Have Complete Confidence In Your Results
Our arrays are shipped quickly, made in the USA, and produce reproducible results. We want you to succeed and provide support services and data analysis tools from our team of PhD scientists to help you get there. With the same antibody pairs as our ELISAs and validated in the appropriate array format, you know you’re getting reliable products that will set you up for success.
Antibody Array Categories
Popular Arrays
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Wnt Pathway Screening Array Screen the relative levels of 123 human proteins involved in Wnt signaling pathway. Suitable for serum, plasma, cell culture supernatants, and lysates. -
mTOR Pathway Screening Array Screen the relative levels of 118 human proteins involved in mTOR signaling pathway. Suitable for serum, plasma, cell culture supernatants, and lysates.
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Toll/NOD-like Receptor Pathway Screening Array Screen the relative levels of 181 human proteins involved in Toll/NOD-like Receptor signaling pathway. Suitable for serum, plasma, cell culture supernatants, and lysates.
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Stem Cells Pluripotency Regulation Array Screen the relative levels of 119 human proteins involved in stem cells pluripotency regulation. Suitable for serum, plasma, cell culture supernatants, and lysates.
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Ras/Rap Pathway Screening Array Screen the relative levels of 253 human proteins involved in Ras/Rap signaling pathway. Suitable for serum, plasma, cell culture supernatants, and lysates.
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PPAR Pathway Screening Array Screen the relative levels of 54 human proteins involved in PPAR signaling pathway. Suitable for serum, plasma, cell culture supernatants, and lysates.
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Phosphorylation Screening Array Screen the phosphorylation and total levels of 500 human proteins. Suitable for serum, plasma, cell culture supernatants, and lysates.
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Oxytocin Pathway Screening Array Screen the relative levels of 108 human proteins involved in Oxytocin signaling pathway. Suitable for serum, plasma, cell culture supernatants, and lysates.
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NF-kappa B Pathway Screening Array Screen the relative levels of 90 human proteins involved in NF-kappa B signaling pathway. Suitable for serum, plasma, cell culture supernatants, and lysates.
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MAPK Pathway Screening Array Screen the relative levels of 237 human proteins involved in MAPK signaling pathway. Suitable for serum, plasma, cell culture supernatants, and lysates.
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Jak-STAT Pathway Screening Array Screen the relative levels of 152 human proteins involved in Jak-STAT signaling pathway. Suitable for serum, plasma, cell culture supernatants, and lysates.
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Hippo Pathway Screening Array Screen the relative levels of 118 human proteins involved in Hippo signaling pathway. Suitable for serum, plasma, cell culture supernatants, and lysates.
New Products
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Human Cytoskeleton Biomarker Screening Array Label-based antibody array kit using glass slide supports to screen and measure 153 human protein biomarkers involved with the cytoskeleton. Suitable for serum, plasma, lysates, and cell culture supernatants. -
Human Obesity Biomarker Screening Array Label-based antibody array kit using glass slide supports to screen and measure 307 human protein biomarkers involved in obesity. Suitable for serum, plasma, lysates, and cell culture supernatants.
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Human Neuro Disease Biomarker Screening Array Label-based antibody array kit using glass slide supports to screen and measure 500 human protein biomarkers involved in neurological diseases. Suitable for serum, plasma, lysates, and cell culture supernatants.
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Human Metabolic Pathway Biomarker Screening Array Label-based antibody array kit using glass slide supports to screen and measure 500 human protein biomarkers involved in the metabolic pathway. Suitable for serum, plasma, lysates, and cell culture supernatants.
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Human Metabolic Disease Biomarker Screening Array Label-based antibody array kit using glass slide supports to screen and measure 300 human protein biomarkers involved in metabolic diseases. Suitable for serum, plasma, lysates, and cell culture supernatants.
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Human Infectious Disease Biomarker Screening Array Label-based antibody array kit using glass slide supports to screen and measure 500 human protein biomarkers involved in infectious diseases. Suitable for serum, plasma, lysates, and cell culture supernatants.
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Human Endocrine Biomarker Screening Array Label-based antibody array kit using glass slide supports to screen and measure 500 human protein biomarkers involved in the endocrine system. Suitable for serum, plasma, lysates, and cell culture supernatants.
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Human DNA Damage Biomarker Screening Array Label-based antibody array kit using glass slide supports to screen and measure 308 human protein biomarkers involved in dna damage. Suitable for serum, plasma, lysates, and cell culture supernatants.
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Human Cardiovascular Disease Biomarker Screening Array Label-based antibody array kit using glass slide supports to screen and measure 284 human protein biomarkers involved in cardiovascular diseases. Suitable for serum, plasma, lysates, and cell culture supernatants.
