Angiogenesis

Tools to elucidate molecular mechanisms

Blood vessel growth, a process known as angiogenesis, is essential for normal tissue growth, development, wound healing and repair. The formation of new blood vessels is a complex process requiring a tightly regulated balance of both stimulatory and inhibitory signals. Dysregulation of angiogenesis causes numerous pathologies, including (but not limited to) immune, inflammatory, ischemic, or malignant disease states. Common examples of disease arising from excessive angiogenesis include cancer, psoriasis, arthritis, and even obesity. On the other hand, insufficient blood vessel growth may culminate in neurodegeneration, ischemia, hypertension, or osteoporosis. As pathological angiogenesis is recognized as an underlying mechanism for an ever-increasing list of diseases, pharmacologic agents have often been exploited to modulate the process.

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Read about angiogenesis in the tumor microenvironment to gain insight into the cytokines involved.

Researching Angiogenesis

The molecular mechanisms that regulate angiogenesis have been the subject of intense study for the last 30+ years, particularly in the context of cancer. The cytokines, chemokines, growth factors, and proteases secreted by the various cancer cell types in the tumor microenvironment (TME) promote both angiogenesis and inflammation, two processes which appear to be interdependent and mutually reinforcing. RayBiotech provides you with a variety of tools to detect complex networks of soluble angiogenic and inflammatory factors and their receptors. With our wide selection of highly sensitive, rigorously tested antibody arrays and ELISAs, these proteins may be quantified individually or collectively from any body fluid, lysate, or cell culture supernatant.

What influences vascular growth and how can we modify it?

Use our arrays to simultaneously detection any or all of these angiogenesis targets

Activin A AgRP Angiopoietin-1 Angiopoietin-2 Angiogenin
Angiostatin ANGPTL4 bFGF CXCL16 EGF
ENA-78 (CXCL5) FGF-4 Follistatin GCSF GM-CSF
GRO alpha/beta/gamma HB-EGF HGF I-309 (TCA-3/CCL1) IFN-gamma
IGF-1 IL-10 IL-12 p40 IL-12 p70 IL-17A
IL-1 alpha (IL-1 F1) IL-1 beta (IL-1 F2) IL-2 IL-4 IL-6
IL-8 (CXCL8) IP-10 (CXCL10) I-TAC (CXCL11) Leptin LIF
MCP-1 (CCL2) MCP-2 (CCL8) MCP-3 (MARC/CCL7) MCP-4 (CCL13) MMP-1
MMP-9 PDGF-BB PECAM-1 (CD31) PLGF RANTES (CCL5)
TGF alpha TGF beta 1 TGF beta 3 Tie-1 Tie-2
TIMP-1 TIMP-2 TNF alpha TNF beta (TNFSF1B) Thrombopoietin (TPO)
uPAR VEGF-A VEGFR2 VEGFR3 VEGF-D



Array analysis of targets involved in angiogenesis are available in quantitative & semiquantitative formats.

Related Products

  1. Human Angiogenesis Array Q1
    Quantitative, sandwich-based, glass slide antibody array to detect 10 human angiogenic factors. Suitable for all liquid sample types.
  2. Human Angiogenesis Array Q2
    Quantitative, sandwich-based, glass slide antibody array to detect 30 human angiogenesis protein biomarkers.
  3. Human Angiogenesis Array C1
    Semi-quantitative, sandwich-based, membrane cytokine array to detect 20 human angiogenesis protein biomarkers.
  4. Human Angiogenesis Array Q1000
    Quantibody® Human Angiogenesis Array 1000 Kit. A combination of Human Angiogenesis Array Q2 & Q3. Detects 60 Human Angiogenic Factors. Suitable for all liquid sample types.
  5. Human VEGF-A ELISA

    RayBio® Human VEGF-A ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.

    Lead time: Typically ships within 1-2 business days. No Friday shipments.
  6. Human MCP-1 ELISA

    RayBio® Human MCP-1 (CCL2) ELISA Kit for cell culture supernatants, plasma, and serum samples.

    Lead time: Typically ships within 1-2 business days. No Friday shipments.

Featured Publications

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    Liu C., Wen J., Meng Y., et al. Efficient delivery of therapeutic miRNA nanocapsules for tumor suppression. Adv Mater. 2015 Jan 14;27(2):292-7. doi: 10.1002/adma.201403387
    Species: Human
    Sample type: Conditioned Media (U87 and MCF-7 cells transduced with micro RNAs)

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    Mause S., Ritzel E., Liehn E., et al. Platelet Microparticles Enhance the Vasoregenerative Potential of Angiogenic Early Outgrowth Cells After Vascular Injury. Circulation . 2010 Aug 3;122(5):495-506. doi: 10.1161/CIRCULATIONAHA.109.909473.
    Species: Human
    Sample type: Conditioned Media (HUVEC cells in vascular injury model)

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    Storti, P., et al. “HOXB7 expression by myeloma cells regulates their pro-angiogenic properties in multiple myeloma patients.” Leukemia 25.3 (2011): 527-537.
    Species: Human
    Sample type: Cell Lysate

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    Barcena C., Stefanociv M., Tutusaus A., et al. Angiogenin secretion from hepatoma cells activates hepatic stellate cells to amplify a self-sustained cycle promoting liver cancer. Sci Rep. 2015 Jan 21;5:7916. doi: 10.1038/srep07916.
    Species: Human
    Sample type: Conditioned Media (HepG2, Hep3B, and LX2 cells)

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    Greenberger S., Boscolo E., Adinit I., et al., Corticosteroid suppression of VEGF-A in infantile hemangioma-derived stem cells. N Engl J Med. 2010 Mar 18;362(11):1005-13. doi: 10.1056/NEJMoa0903036.
    Species: Human
    Sample type: Conditioned Media (Hemangioma derived Stem Cells)

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Related Research Areas

Tumor Microenvironment

Pro-inflammatory and pro-angiogenic factors can help to establish blood supply for the growing nascent tumor.

Phosphorylation Research Tools

Phosphorylation plays a critical role in virtually all cellular processes in homeostasis and disease, including signal transduction, cell cycle, differentiation, proliferation, metabolism, motility, and death.