Antibody Arrays

With a huge list of targets, formats, and kits to choose from and no special equipment requirements, you can build a comprehensive view of protein expression levels with a single antibody array study.

Discover More From Less Sample

Antibody arrays and antibody microarrays are multiplex assays that simultaneously measure the expression of multiple proteins, for more comprehensive insight than you can obtain with a single-target ELISA or western blot.

In addition, by measuring expression levels of multiple proteins from a single sample in a single study, you can obtain insight quickly and have more confidence in the comparability of your measurements than when you spend extra time, sample, and effort—and introduce additional variability—normalizing and comparing multiple experiments.

Have Complete Confidence In Your Results

Our arrays are shipped quickly, made in the USA, and produce reproducible results. We want you to succeed and provide support services and data analysis tools from our team of PhD scientists to help you get there. With the same antibody pairs as our ELISAs and validated in the appropriate array format, you know you’re getting reliable products that will set you up for success.

Antibody Array Categories

Cytokine Arrays

Simultaneously measure the levels of multiple cytokines in a range of biological fluids—available in membrane and glass slide formats.


Achieve quantitative, high-throughput protein expression analysis with an array-based multiplex ELISA system.

Membrane Antibody Arrays

Take advantage of the familiar chemiluminescent western blotting workflow while measuring relative expression of multiple proteins.

Label-based Antibody Arrays

Screen quickly and comprehensively with our largest array—available in membrane and glass slide formats.

Glass Slide Antibody Arrays

Obtain protein expression level data on multiple proteins while conserving precious sample—complementary laser scanning services available.

Popular Arrays

New Products

What customers are saying

“It is the only ELISA Array [Mouse Chemokine Array Q1, Cat# QAM-CHE-1] that gives a quantified assessment of 25 chemokines in one go. Protocol is easy to follow and Ray-biotech provides free scanning of slides. It is the best chemokine ELISA Array available.”


National Institutes of Health (US)

“The human apoptosis array [Human Apoptosis Signaling Array C1, Cat# AAH-APOSIG-1] provides a fast and comprehensive glance about the key signaling involved in the apoptotic pathway. It's a one shot experiment with certain controls already on the blot, pretty simple to execute and normalize. Would recommend it.”


Moffitt Cancer Research Center

microarray (1)

Featured Service

Array Testing Services

Whether you need extra bandwidth or additional expertise, you can advance confidently with comprehensive Array Testing Services from our experienced scientific team. Simply send us your samples and we’ll use our highly-regarded technology to generate the data you need. We can even handle the analysis through our Biostatistics and Bioinformatics services.

What is an Antibody Array?


Which Antibody Array Fits Your Application?

Applications Platform Design Principle Detection Data Type # of Analytes Species
Quantibody Protein Expression Profiling Slide* Sandwich-based, quadruplicate Fluorescent Quantitative 10-1000 All
C-Series Arrays Protein Expression Profiling Membrane Sandwich-based, duplicate Chemiluminescent Semi-Quantitative 10-274 H, M, R, B, C, D, E, G, O, P
G-Series Arrays Protein Expression Profiling Slide* Sandwich-based, duplicate or quadruplicate Fluorescent Semi-Quantitative 10-1000 All
L-Series Arrays Protein Expression Profiling Slide* or Membrane Label-based, duplicate Fluorescent or Chemiluminescent Semi-Quantitative 90-6000 H, M, R, L
RayPlex Arrays Protein Expression Profiling Microbead Sandwich-based Flow Cytometer Semi-Quantitative 14 H
Phospho Arrays Phosphorylation Profiling Slide* or Membrane Sandwich-based, quadruplicate Fluorescent or Chemiluminescent Semi-Quantitative 17-71 H, M
Lectin Arrays Protein-Lectin Interaction Slide* Label-based or Sandwich-based Fluorescent Semi-Quantitative 70-95 All
Glycome Arrays Glycosylation profiling Slide* Sandwich-based (lectin-ab pair) Fluorescent Semi-Quantitative 2000 All
Protein Arrays Auto-antibody profiling and characterizing antibody specificity Slide* Label-based Fluorescent Semi-Quantitative 22-48 H
Isotoping Arrays Immunoglobulin Expression Glass Chip Sandwich-based, duplicate Fluorescent Semi-Quantitative 10 M

H = human | M = mouse | R = rat | B = bovine | C = canine | F = feline | E = equine | P = porcine | L = rabbit | N = rhesus monkey | G = chicken | D = dolphin | O = ovine

*Our glass slide arrays require a laser scanner. If you don’t have access to a laser scanning instrument, we can scan them for you at no additional cost—learn more.