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Human Cell Junction Biomarker Screening Array Label-based antibody array kit using glass slide supports to screen and measure 193 human protein biomarkers involved in cell junction. Suitable for serum, plasma, lysates, and cell culture supernatants.
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Human Cell Death Biomarker Screening Array Label-based antibody array kit using glass slide supports to screen and measure 263 human protein biomarkers involved in cell death. Suitable for serum, plasma, lysates, and cell culture supernatants.
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Human Cancer Biomarker Screening Array Label-based antibody array kit using glass slide supports to screen and measure 500 human protein biomarkers involved in cancer. Suitable for serum, plasma, lysates, and cell culture supernatants.
What customers are saying
“It is the only ELISA Array [Mouse Chemokine Array Q1, Cat# QAM-CHE-1] that gives a quantified assessment of 25 chemokines in one go. Protocol is easy to follow and Ray-biotech provides free scanning of slides. It is the best chemokine ELISA Array available.”
Shalini
National Institutes of Health (US)
“The human apoptosis array [Human Apoptosis Signaling Array C1, Cat# AAH-APOSIG-1] provides a fast and comprehensive glance about the key signaling involved in the apoptotic pathway. It's a one shot experiment with certain controls already on the blot, pretty simple to execute and normalize. Would recommend it.”
Vinayak
Moffitt Cancer Research Center
Featured Service
Array Testing Services
Whether you need extra bandwidth or additional expertise, you can advance confidently with comprehensive Array Testing Services from our experienced scientific team. Simply send us your samples and we’ll use our highly-regarded technology to generate the data you need. We can even handle the analysis through our Biostatistics and Bioinformatics services.
What is an Antibody Array?
Which Antibody Array Fits Your Application?
| Applications | Platform | Design Principle | Detection | Data Type | # of Analytes | Species | |
|---|---|---|---|---|---|---|---|
| Quantibody | Protein Expression Profiling | Slide* | Sandwich-based, quadruplicate | Fluorescent | Quantitative | 10-1000 | All |
| C-Series Arrays | Protein Expression Profiling | Membrane | Sandwich-based, duplicate | Chemiluminescent | Semi-Quantitative | 10-274 | H, M, R, B, C, D, E, G, O, P |
| G-Series Arrays | Protein Expression Profiling | Slide* | Sandwich-based, duplicate or quadruplicate | Fluorescent | Semi-Quantitative | 10-1000 | All |
| L-Series Arrays | Protein Expression Profiling | Slide* or Membrane | Label-based, duplicate | Fluorescent or Chemiluminescent | Semi-Quantitative | 90-6000 | H, M, R, L |
| RayPlex Arrays | Protein Expression Profiling | Microbead | Sandwich-based | Flow Cytometer | Semi-Quantitative | 14 | H |
| Phospho Arrays | Phosphorylation Profiling | Slide* or Membrane | Sandwich-based, quadruplicate | Fluorescent or Chemiluminescent | Semi-Quantitative | 17-71 | H, M |
| Lectin Arrays | Protein-Lectin Interaction | Slide* | Label-based or Sandwich-based | Fluorescent | Semi-Quantitative | 70-95 | All |
| Glycome Arrays | Glycosylation profiling | Slide* | Sandwich-based (lectin-ab pair) | Fluorescent | Semi-Quantitative | 2000 | All |
| Protein Arrays | Auto-antibody profiling and characterizing antibody specificity | Slide* | Label-based | Fluorescent | Semi-Quantitative | 22-48 | H |
| Isotoping Arrays | Immunoglobulin Expression | Glass Chip | Sandwich-based, duplicate | Fluorescent | Semi-Quantitative | 10 | M |
H = human | M = mouse | R = rat | B = bovine | C = canine | F = feline | E = equine | P = porcine | L = rabbit | N = rhesus monkey | G = chicken | D = dolphin | O = ovine
*Our glass slide arrays require a laser scanner. If you don’t have access to a laser scanning instrument, we can scan them for you at no additional cost—learn more.
