RayPlex™ Multiplex Bead Arrays

Take advantage of multiplex bead array technology for solution-based high throughput protein detection using a standard flow cytometer.

What Are RayPlex™ Multiplex Bead Arrays?

RayPlex™ Bead Arrays are a sandwich immunoassay that uses a capture antibody immobilized on the bead to detect a target protein, and a fluorescently-labeled detection antibody to visualize the captured target protein. By using different combinations of microbead sizes and fluorophores, you can simultaneously analyze multiple target proteins.

RayPlex™ is compatible with most standard flow cytometers that are equipped with blue and red lasers capable of detecting fluorescence in the phycoerythrin (PE) and allophycocyanin (APC) channels, respectively.

Armed with the precision that comes with sandwich immunoassay technology and the measurement capabilities of cytometric bead arrays, you can discover more deeply and more quickly with RayPlex™.

What will you discover next?

Need a customized array to meet your individual research objectives?

When your specific research questions aren’t addressed by a premade array, we’re here to help. With RayBiotech, you can access multiple array formats as well as the largest library of highly specific validated antibody pairs with over 800 target proteins.

Learn about our Custom Array Services>

How RayPlex™ Multiplex Bead Arrays Work

RayPlex how it works diagram

RayPlex™ fluorescent beads are pre-coated with capture antibodies.

  1. Incubate your sample with RayPlex™ beads. The proteins will bind to the corresponding capture antibody bead.
  2. Detect bound target by adding biotinylated detection antibodies.
  3. Wash away unbound protein and add PE-conjugated streptavidin.
  4. Analyze beads with a flow cytometer.
  5. Process and interpret the flow cytometry data.

Research Applications

We’ve seen the RayPlex Bead arrays used to address a large range of research questions. Here are a few of our favorite applications:

  • High-throughput profiling of cytokine expression.
  • Validation of semi-quantitative antibody array results.
  • Identifying potential molecular targets for drug development.
  • Identifying the molecular mechanisms of drug action.
  • Identifying crucial factors involved in disease processes.
  • Discovering biomarkers for disease management.
  • Discovering expression patterns for molecular classification of diseases.
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RayPlex Bead Arrays


  • Save sample without sacrificing data. You can quantify up to 25 proteins with only 25 µl of sample.
  • Get results faster with only 4 hours of processing time.
  • Save money with an array that’s 5-10x more cost-effective than ELISA.
  • Enjoy simple workflows with no specialized or dedicated equipment required (compatible with most flow cytometers).
  • Customize to fit your needs by creating a custom array from our list of targets.

Contents of Kit

  • RayPlex™ Multiplex Bead Cocktail
  • 1X Assay Diluent
  • 20X Wash Buffer
  • RayBio Serum Diluent
  • Lyophilized Protein Standard Mix
  • 10X Biotinylated Detection Antibody Cocktail
  • 100X Streptavidin PE
  • Rainbow Particles
  • Extra RayPlex™ Multiplex Bead Cocktail
  • V-bottom 96-well Microplate
  • Filter 96-well Microplate