Monkeypox Virus Research Products
Introduction
There is currently a multi-country outbreak of monkeypox in several regions of the world, the full extent and impact of which remains unclear. While usually endemic to the areas of central and west Africa, the virus has recently spread to over 48 countries and counting since being first identified in non-endemic regions. Currently the spread is believed to be minimal, the WHO, CDC, and other government entities are monitoring the situation closely.
Monkeypox virus is a virus from the orthopox family of viruses, a family most often associated with the more severe smallpox virus (variola), or the origination of vaccinations from the cowpox virus. Originally named for an outbreak among monkeys, it was first identified in humans in central and western Africa around 1970 where it continues to circulate at a low endemic level. While the virus is clinically less severe than smallpox, the symptoms are similar. With vaccinations against smallpox ceasing in 1972 and the eradication of the smallpox virus, vaccinations in the younger population (<50 years of age) is extremely limited.
The escape from the endemic regions has the World Health Organization on alert given the lack of associated travel within the region of those suspected and confirmed cases. Understanding how the virus is being spread, the populations it is spreading to, and the potential immune responses against the virus are of increasing importance in the research field.
1-Step High Throughput PCR Kit
Ready-to-use PCR assay for the detection of monkeypox virus (MPXV) DNA. The 1-Step Nucleic Acid Stabilization Buffer eliminates the need for DNA extraction and purification steps. You get the specificity of our Real-Time PCR kit combined with the easy-to-use 1 step extraction buffer.
Product performance index
- Limit of Detection: 4 copies / µL in the reaction
- Repeatability: Intra-assay %CV is ≤ 1.2%; Inter-assay %CV is ≤ 10%
- Specificity: No cross-reactivity with Cowpox virus, Camelpox virus, and Vaccinia virus
Real-Time PCR Kit
Ready-to-use PCR assay for the detection of Monkeypox virus (MPXV) DNA in a liquid sample. It uses a fluorescent probe with specific primer sets to detect the J2R gene specific for the genome of MPXV.
Product performance index
- Limit of Detection: 4 copies / µL in the reaction
- Repeatability: Intra-assay %CV is ≤ 1.2%; Inter-assay %CV is ≤ 10%
- Specificity: No cross-reactivity with Cowpox virus, Camelpox virus, and Vaccinia virus
Recombinant Proteins
RayBiotech has developed highly purified recombinant proteins for many unique Monkeypox virus proteins. These are in-stock and available for purchase online in 100 ug or 500 ug sizes.
Protein Specifications
- Sizes: 100 ug / 500 ug
- Format: Liquid
- Expression Systems: E. coli / HEK293
- Purification: His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC).
- Purity Determined By: SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
ELISA Kits
RayBiotech now offers antibody screening indirect ELISAs against 11 key MPXV antigens and a sandwich ELISA for MPXV A29. The indirect ELISA method is used to detect IgG, IgM, or IgA antibody isotypes. By quantifying the antibody within a sample, humoral immune response and exposure to MPXV can be assessed.
- Compatible with plasma and serum samples
- Pre-coated 96-well microplate
- High sensitivity
- Results within 3 hours
Protein Array
RayBiotech now provides glass slide-based protein arrays to screen IgG, IgM, IgA, or any other isotype antibody detection in serum, plasma, and other liquid sample types. Explore our already validated protein array or customize by browsing our catalog of proteins or by sending us your own. We can also make your desired proteins through our custom recombinant protein service.
- Measure IgG, IgM, or IgA antibody responses to immunization or patients
- Small sample volume required
- Custom kit includes buffers and detection reagents
- Ultra-sensitive, semi-quantitative fluorescence detection
- Partial or full testing services available
1-Step High Throughput PCR Kit Amplification Plot
Serially diluted Monkeypox Virus synthetic genomic DNA and fixed amount of Human RNase P DNA was spiked in VTM solution and mixed with equal volume of 1-Step Nucleic Acid Stabilization Buffer. Five microliter of the DNA-spiked sample that contains 104 , 103 , 102 , 10, 5, 2.5 and 1.25 copies/µl of Monkeypox Virus Synthetic Genomic DNA (MPXV, blue color) and fixed amount of RNase P template (RP, green color) were added to 15ul of master mix and amplified in a QuantStudio™ 5 Real-Time PCR.
