can be raised against proteins no larger than 150 kDa in 4 to 6 months. These antibodies will be highly specific for only one binding site and their production can be scaled.
Still have questions?
Featured Resources
Get additional information to help you advance your studies from articles on the science underlying antibody-based techniques to video protocols, application notes, and more.
