Human Angiogenesis Array C1

RayBio® C-Series Human Angiogenesis Antibody Array 1 Kit. Detects 20 Human Angiogenic Factors. Suitable for all liquid sample types.

$311.08  
AAH-ANG-1-2
+

Antigen Information

Gene Symbols:
ANG | CCL2 | CCL5 | CXCL1 | CXCL2 | CXCL3 | CXCL5 | CXCL8 | EGF | FGF2   View more

Assay Format

Number of Targets Detected:
20
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
4 or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Angiogenin
EGF
ENA-78
(CXCL5)
bFGF
GRO alpha/beta/gamma
IFN-gamma
IGF-1
IL-6
IL-8
(CXCL8)
Leptin
MCP-1
(CCL2)
PDGF-BB
PLGF
RANTES
(CCL5)
TGF beta 1
TIMP-1
TIMP-2
Thrombopoietin
(TPO)
VEGF-A
VEGF-D

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process; Detecting Nephrotoxicity
Kit Components
  • Human Angiogenesis Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Hossain A., Gumin J., Gao F., et al. Mesenchymal Stem Cells Isolated From Human Gliomas Increase Proliferation and Maintain Stemness of Glioma Stem Cells Through the IL-6/gp130/STAT3 Pathway. Stem Cells. 2015 Aug;33(8):2400-15. doi: 10.1002/stem.2053.
    Species: Human
    Sample type: Conditioned Media
  2. Ulasov I., Borovjagin A., Kaverina N., et al. MT1-MMP silencing by an shRNA-armed glioma-targeted conditionally replicative adenovirus (CRAd) improves its anti-glioma efficacy in vitro and in vivo. Cancer Lett. 2015 Sep 1;365(2):240-50. doi: 10.1016/j.canlet.2015.06.002.
    Species: Human
    Sample type: Conditioned Media
  3. Miyata M., Morishita A., Sakamoto T., et al. MicroRNA profiles in cisplatin-induced apoptosis of hepatocellular carcinoma cells. International Journal of Oncology, August 2015 Volume 47 Issue 2 Pages: 535-542 DOI: 10.3892/ijo.2015.3036
    Species: Human
    Sample type: Conditioned Media
  4. Joo H., Song S., Seo H., et al. Human endothelial colony forming cells from adult peripheral blood have enhanced sprouting angiogenic potential through up-regulating VEGFR2 signaling. Int J Cardiol. 2015 Oct 15;197:33-43. doi: 10.1016/j.ijcard.2015.06.013.
    Species: Human
    Sample type: Cell Lysate
  5. Bodnar R., Wells A. Differential regulation of pericyte function by the CXC receptor 3. Wound Repair Regen. 2015 Nov-Dec;23(6):785-96. doi: 10.1111/wrr.12346.
    Species: Human
    Sample type: Conditioned Media
  6. Kobayashi K., Morishita A., Iwama H., et al. Galectin-9 suppresses cholangiocarcinoma cell proliferation by inducing apoptosis but not cell cycle arrest. Oncol Rep. 2015 Oct;34(4):1761-70. doi: 10.3892/or.2015.4197
    Species: Human
    Sample type: Conditioned Media
  7. Unterman S., Freiman A., Beckerman M., et al. Tuning of collagen scaffold properties modulates embedded endothelial cell regulatory phenotype in repair of vascular injuries in vivo. Adv Healthc Mater. 2015 Oct 28;4(15):2220-8. doi: 10.1002/adhm.201500457.
    Species: Human
    Sample type: Conditioned Media
  8. Al-Shammari A. Allak W., Umran M., et al. Angiogenesis Factors Associated with New Breast Cancer Cell Line AMJ13 Cultured in Vitro. Advances in Breast Cancer Research, 2015, 4, 100-108, http://dx.doi.org/10.4236/abcr.2015.44011
    Species: Human
    Sample type: Conditioned Media
  9. Ash S., et al. Xenon decreases cell migration and secretion of a pro-angiogenesis factor in breast adenocarcinoma cells: comparison with sevoflurane. Br. J. Anaesth. (2014) doi: 10.1093/bja/aeu191
    Species: Human
    Sample type: Conditioned Media
  10. O'Toole A., et al. Tumour microenvironment of both early- and late-stage colorectal cancer is equally immunosuppressive. British Journal of Cancer (2014), 1-6 | doi: 10.1038/bjc.2014.367
    Species: Human
    Sample type: Conditioned Media
I used this kit to detect the angiogenesis related cytokines in plasma and it works fine.

Purdue University
Write A Review

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

C-Series Testing Service C-Series Testing Service

Amazon Gift Card Program

Related Products Related Products

Customers Also Bought Customers Also Bought