Human Chemokine Array C1

RayBio® C-Series Human Chemokine Antibody Array 1 Kit. Detects 38 Human Chemokines. Suitable for all liquid sample types.

AAH-CHE-1-2
+

Antigen Information

Gene Symbols:
CCL1 | CCL11 | CCL13 | CCL15 | CCL16 | CCL17 | CCL18 | CCL19 | CCL2 | CCL20   View more

Assay Format

Number of Targets Detected:
38
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
4 or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
BLC
(CXCL13)
CCL28
(MEC)
Ck beta 8-1
(CCL23)
CTACK
(CCL27)
CXCL16
ENA-78
(CXCL5)
Eotaxin-1
(CCL11)
Eotaxin-2
(MPIF-2/CCL24)
Eotaxin-3
(CCL26)
Fractalkine
(CX3CL1)
GCP-2
(CXCL6)
GRO alpha/beta/gamma
GRO alpha
(CXCL1)
HCC-4
(CCL16)
I-309
(TCA-3/CCL1)
I-TAC
(CXCL11)
IL-8
(CXCL8)
IP-10
(CXCL10)
Lymphotactin
(XCL1)
MCP-1
(CCL2)
MCP-2
(CCL8)
MCP-3
(MARC/CCL7)
MCP-4
(CCL13)
MDC
(CCL22)
MIG
(CXCL9)
MIP-1 alpha
(CCL3)
MIP-1 beta
(CCL4)
MIP-1 delta
(CCL15)
MIP-3 alpha
(CCL20)
MIP-3 beta
(CCL19)
MPIF-1
(CCL23)
NAP-2
(PPBP/CXCL7)
PARC
(CCL18)
RANTES
(CCL5)
SDF-1 alpha
(CXCL12 alpha)
SDF-1 beta
(CXCL12 beta)
TARC
(CCL17)
TECK
(CCL25)

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Chemokine Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Zheng Y., Ji S., Wu H., et al. Topical administration of cryopreserved living micronized amnion accelerates wound healing in diabetic mice by modulating local microenvironment. Biomaterials. 2017 Jan;113:56-67. doi: 10.1016/j.biomaterials.2016.10.031.
    Species: Human
    Sample type: Conditioned Media
  2. Roy A., Mondal S., Kordower J., Pahan K. Attenuation of microglial RANTES by NEMO-binding domain peptide inhibits the infiltration of CD8(+) T cells in the nigra of hemiparkinsonian monkey. Neuroscience. 2015 Aug 27;302:36-46. doi: 10.1016/j.neuroscience.2015.03.011.
    Species: Non-Human Primate
    Sample type: Serum
  3. Kumai T., Nagato T., Kobayashi H., et al. CCL17 and CCL22/CCR4 signaling is a strong candidate for novel targeted therapy against nasal natural killer/T-cell lymphoma. Cancer Immunol Immunother. 2015 Jun;64(6):697-705. doi: 10.1007/s00262-015-1675-7
    Species: Human
    Sample type: Conditioned Media
  4. Ryu JH., Park M., Kim B., et al. Tonsil-derived mesenchymal stromal cells produce CXCR2-binding chemokines and acquire follicular dendritic cell-like phenotypes under TLR3 stimulation. Cytokine. 2015 Jun;73(2):225-35. doi: 10.1016/j.cyto.2015.02.028.
    Species: Human
    Sample type: Conditioned Media
  5. Schneberger D., Cloonan D., DeVasure J., et al. Effect of elevated carbon dioxide on bronchial epithelial innate immune receptor response to organic dust from swine confinement barns. Int Immunopharmacol. 2015 Jul;27(1):76-84. doi: 10.1016/j.intimp.2015.04.031.
    Species: Human
    Sample type: Conditioned Media
  6. Wang D., Zou L., Liu X., et al. Chemokine Expression Profiles of Human Hepatoma Cell Lines Mediated by Hepatitis B Virus X Protein. Pathol Oncol Res. 2016 Apr;22(2):393-9. doi: 10.1007/s12253-015-0014-9.
    Species: Human
    Sample type: Cell Lysate
  7. Rizzo M., et al. Low Molecular Weight Hyaluronan-Pulsed Human Dendritic Cells Showed Increased Migration Capacity and Induced Resistance to Tumor Chemoattraction. PLOS One, Published: September 19, 2014. DOI: 10.1371/journal.pone.0107944
    Species: Human
    Sample type: Conditioned Media
  8. De Boeck A., et al. Differential secretome analysis of cancer-associated fibroblasts and bone marrow-derived precursors to identify microenvironmental regulators of colon cancer progression. Proteomics. 2013 Jan;13(2):379-88. doi: 10.1002/pmic.201200179. Epub 2013 Jan 6.
    Species: Human
    Sample type: Conditioned Media
  9. Darbinyan, Armine, et al. "Polyomavirus JC infection inhibits differentiation of oligodendrocyte progenitor cells." Journal of neuroscience research 91.1 (2013): 116-127.
    Species: Human
    Sample type: Conditioned Media
  10. Al-Alwan LA., Chang Y., Baglole C., et al. Autocrine-regulated airway smooth muscle cell migration is dependent on IL-17-induced growth-related oncogenes. J Allergy Clin Immunol. 2012 Oct;130(4):977-85.e6. doi: 10.1016/j.jaci.2012.04.042
    Species: Human
    Sample type: Conditioned Media
The kit I purchased is AAH-CHE-1-4. I used it to detect chemokine from medium. Different expression levels of 5 chemokine were detected. Sample incubation was overnight at 4 degree and the other incubations were 2hrs at RT. Overall, the kit is easy to use.

WAYNE STATE UNIVERSITY
I used this kit 2 years ago, the impression to me is cost-effectiveness and the sensitivity is high enough, although not as high-throughput as the G-series.
This product was used to determine the differences in chemokine secretion by human lung cancer cells expressing different FGFR
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.