Human Angiogenesis Array C1

RayBio® C-Series Human Angiogenesis Antibody Array 1 Kit. Detects 20 Human Angiogenic Factors. Suitable for all liquid sample types.

$311.08  
AAH-ANG-1-2
+

Antigen Information

Gene Symbols:
ANG | CCL2 | CCL5 | CXCL1 | CXCL2 | CXCL3 | CXCL5 | CXCL8 | EGF | FGF2   View more

Assay Format

Number of Targets Detected:
20
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
4 or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Angiogenin
EGF
ENA-78
(CXCL5)
bFGF
GRO alpha/beta/gamma
IFN-gamma
IGF-1
IL-6
IL-8
(CXCL8)
Leptin
MCP-1
(CCL2)
PDGF-BB
PLGF
RANTES
(CCL5)
TGF beta 1
TIMP-1
TIMP-2
Thrombopoietin
(TPO)
VEGF-A
VEGF-D

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process; Detecting Nephrotoxicity
Kit Components
  • Human Angiogenesis Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Picardi P., et al. Anandamide inhibits breast tumor-induced angiogenesis. . Transl Med UniSa. 2014 Sep-Dec; 10: 8-12.
    Species: Human
    Sample type: Conditioned Media
  2. Santo S., Seiler S., Fuchs A. ,Staudigl J., Widmer H. The Secretome of Endothelial Progenitor Cells Promotes Brain Endothelial Cell Activity through PI3-Kinase and MAP-Kinase. PLOSOne, Published: April 22, 2014, DOI: 10.1371/journal.pone.0095731 [Epub ahead of Print]
    Species: Human
    Sample type: Conditioned Media
  3. Zaytseva Y., et al. Cancer cell-associated fatty acid synthase activates endothelial cells and promotesangiogenesis in colorectal cancer. Carcinogenesis vol.35 no.6 pp.1341-1351, 2014. doi:10.1093/carcin/bgu042
    Species: Human
    Sample type: Conditioned Media
  4. De Boeck A., et al. Differential secretome analysis of cancer-associated fibroblasts and bone marrow-derived precursors to identify microenvironmental regulators of colon cancer progression. Proteomics. 2013 Jan;13(2):379-88. doi: 10.1002/pmic.201200179. Epub 2013 Jan 6.
    Species: Human
    Sample type: Conditioned Media
  5. Zhang, Xiaotun, et al. "Protease-activated receptor 2 signaling upregulates angiogenic growth factors in renal cell carcinoma." Experimental and molecular pathology 94.1 (2013): 91-97.
    Species: Human
    Sample type: Conditioned Media
  6. Kobayashi, Mitsuyoshi, et al. "Antitumor effect of metformin in esophageal cancer: in vitro study." International journal of oncology 42.2 (2013): 517-524.
    Species: Human
    Sample type: Cell Lysate
  7. Schaafhausen, Maximilian K., et al. "Tumor angiogenesis is caused by single melanoma cells in a manner dependent on reactive oxygen species and NF-?B." J Cell Sci 126.17 (2013): 3862-3872.
    Species: Human
    Sample type: Conditioned Media
  8. Maussang, David, et al. "Llama-derived single variable domains (nanobodies) directed against chemokine receptor CXCR7 reduce head and neck cancer cell growth in vivo." Journal of Biological Chemistry 288.41 (2013): 29562-29572.
    Species: Human
    Sample type: Conditioned Media
  9. Chang, Shu-Chun, and Wei-Chung Vivian Yang. "Hyperglycemia induces altered expressions of angiogenesis associated molecules in the trophoblast." Evidence-Based Complementary and Alternative Medicine 2013 (2013).
    Species: Human
    Sample type: Cell Lysate
  10. Dwyer J., Hebda JK., Le Guelte A., et al. Glioblastoma cell-secreted interleukin-8 induces brain endothelial cell permeability via CXCR2. PLoS One. 2012;7(9):e45562. doi: 10.1371/journal.pone.0045562
    Species: Human
    Sample type: Conditioned Media
I used this kit to detect the angiogenesis related cytokines in plasma and it works fine.

Purdue University
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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