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Assay Format

Number of Targets Detected:

Species Detected:

Canine (dog)

Compatible Sample Types:

Cell Culture Supernatants
Cell Lysates
Plasma
Serum
Tissue Lysates

Design Principle:

Sandwich-based

Method of Detection:

Chemiluminescence

Quantitative/Semi-Quantitative:

Semi-Quantitative

Solid Support:

Membrane

Product Specifications

Size:

2, 4, or 8 Sample Kit

Protein Information

Gene Symbols:

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Cystatin C ELISA Kit: RayBio Canine Cystatin C ELISA	Decorin Gene Symbol: DCN DCN1C Gene ID: 403904 Accession #: Q29393 ELISA Kit: RayBio Canine Decorin ELISA	EGFR Gene Symbol: EGFR Accession #: Q6QT04 ELISA Kit: RayBio Canine EGFR ELISA	ErbB3 ELISA Kit: RayBio Canine ErbB3 ELISA	Erythropoietin Gene Symbol: EPOR Gene ID: 484943 Accession #: Q2KL21
FGF-7 (KGF) Gene Symbol: FGF7 EGK_17479 Gene ID: 574345 Accession #: F7A1B0	Galectin-3 Gene Symbol: LGALS3 Gene ID: Accession #: P38486 ELISA Kit: RayBio Canine Galectin-3 ELISA	GASP-1 (WFIKKNRP) ELISA Kit: RayBio Canine GASP-1 ELISA	GM-CSF Gene Symbol: CSF2 Gene ID: 403923 Accession #: P48749 ELISA Kit: RayBio Canine GM-CSF ELISA	GRO alpha (CXCL1) Gene Symbol: Cxcl1 ELISA Kit: RayBio Canine CXCL1 ELISA
HGF Gene Symbol: HGF Gene ID: 403441 Accession #: Q867B7 ELISA Kit: RayBio Canine HGF ELISA	HGFR Gene Symbol: MET Gene ID: 403438 Accession #: Q75ZY9 ELISA Kit: RayBio Canine HGF Receptor ELISA	IFN-gamma Gene Symbol: IFNG Gene ID: 403801 Accession #: P42161 ELISA Kit: RayBio Canine IFN-gamma ELISA	IGFBP-2 ELISA Kit: RayBio Canine IGFBP-2 ELISA	IL-1 alpha (IL-1 F1)
IL-1 beta (IL-1 F2) Gene Symbol: IL1B Gene ID: Accession #: Q28292 ELISA Kit: RayBio Canine IL-1 beta ELISA	IL-1 Ra (IL-1 F3)	IL-10 Gene Symbol: IL10 Gene ID: 403628 Accession #: P48411 ELISA Kit: RayBio Canine IL-10 ELISA	IL-12 p40 Gene Symbol: IL12B Gene ID: 403976 Accession #: Q28268 ELISA Kit: RayBio Canine IL-12 p40 ELISA	IL-13
IL-17A Gene Symbol: IL-17A IL17A Gene ID: 481837 Accession #: C6L8D7	IL-2 Gene Symbol: IL2 Gene ID: 403989 Accession #: Q29416 ELISA Kit: RayBio Canine IL-2 ELISA	IL-21	IL-6 Gene Symbol: IL6 Gene ID: 403985 Accession #: P41323 ELISA Kit: RayBio Canine IL-6 ELISA	IL-8 (CXCL8) Gene Symbol: CXCL8 IL8 Gene ID: 403850 Accession #: P41324 ELISA Kit: RayBio Canine IL-8 ELISA
MCP-1 (CCL2) Gene Symbol: CCL2 MCP1 SCYA2 Gene ID: 403981 Accession #: P52203 ELISA Kit: RayBio Canine MCP-1 ELISA	MIP-1 beta (CCL4) Gene Symbol: CCL4 SCYA4 Gene ID: 448786 Accession #: Q68AZ0	MMP-8 ELISA Kit: RayBio Canine MMP-8 ELISA	NCAM-1 (CD56) ELISA Kit: RayBio Canine NCAM-1 ELISA	Nope ELISA Kit: RayBio Canine Nope ELISA
PDGF-BB Gene Symbol: PDGFB Gene ID: 442986 Accession #: Q6Q7I7 ELISA Kit: RayBio Canine PDGF-BB ELISA	RAGE Gene ID: 403168 Accession #: Q24LN3 ELISA Kit: RayBio Canine RAGE ELISA	RANTES (CCL5)	Resistin ELISA Kit: RayBio Canine Resistin ELISA	SCF Gene Symbol: KITLG MGF Gene ID: 403507 Accession #: Q06220 ELISA Kit: RayBio Canine SCF Ligand ELISA
TGF alpha ELISA Kit: RayBio Canine TGF alpha ELISA	TNF alpha Gene Symbol: TNF TNFA TNFSF2 Gene ID: 403922 Accession #: P51742 ELISA Kit: RayBio Canine TNF-alpha ELISA	TNF RI (TNFRSF1A) Gene Symbol: TNFRSF1A Gene ID: 403634; Accession #: F1PNY7	Trappin-2 ELISA Kit: RayBio Canine Trappin-2 ELISA	VEGF-A Gene Symbol: VEGFA VEGF Gene ID: 403802 Accession #: Q9MYV3 ELISA Kit: RayBio Canine VEGF-A ELISA

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Canine Cytokine Antibody Array C1 Membranes
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated HRP
Detection Buffer C
Detection Buffer D
Lysis Buffer
8-Well Incubation Tray
Plastic Sheets
Array Templates
Manual

Other Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It^® or EpiChem II Benchtop Darkroom or GE's ImageQuant^™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20^°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20^°C and all other reagents undiluted at 4^°C for no more than 3 months.

Very easy to use, sensitive to the cytokines. Will definitely use again!

Garrett Grocke
University of Chicago 10/27/2019, 10:04 PM

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