Human Chemokine Array C1

RayBio® C-Series Human Chemokine Antibody Array 1 Kit. Detects 38 Human Chemokines. Suitable for all liquid sample types.

$358.55  
AAH-CHE-1-2
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Antigen Information

Gene Symbols:
CCL1 | CCL11 | CCL13 | CCL15 | CCL16 | CCL17 | CCL18 | CCL19 | CCL2 | CCL20   View more

Assay Format

Number of Targets Detected:
38
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
4 or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
BLC
(CXCL13)
CCL28
(MEC)
Ck beta 8-1
(CCL23)
CTACK
(CCL27)
CXCL16
ENA-78
(CXCL5)
Eotaxin-1
(CCL11)
Eotaxin-2
(MPIF-2/CCL24)
Eotaxin-3
(CCL26)
Fractalkine
(CX3CL1)
GCP-2
(CXCL6)
GRO alpha/beta/gamma
GRO alpha
(CXCL1)
HCC-4
(CCL16)
I-309
(TCA-3/CCL1)
I-TAC
(CXCL11)
IL-8
(CXCL8)
IP-10
(CXCL10)
Lymphotactin
(XCL1)
MCP-1
(CCL2)
MCP-2
(CCL8)
MCP-3
(MARC/CCL7)
MCP-4
(CCL13)
MDC
(CCL22)
MIG
(CXCL9)
MIP-1 alpha
(CCL3)
MIP-1 beta
(CCL4)
MIP-1 delta
(CCL15)
MIP-3 alpha
(CCL20)
MIP-3 beta
(CCL19)
MPIF-1
(CCL23)
NAP-2
(PPBP/CXCL7)
PARC
(CCL18)
RANTES
(CCL5)
SDF-1 alpha
(CXCL12 alpha)
SDF-1 beta
(CXCL12 beta)
TARC
(CCL17)
TECK
(CCL25)

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Chemokine Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Jiang S-H., Zhu L-L., Zhang M., et al. GABRP regulates chemokine signalling, macrophage recruitment and tumour progression in pancreatic cancer through tuning KCNN4-mediated Ca2+ signalling in a GABA-independent manner. Gut . 2019 Nov;68(11):1994-2006. doi: 10.1136/gutjnl-2018-317479. Epub 2019 Mar 2.
    Species: Human
    Sample type: Conditioned Media (Pancreatic cancer cells and macrophage infiltration)
  2. Jamieson T, Clarke M, Steele CW, et al. Inhibition of CXCR2 profoundly suppresses inflammation-driven and spontaneous tumorigenesis. The Journal of Clinical Investigation 2012;122(9):3127-3144. doi:10.1172/JCI61067.
    Species: Human
    Sample type: Conditioned Media (Crypt and Adenoma cultures)
  3. Al-Alwan LA., Chang Y., Baglole C., et al. Autocrine-regulated airway smooth muscle cell migration is dependent on IL-17-induced growth-related oncogenes. J Allergy Clin Immunol. 2012 Oct;130(4):977-85.e6. doi: 10.1016/j.jaci.2012.04.042
    Species: Human
    Sample type: Conditioned Media (Airway smooth muscle cells treated with IL-17A and F)
  4. Zheng Y., Ji S., Wu H., et al. Topical administration of cryopreserved living micronized amnion accelerates wound healing in diabetic mice by modulating local microenvironment. Biomaterials. 2017 Jan;113:56-67. doi: 10.1016/j.biomaterials.2016.10.031.
    Species: Human
    Sample type: Conditioned Media (Living Micronized Amniotic Membrane)
  5. Sze S, Kleijn D, Lai R, Tan E, Zhao H,et al.Elucidating the Secretion Proteome of Human Embryonic Stem Cell-derived Mesenchymal Stem Cells.Mol Cell Proteomics. 2007; 6: 1680-1693
    Species: Human
    Sample type: Conditioned Media (conditioned or non-conditioned medium)
  6. Hsu M, Wu S, Chang S, Su I, Tsai C,et al.Epstein-Barr Virus Lytic Transactivator Zta Enhances Chemotactic Activity through Induction of Interleukin-8 in Nasopharyngeal Carcinoma Cells. J. Virol. April 2008 vol. 82 no. 7 3679-3688
    Species: Human
    Sample type: Conditioned Media (Conditioned Media)
  7. McAlpine SM., Issekutz TB., Marshall JS. Virus stimulation of human mast cells results in the recruitment of CD56? T cells by a mechanism dependent on CCR5 ligands. FASEB J. 2012 Mar;26(3):1280-9. doi: 10.1096/fj.11-188979.
    Species: Human
    Sample type: Conditioned Media (Cord-blood derived mast cells)
  8. Durand S H, Flacher V, Romeas A, Carrouel F,Colomb E,et al.Lipoteichoic Acid Increases TLR and Functional Chemokine Expression while Reducing Dentin Formation in In Vitro Differentiated Human Odontoblasts.J Immunol, 2006, 176: 2880-2887
    Species: Human
    Sample type: Conditioned Media (supernatants recovered from odontoblasts cultured)
  9. Farahi N,Cowburn A S,Upton P D, Deighton J, Sobolewski A,et al.Eotaxin-1/CC Chemokine Ligand 11: A Novel Eosinophil Survival Factor Secreted by Human Pulmonary Artery Endothelial Cells. J Immunol. 2007;179:1264-1273
    Species: Human
    Sample type: Conditioned Media (HUVEC and HPAEC media)
  10. McGovern NN, Cowburn AS, Porter L, Walmsley SR, Thompson AAR, et al. Hypoxia Selectively Inhibits Respiratory Burst Activity and Killing of Staphylococcus aureus in Human Neutrophils. J Immunol. 2011;186:453-463.
    Species: Human
    Sample type: Conditioned Media (Neutrophil supernatants)
The kit I purchased is AAH-CHE-1-4. I used it to detect chemokine from medium. Different expression levels of 5 chemokine were detected. Sample incubation was overnight at 4 degree and the other incubations were 2hrs at RT. Overall, the kit is easy to use.

WAYNE STATE UNIVERSITY
I used this kit 2 years ago, the impression to me is cost-effectiveness and the sensitivity is high enough, although not as high-throughput as the G-series.
This product was used to determine the differences in chemokine secretion by human lung cancer cells expressing different FGFR
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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