Human Chemokine Array C1

RayBio® C-Series Human Chemokine Antibody Array 1 Kit. Detects 38 Human Chemokines. Suitable for all liquid sample types.

$358.55  
AAH-CHE-1-2
+

Antigen Information

Gene Symbols:
CCL1 | CCL11 | CCL13 | CCL15 | CCL16 | CCL17 | CCL18 | CCL19 | CCL2 | CCL20   View more

Assay Format

Number of Targets Detected:
38
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
4 or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
BLC
(CXCL13)
CCL28
(MEC)
Ck beta 8-1
(CCL23)
CTACK
(CCL27)
CXCL16
ENA-78
(CXCL5)
Eotaxin-1
(CCL11)
Eotaxin-2
(MPIF-2/CCL24)
Eotaxin-3
(CCL26)
Fractalkine
(CX3CL1)
GCP-2
(CXCL6)
GRO alpha/beta/gamma
GRO alpha
(CXCL1)
HCC-4
(CCL16)
I-309
(TCA-3/CCL1)
I-TAC
(CXCL11)
IL-8
(CXCL8)
IP-10
(CXCL10)
Lymphotactin
(XCL1)
MCP-1
(CCL2)
MCP-2
(CCL8)
MCP-3
(MARC/CCL7)
MCP-4
(CCL13)
MDC
(CCL22)
MIG
(CXCL9)
MIP-1 alpha
(CCL3)
MIP-1 beta
(CCL4)
MIP-1 delta
(CCL15)
MIP-3 alpha
(CCL20)
MIP-3 beta
(CCL19)
MPIF-1
(CCL23)
NAP-2
(PPBP/CXCL7)
PARC
(CCL18)
RANTES
(CCL5)
SDF-1 alpha
(CXCL12 alpha)
SDF-1 beta
(CXCL12 beta)
TARC
(CCL17)
TECK
(CCL25)

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Chemokine Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
The kit I purchased is AAH-CHE-1-4. I used it to detect chemokine from medium. Different expression levels of 5 chemokine were detected. Sample incubation was overnight at 4 degree and the other incubations were 2hrs at RT. Overall, the kit is easy to use.

WAYNE STATE UNIVERSITY
I used this kit 2 years ago, the impression to me is cost-effectiveness and the sensitivity is high enough, although not as high-throughput as the G-series.
This product was used to determine the differences in chemokine secretion by human lung cancer cells expressing different FGFR
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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