Human Angiogenesis Array C1000

RayBio® C-Series Human Angiogenesis Antibody Array 1000 Kit. A combination of Human Angiogenesis Antibody Array C1 & C2. Detects 43 Human Angiogenic Factors. Suitable for all liquid sample types.

$465.61  
AAH-ANG-1000-2
+

Antigen Information

Gene Symbols:
ANG | ANGPT1 | ANGPT2 | CCL1 | CCL13 | CCL2 | CCL5 | CCL7 | COL18A1 | CSF2   View more

Assay Format

Number of Targets Detected:
43
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
4 or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Angiogenin
Angiopoietin-1
Angiopoietin-2
Angiostatin
bFGF
EGF
ENA-78
(CXCL5)
Endostatin
GCSF
GM-CSF
GRO alpha/beta/gamma
I-309
(TCA-3/CCL1)
IFN-gamma
IGF-1
IL-10
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-2
IL-4
IL-6
IL-8
(CXCL8)
I-TAC
(CXCL11)
Leptin
MCP-1
(CCL2)
MCP-3
(MARC/CCL7)
MCP-4
(CCL13)
MMP-1
MMP-9
PDGF-BB
PECAM-1
(CD31)
PLGF
RANTES
(CCL5)
TGF beta 1
Thrombopoietin
(TPO)
Tie-2
TIMP-1
TIMP-2
TNF alpha
uPAR
VEGF-A
VEGFR2
VEGFR3
VEGF-D

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Angiogenesis Antibody Array C1000 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Choi Y., Cha Y., Lee K., et al. Proangiogenic cells enhanced persistent and physiologic neovascularization compared with macrophages. Exp Mol Med. 2015 Sep 25;47:e186. doi: 10.1038/emm.2015.60.
    Species: Human
    Sample type: Conditioned Media
  2. Konig J., et al. Placental mesenchymal stromal cells derived from blood vessels or avascular tissues: What is the better choice to support endothelial cell function?. Stem Cells and Development, Sept 2014 epub ahead of print. doi:10.1089/scd.2014.0115)
    Species: Human
    Sample type: Conditioned Media
  3. Perotti, Cesare, et al. "Allogeneic lethally irradiated cord blood mononuclear cells in no-option critical limb ischemia: a “box of rain”." Stem cells and development 22.20 (2013): 2806-2812.
    Species: Human
    Sample type: Conditioned Media
  4. Lung H., Cheung A., Ko J., et al Identification of Tumor Suppressor Genes via Cell Fusion and Chromosomal Transfer. Tumor Suppressor Genes, book edited by Yue Cheng, ISBN 978-953-307-879-3, Published: February 3, 2012 DOI: 10.5772/38925
    Species: Human
    Sample type: Conditioned Media
  5. Abajo A., Bitarte N., Zarate R., et al. Identification of colorectal cancer metastasis markers by an angiogenesis-related cytokine-antibody array. World J Gastroenterol. 2012 Feb 21;18(7):637-45. doi: 10.3748/wjg.v18.i7.637.
    Species: Human
    Sample type: Conditioned Media
  6. Li K., Huang Y., Ho C., et al. The role of IL-8 in the SDF-1?/CXCR4-induced angiogenesis of laryngeal and hypopharyngeal squamous cell carcinoma. Oral Oncol. 2012 Jun;48(6):507-15. doi: 10.1016/j.oraloncology.2012.01.006.
    Species: Human
    Sample type: Cell Lysate
  7. Fontanilla M., Espinosa L. In vitro and in vivo assessment of oral autologous artificial connective tissue characteristics that influence its performance as a graft. Tissue Eng Part A. 2012 Sep;18(17-18):1857-66. doi: 10.1089/ten.TEA.2011.0421.
    Species: Human
    Sample type: Conditioned Media
  8. Moubarik, Chahrazad, et al. "Transplanted late outgrowth endothelial progenitor cells as cell therapy product for stroke." Stem Cell Reviews and Reports 7.1 (2011): 208-220.
    Species: Human
    Sample type: Conditioned Media
  9. Kim, Hwi Gon, and Ook Hwan Choi. "Neovascularization in a mouse model via stem cells derived from human fetal amniotic membranes." Heart and vessels 26.2 (2011): 196-205.
    Species: Human
    Sample type: Conditioned Media
  10. Kim, Eun Ju, et al. "UV modulation of subcutaneous fat metabolism." Journal of Investigative Dermatology 131.8 (2011): 1720-1726.
    Species: Human
    Sample type: Conditioned Media

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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