How to Choose an Antibody Array
| Your Need | Our Solution |
|---|---|
| I want to screen as many factors as possible (I need a "big net") | L-Series: Label-based Arrays or larger Quantibody® Arrays |
| I want to focus on a specific pathway or biological process | Pathway-specific arrays (e.g. Inflammation; Apoptosis, etc) or Phosphorylation Arrays |
| I want to choose a specific panel of markers | Custom Array |
| I have a limited sample volume | Glass Slide-based Arrays:
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| I don't have a laser scanner | Membrane-based Arrays:
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| I want quantitative results | Quantibody® Arrays |
| I want to identify antibody isotypes | Isotyping Arrays |
| I want to study protein glycosylation | Glycobiology Arrays (Lectin Arrays, Glycosylation Arrays, Glycan Arrays) |
| I want to screen protein-protein interactions | Protein Arrays |
| I have samples from an uncommon species | L-Series: Label-based Arrays or Quantibody® Arrays |
| I want to do biomarker discovery | Any RayBiotech Array |
How Antibody Arrays Can Advance Your Research
Frequently Asked Questions
Still have questions?
Quantitative data gives you the actual concentration (pg/ml, for example) of the analyte, while semi-quantitative data gives you the relative fold-change of the analyte compared to the concentration in another sample.
The main difference between the glass-slide and membrane-based arrays is the type of detection used. Glass-slide-based arrays use fluorescence and need a compatible laser scanner, while membrane-based arrays use chemiluminescence, which can be detected with most western blot imaging systems.
Another difference is the required sample size. Glass-slide assays use smaller sample sizes than membrane-based assays. Glass slides require ~70 ul – 100 ul, while the membranes require at least 1 ml, after dilution.
Lastly, glass-slide-based arrays are generally cheaper than membrane-based arrays.
For our membrane-based arrays, any chemiluminescence imaging system such as an X-ray film developer, CCD camera, or gel documentation system should work. Imaging systems using near-infrared flours, such as the Li-Cor Odyssey and Typhoon systems, also work extremely well with our membrane arrays.
For the glass slide-based arrays, you’ll need a gene microarray laser scanner. A list of specifications and compatible laser scanners can be found here:
Most of our arrays are compatible with any biological fluid. This includes cell culture media, cell lysates, tissue lysates, and all clarified body fluids (serum, plasma, urine, cerebrospinal fluid, BAL, saliva, tears, etc.)
There are exceptions for label-based arrays (L-Series), which have limited compatibility with lysates and non-serum/plasma body fluids. Our phosphorylation arrays, which detect membrane-bound receptors, are only validated for use with cell and tissue lysates.
In general, any cell-free extract that contains soluble, non-denatured proteins is likely to work with our arrays.
Our tips for sample preparation can be found here:
The positive control spots are standardized amounts of biotinylated IgG. They are used for signal normalization, monitoring of the detection step, and to help orient the array image.
The negative control spots are printed with buffer only, and thus are not expected to give signals. Negative control spots are used for background subtraction.
The areas on the array map labeled as “blank” are empty (there is nothing printed there). Both the negative control and blank spots should give similar intensity values, and either one may be used to represent the background.
For our membrane arrays, data collection can be accomplished by any densitometry software. CCD camera-based imaging systems are typically equipped with a built-in densitometry software application.
Alternatively, there is a free densitometry program available through the NIH (Image J), which can be downloaded here. More information on how to use Image J can be found here.
For glass slide-based arrays, data extraction is accomplished by the software application associated with the laser scanner. For faster spot identification, we can provide you with a free GAL file which can be downloaded here. More information regarding glass slide array data extraction can be found here.
Normalization is used to compare data between arrays (i.e., different samples) by accounting for the differences in signal intensities of the positive control spots on those arrays. We can do this because the positive control is a controlled amount of biotinylated antibody that is attached to the solid support. The amount of signal from each of those spots is dependent on 1) the amount of the reporter (streptavidin-HRP or streptavidin-fluor) bound to that antibody and 2) the length of exposure time (for chemiluminescent detection).
Since these factors proportionally affect the signal intensity of every spot on the array, the differences in the positive control signals between arrays will accurately reflect the differences between other spots on those arrays.
One array must be defined as the "reference array“ to which the signals of other arrays are normalized. It is up to the researcher to define which array should be the reference.
You can calculate the normalized values as follows:
X(nY) = X(Y) x P1/P(Y)
- P1 = the average signal density of the positive control spots on the reference array
- P(Y) = the average signal density of the positive control spots on Array Y
- X(Y) = the signal density for a particular spot on Array for sample “Y”
- X(nY)= the normalized value for that particular spot "X" on Array for sample “Y"
Please note that RayBiotech’s analysis software tools can perform the normalization automatically. More information about our analysis tools can be found here.
No problem! Simply send your slides to us and we can scan them for you. We will provide you with the scanned image (TIF file) and the densitometry values with full analysis. Learn more about our scanning services here:
Different arrays have different sensitivities. You can find more information here:
We’ve created a helpful guide to help you organize your slides. You can find it here:
Still have questions?
