Human Chemokine Array C1

RayBio® C-Series Human Chemokine Antibody Array 1 Kit. Detects 38 Human Chemokines. Suitable for all liquid sample types.


Assay Format

Number of Targets Detected:
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
  • Semi-Quantitative
Solid Support:

Product Specifications

4 or 8 Sample Kit

Protein Information

Gene Symbols:
CCL1 | CCL11 | CCL13 | CCL15 | CCL16 | CCL17 | CCL18 | CCL19 | CCL2 | CCL20   View more

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Ck beta 8-1
GRO alpha/beta/gamma
GRO alpha
MIP-1 alpha
MIP-1 beta
MIP-1 delta
MIP-3 alpha
MIP-3 beta
SDF-1 alpha
(CXCL12 alpha)
SDF-1 beta
(CXCL12 beta)

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Chemokine Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis


For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Roy A., Mondal S., Kordower J., Pahan K. Attenuation of microglial RANTES by NEMO-binding domain peptide inhibits the infiltration of CD8(+) T cells in the nigra of hemiparkinsonian monkey. Neuroscience. 2015 Aug 27;302:36-46. doi: 10.1016/j.neuroscience.2015.03.011.
    Species: Non-Human Primate
    Sample type: Serum (Rhesus monkeys with hemiparkinson's)
  2. Ryu JH., Park M., Kim B., et al. Tonsil-derived mesenchymal stromal cells produce CXCR2-binding chemokines and acquire follicular dendritic cell-like phenotypes under TLR3 stimulation. Cytokine. 2015 Jun;73(2):225-35. doi: 10.1016/j.cyto.2015.02.028.
    Species: Human
    Sample type: Conditioned Media (TLR3 stimulated tonsilar mononuclaear stromal cells)
  3. Brown MG, McAlpine SM, Huang YY, et al. RNA Sensors Enable Human Mast Cell Anti-Viral Chemokine Production and IFN-Mediated Protection in Response to Antibody-Enhanced Dengue Virus Infection. Li K, ed. PLoS ONE 2012;7(3):e34055. doi:10.1371/journal.pone.0034055.
    Species: Human
    Sample type: Conditioned Media (Cord blodd derived mast cells (CBMCs))
  4. Rizzo M., et al. Low Molecular Weight Hyaluronan-Pulsed Human Dendritic Cells Showed Increased Migration Capacity and Induced Resistance to Tumor Chemoattraction. PLOS One, Published: September 19, 2014. DOI: 10.1371/journal.pone.0107944
    Species: Human
    Sample type: Conditioned Media (Colorectal cancer patients)
  5. Tomita R, Sasabe E, Tomomura A, Yamamoto T. Macrophage?derived exosomes attenuate the susceptibility of oral squamous cell carcinoma cells to chemotherapeutic drugs through the AKT/GSK?3? pathway. Oncol Rep. 2020 Nov;44(5):1905-1916. doi: 10.3892/or.2020.7748. Epub 2020 Sep 2. PMID: 32901850; PMCID: PMC7551207.
    Species: Human
    Sample type: Cell Lysate (Exosomes lysates, OSC-4 cells, tongue cancer)
  6. Wang D., Zou L., Liu X., et al. Chemokine Expression Profiles of Human Hepatoma Cell Lines Mediated by Hepatitis B Virus X Protein. Pathol Oncol Res. 2016 Apr;22(2):393-9. doi: 10.1007/s12253-015-0014-9.
    Species: Human
    Sample type: Cell Lysate (Human hepatoma cell lines)
  7. Behjati S., Basu B., Wallace R., et al. STAT3 Regulates Proliferation and Immunogenicity of the Ewing Family of Tumors In Vitro. Sarcoma. 2012; 2012: 987239. doi: 10.1155/2012/987239
    Species: Human
    Sample type: Conditioned Media (Stat3 regulation in Ewing sarcoma Family Tumor model)
Used for tissue lysate differential. Chemokine signals weak although blot controls appeared strong.

N Central
The kit I purchased is AAH-CHE-1-4. I used it to detect chemokine from medium. Different expression levels of 5 chemokine were detected. Sample incubation was overnight at 4 degree and the other incubations were 2hrs at RT. Overall, the kit is easy to use.

I used this kit 2 years ago, the impression to me is cost-effectiveness and the sensitivity is high enough, although not as high-throughput as the G-series.
This product was used to determine the differences in chemokine secretion by human lung cancer cells expressing different FGFR
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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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