Human Apoptosis Array C1

RayBio® C-Series Human Apoptosis Antibody Array 1 Kit. Detects 43 Human Apoptotic Factors. Suitable for all liquid sample types but intended for use with cell and tissue lysates.

AAH-APO-1-2
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Antigen Information

Gene Symbols:
BAD | BAX | BCL2 | BCL2L11 | BCL2L2 | BID | BIRC3 | BIRC5 | BIRC7 | CASP3   View more

Assay Format

Pathway:
  • Akt Signaling
  • IGF Signaling
  • p53 Signaling
Number of Targets Detected:
43
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
bad
bax
  • Gene Symbol: BAX
  • Gene ID: 581
  • Accession #: Q07812
bcl-2
  • Gene Symbol: BCL2
  • Gene ID: 596
  • Accession #: P10415
bcl-w
  • Gene Symbol: BCL2L2
  • Gene ID: 599
  • Accession #: Q92843
BID
BIM
  • Gene Symbol: BCL2L11
  • Gene ID: 10018
  • Accession #: Q43521
Caspase-3
  • Gene Symbol: CASP3
  • Gene ID: 836
  • Accession #: P42574
Caspase-8
  • Gene Symbol: CASP8
  • Gene ID: 841
  • Accession #: Q14790
CD40
(TNFRSF5)
CD40 Ligand
(TNFSF5)
cIAP-2
Cytochrome C
DR6
(TNFRSF21)
Fas
(TNFRSF6/Apo-1)
Fas Ligand
(TNFSF6)
HSP27
HSP60
  • Gene Symbol: HSPA14
  • Gene ID: 51182
  • Accession #: Q0VDF9
HSP70
HTRA2
IGF-1
IGF-2
  • Gene Symbol: Igf2
  • Gene ID: 3481
  • Accession #: P01344
IGFBP-1
IGFBP-2
IGFBP-3
IGFBP-4
IGFBP-5
IGFBP-6
IGF-1 R
livin
p21
  • Gene Symbol: CDKN1A
  • Gene ID: 1026
  • Accession #: P38936
p27
p53
SMAC
Survivin
(BIRC5)
  • Gene Symbol: BIRC5
  • Gene ID: 332
  • Accession #: O15392
TNF RI
(TNFRSF1A)
TNF RII
(TNFRSF1B)
TNF alpha
TNF beta
(TNFSF1B)
TRAIL R1
(TNFRSF10A/DR4)
TRAIL R2
(TNFRSF10B/DR5)
TRAIL R3
(TNFRSF10C)
TRAIL R4
(TNFRSF10D)
XIAP

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Apoptosis Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • Protease Inhibitor Cocktail
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Li L., Rola A., Kaid F., et al. Goniothalamin induces cell cycle arrest and apoptosis in H400 human oral squamous cell carcinoma: A caspase-dependent mitochondrial-mediated pathway with downregulation of NF-??. Arch Oral Biol. 2016 Apr;64:28-38. doi: 10.1016/j.archoralbio.2015.12.002.
    Species: Human
    Sample type: Cell Lysate
  2. Jackson R., Tilokee E., Latham N., et al. Paracrine Engineering of Human Cardiac Stem Cells With Insulin-Like Growth Factor 1 Enhances Myocardial Repair. J Am Heart Assoc. 2015 Sep 11;4(9):e002104. doi: 10.1161/JAHA.115.002104.
    Species: Human
    Sample type: Cell Lysate
  3. Lee C., Joo Y., Baek H., et al. Different effects of five depigmentary compounds, rhododendrol, raspberry ketone, monobenzone, rucinol and AP736 on melanogenesis and viability of human epidermal melanocytes. Exp Dermatol. 2016 Jan;25(1):44-9. doi: 10.1111/exd.12871.
    Species: Human
    Sample type: Cell Lysate
  4. Nordin N., Fadaeinasab M., Mohan S., et al. Pulchrin A, a New Natural Coumarin Derivative of Enicosanthellum pulchrum, Induces Apoptosis in Ovarian Cancer Cells via Intrinsic Pathway. PLoS One. 2016 May 2;11(5):e0154023. doi: 10.1371/journal.pone.0154023.
    Species: Human
    Sample type: Cell Lysate
  5. Almosnid N., Gao Y., He C., et al. In vitro antitumor effects of two novel oligostilbenes, cis- and trans-suffruticosol D, isolated from Paeonia suffruticosa seeds. Int J Oncol. 2016 Feb;48(2):646-56. doi: 10.3892/ijo.2015.3269.
    Species: Human
    Sample type: Cell Lysate
  6. Ahmadipour F., Noordin M., Mohan S., et al. Koenimbin, a natural dietary compound of Murraya koenigii (L) Spreng: inhibition of MCF7 breast cancer cells and targeting of derived MCF7 breast cancer stem cells (CD44+/CD24-/low): an in vitro study. Drug Des Devel Ther. 2015 Feb 24;9:1193-208. doi: 10.2147/DDDT.S72127
    Species: Human
    Sample type: Cell Lysate
  7. Wu B., Chu X., Feng C., et al. Heat shock protein gp96 decreases p53 stability by regulating Mdm2 E3 ligase activity in liver cancer. Cancer Lett. 2015 Apr 10;359(2):325-34. doi: 10.1016/j.canlet.2015.01.034.
    Species: Human
    Sample type: Cell Lysate
  8. Weng G., Zeng Y., Huang J., et al. Curcumin Enhanced Busulfan-Induced Apoptosis through Downregulating the Expression of Survivin in Leukemia Stem-Like KG1a Cells. Biomed Res Int. 2015;2015:630397. doi: 10.1155/2015/630397.
    Species: Human
    Sample type: Cell Lysate
  9. Knet M., Wartalski K., Hoja-Lukowicz D., et al. Analysis of porcine granulosa cell death signaling pathways induced by vinclozolin. Theriogenology. 2015 Oct 1;84(6):927-39. doi: 10.1016/j.theriogenology.2015.05.028
    Species: Human
    Sample type: Cell Lysate
  10. Leslie K., Whitley G., Herse F., et al. Increased apoptosis, altered oxygen signaling, and antioxidant defenses in first-trimester pregnancies with high-resistance uterine artery blood flow. Am J Pathol. 2015 Oct;185(10):2731-41. doi: 10.1016/j.ajpath.2015.06.020.
    Species: Human
    Sample type: Tissue Lysate
I used this protein array to detect apoptotic proteins in NCM460 epithelial cell lysates. The array worked well and helped me discover a new apoptotic pathway in my project.

UCLA
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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