Human Angiogenesis Array C2

RayBio® C-Series Human Angiogenesis Antibody Array 2 Kit. Detects 23 Human Angiogenic Factors. Suitable for all liquid sample types.

AAH-ANG-2-2
+

Antigen Information

Gene Symbols:
ANGPT1 | ANGPT2 | CCL1 | CCL13 | CCL7 | COL18A1 | CSF2 | CSF3 | CXCL11 | FLT4   View more

Assay Format

Number of Targets Detected:
23
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
4 or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Angiopoietin-1
Angiopoietin-2
Angiostatin
Endostatin
GCSF
GM-CSF
I-309
(TCA-3/CCL1)
IL-10
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-2
IL-4
I-TAC
(CXCL11)
MCP-3
(MARC/CCL7)
MCP-4
(CCL13)
MMP-1
MMP-9
PECAM-1
(CD31)
Tie-2
TNF alpha
uPAR
VEGFR2
VEGFR3

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Angiogenesis Antibody Array C2 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Jin YP., Hu YP., Wu XS., et al. miR-143-3p targeting of ITGA6 suppresses tumour growth and angiogenesis by downregulating PLGF expression via the PI3K/AKT pathway in gallbladder carcinoma. Cell Death Dis. 2018 Feb; 9(2): 182. 2018 Feb 7. doi: 10.1038/s41419-017-0258-2
    Species: Human
    Sample type: Conditioned Media
  2. Rezzola S., Corsini M., Chiodelli P., et al. Inflammation and N-formyl peptide receptors mediate the angiogenic activity of human vitreous humour in proliferative diabetic retinopathy. Diabetologia. 2017 Apr;60(4):719-728. doi: 10.1007/s00125-016-4204-0.
    Species: Human
    Sample type: Vitreous Fluid
  3. Zhu L., Cheng X., Shi J., et al. Crosstalk between bone marrow-derived myofibroblasts and gastric cancer cells regulates cancer stemness and promotes tumorigenesis. Oncogene advance online publication 25 April 2016; doi: 10.1038/onc.2016.76
    Species: Mouse
    Sample type: Conditioned Media
  4. Li C., Zhang Y., Tang L., et al. Expression of factors involved in the regulation of angiogenesis in the full-term human placenta: Effects of in vitro fertilization. Reprod Biol. 2016 Jun;16(2):104-12. doi: 10.1016/j.repbio.2016.02.003.
    Species: Human
    Sample type: Tissue lysate
  5. Chung E., Rytlewski J., Merchant AG., et al. Fibrin-based 3D matrices induce angiogenic behavior of adipose-derived stem cells. Acta Biomaterialia Available online 16 January 2015. doi:10.1016/j.actbio.2015.01.012
    Species: Human
    Sample type: Conditioned Media
  6. Shimura T., Dagher A., Sachdev M., et al. Urinary ADAM12 and MMP-9/NGAL Complex Detect the Presence of Gastric Cancer. Cancer Prevention Research, Published Online First January 15, 2015; doi: 10.1158/1940-6207.CAPR-14-0229
    Species: Human
    Sample type: Urine
  7. Hossain A., Gumin J., Gao F., et al. Mesenchymal Stem Cells Isolated From Human Gliomas Increase Proliferation and Maintain Stemness of Glioma Stem Cells Through the IL-6/gp130/STAT3 Pathway. Stem Cells. 2015 Aug;33(8):2400-15. doi: 10.1002/stem.2053.
    Species: Human
    Sample type: Conditioned Media
  8. Joo H., Song S., Seo H., et al. Human endothelial colony forming cells from adult peripheral blood have enhanced sprouting angiogenic potential through up-regulating VEGFR2 signaling. Int J Cardiol. 2015 Oct 15;197:33-43. doi: 10.1016/j.ijcard.2015.06.013.
    Species: Human
    Sample type: Cell Lysate
  9. Bodnar R., Wells A. Differential regulation of pericyte function by the CXC receptor 3. Wound Repair Regen. 2015 Nov-Dec;23(6):785-96. doi: 10.1111/wrr.12346.
    Species: Human
    Sample type: Conditioned Media
  10. Wu H., Fan F., Liu Z., et al. The angiogenic responses induced by release of angiogenic proteins from tumor cell-activated platelets are regulated by distinct molecular pathways. IUBMB Life. 2015 Aug;67(8):626-33. doi: 10.1002/iub.1406
    Species: Human
    Sample type: Platelet Releasate

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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