How to Choose an Antibody Array

Your Need Our Solution
I want to screen as many factors as possible (I need a "big net") L-Series: Label-based Arrays or larger Quantibody® Arrays
I want to focus on a specific pathway or biological process Pathway-specific arrays (e.g. Inflammation; Apoptosis, etc) or Phosphorylation Arrays
I want to choose a specific panel of markers Custom Array
I have a limited sample volume Glass Slide-based Arrays:
  • G-Series
  • L-Series (glass slide)
  • Quantibody® Arrays
I don't have a laser scanner Membrane-based Arrays:
  • C-Series
  • L-Series (membrane)
Or use our free glass slide scanning service
I want quantitative results Quantibody® Arrays
I want to identify antibody isotypes Isotyping Arrays
I want to study protein glycosylation Glycobiology Arrays (Lectin Arrays, Glycosylation Arrays, Glycan Arrays)
I want to screen protein-protein interactions Protein Arrays
I have samples from an uncommon species L-Series: Label-based Arrays or Quantibody® Arrays
I want to do biomarker discovery Any RayBiotech Array

How Antibody Arrays Can Advance Your Research


Frequently Asked Questions

Still have questions?

Quantitative data gives you the actual concentration (pg/ml, for example) of the analyte, while semi-quantitative data gives you the relative fold-change of the analyte compared to the concentration in another sample.

The main difference between the glass-slide and membrane-based arrays is the type of detection used. Glass-slide-based arrays use fluorescence and need a compatible laser scanner, while membrane-based arrays use chemiluminescence, which can be detected with most western blot imaging systems.

Another difference is the required sample size. Glass-slide assays use smaller sample sizes than membrane-based assays. Glass slides require ~70 ul – 100 ul, while the membranes require at least 1 ml, after dilution.

Lastly, glass-slide-based arrays are generally cheaper than membrane-based arrays.

For our membrane-based arrays, any chemiluminescence imaging system such as an X-ray film developer, CCD camera, or gel documentation system should work. Imaging systems using near-infrared flours, such as the Li-Cor Odyssey and Typhoon systems, also work extremely well with our membrane arrays.

For the glass slide-based arrays, you’ll need a gene microarray laser scanner. A list of specifications and compatible laser scanners can be found here:

Compatible Laser Scanners for Glass Slide Arrays

Most of our arrays are compatible with any biological fluid. This includes cell culture media, cell lysates, tissue lysates, and all clarified body fluids (serum, plasma, urine, cerebrospinal fluid, BAL, saliva, tears, etc.)

There are exceptions for label-based arrays (L-Series), which have limited compatibility with lysates and non-serum/plasma body fluids. Our phosphorylation arrays, which detect membrane-bound receptors, are only validated for use with cell and tissue lysates.

In general, any cell-free extract that contains soluble, non-denatured proteins is likely to work with our arrays.

Our tips for sample preparation can be found here:

Tips on Sample Preparation

The positive control spots are standardized amounts of biotinylated IgG. They are used for signal normalization, monitoring of the detection step, and to help orient the array image.

The negative control spots are printed with buffer only, and thus are not expected to give signals. Negative control spots are used for background subtraction.

The areas on the array map labeled as “blank” are empty (there is nothing printed there). Both the negative control and blank spots should give similar intensity values, and either one may be used to represent the background.

For our membrane arrays, data collection can be accomplished by any densitometry software. CCD camera-based imaging systems are typically equipped with a built-in densitometry software application.

Alternatively, there is a free densitometry program available through the NIH (Image J), which can be downloaded here. More information on how to use Image J can be found here.

For glass slide-based arrays, data extraction is accomplished by the software application associated with the laser scanner. For faster spot identification, we can provide you with a free GAL file which can be downloaded here. More information regarding glass slide array data extraction can be found here.

Normalization is used to compare data between arrays (i.e., different samples) by accounting for the differences in signal intensities of the positive control spots on those arrays. We can do this because the positive control is a controlled amount of biotinylated antibody that is attached to the solid support. The amount of signal from each of those spots is dependent on 1) the amount of the reporter (streptavidin-HRP or streptavidin-fluor) bound to that antibody and 2) the length of exposure time (for chemiluminescent detection).

Since these factors proportionally affect the signal intensity of every spot on the array, the differences in the positive control signals between arrays will accurately reflect the differences between other spots on those arrays.

One array must be defined as the "reference array“ to which the signals of other arrays are normalized. It is up to the researcher to define which array should be the reference.

You can calculate the normalized values as follows:
X(nY) = X(Y) x P1/P(Y)

  • P1 = the average signal density of the positive control spots on the reference array
  • P(Y) = the average signal density of the positive control spots on Array Y
  • X(Y) = the signal density for a particular spot on Array for sample “Y”
  • X(nY)= the normalized value for that particular spot "X" on Array for sample “Y"

Please note that RayBiotech’s analysis software tools can perform the normalization automatically. More information about our analysis tools can be found here.

No problem! Simply send your slides to us and we can scan them for you. We will provide you with the scanned image (TIF file) and the densitometry values with full analysis. Learn more about our scanning services here:

Scanning and Data Analysis Services

Different arrays have different sensitivities. You can find more information here:

Quantibody® Array

Human Cytokine Antibody Array

Mouse Cytokine Antibody Array

We’ve created a helpful guide to help you organize your slides. You can find it here:

Quantibody Standard Options

Still have questions?

Related Services

Related Products