Large Scale Antibody Production
RayBiotech provides large scale antibody production and purification services of monoclonal antibodies from pre-existing hybridoma cell lines:
- Scalable production
- Serum-free culture media - no contamination of bovine antibodies
- Endotoxin testing - less than 1 EU/mg guaranteed!
- Carrier-free - great for animal studies
- Protein A, G, or L affinity purification
- Discounts for academic and government institutions
Service Phases
1: Pilot Antibody Production
Description
The primary objective of this phase is to determine how well the hybridoma cell line produces antibodies.
The hybridoma cell line is cultured and propagated in serum-free media.
Antibody is purified and the amount determined via a BCA assay.
Lead time
2 - 4 weeks
Deliverable
The concentration of the purified antibody concentration is provided via email. Purified antibodies can be shipped to customers immediately or kept until Phase 3 is complete.
The amount of purified antibody following this step is not guaranteed as the level of antibody production varies. Based upon the needs of the customer, the cost and lead times for Phases 2 - 3 can now be accurately provided.
2: Hybridoma Expansion
Description
The hybridoma cell line is cultured and propagated in serum-free media.
Lead time
Depends on hybridoma
Deliverable
No deliverable. Service will immediately continue to Phase 3.
3: Antibody Purification
Description
This phase includes the following steps:
Purification using Protein A, G, or L affinity chromatography
Antibody concentration determined using a BCA assay
Endotoxin testing: < 1 EU/mg is guaranteed
Sterilization (optional)
Dilution to desired antibody concentration (optional)
Aliquoting (optional)
Lead time
1 - 2 weeks
Deliverable
Data will be sent electronically to the customer. Purified antibodies will be shipped to the customer.
