Bovine Cytokine Array C1

RayBio® C-Series Bovine Cytokine Antibody Array 1 Kit. Detects 30 Bovine Cytokines. Suitable for all liquid sample types.

$416.12  
AAB-CYT-1-2
+

Antigen Information

Gene Symbols:
ANGPT1 | C1QTNF1 | CCL2 | CD40LG | CXCL10 | CXCL9 | DCN | FGF1 | FGF2 | IFNG   View more

Assay Format

Number of Targets Detected:
30
Species Detected:
  • Bovine (cow)
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
aFGF
(FGF-1)
Angiopoietin-1
bFGF
CD40 Ligand
(TNFSF5)
  • Gene Symbol: CD40LG CD40L TNFSF5
  • Gene ID: 282387
  • Accession #: P51749
Decorin
GASP-1
(WFIKKNRP)
IFN alpha
IFN-beta
IFN-gamma
IGF-1
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
  • Gene Symbol: IL1B
  • Gene ID: 281251
  • Accession #: P09428
IL-36 Ra
(IL-1 F5)
IL-10
  • Gene Symbol: IL10
  • Gene ID: 281246
  • Accession #: P43480
IL-13
IL-15
IL-17A
IL-18
  • Gene Symbol: IL18 IGIF
  • Gene ID: 281249
  • Accession #: Q9TU73
IL-2
IL-21
IL-4
IP-10
(CXCL10)
LIF
  • Gene Symbol: LIF
  • Gene ID: 280840
  • Accession #: Q27956
MCP-1
(CCL2)
MIG
(CXCL9)
MIP-1 beta
(CCL4)
NCAM-1
(CD56)
RANTES
(CCL5)
TNF alpha
VEGF-A

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Bovine Cytokine Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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