Human Cytokine Array C3

RayBio® C-Series Human Cytokine Antibody Array 3 Kit. Detects 42 Humans Cytokines. Suitable for all liquid sample types.


Antigen Information

Gene Symbols:
ANG | CCL1 | CCL15 | CCL17 | CCL2 | CCL22 | CCL5 | CCL7 | CCL8 | CSF1   View more

Assay Format

Number of Targets Detected:
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Solid Support:

Product Specifications

4 or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
GRO alpha/beta/gamma
GRO alpha
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-12 p40/p70
  • Gene Symbol: IL12A; IL12B
  • Accession #: P29459
MIP-1 delta
SDF-1 alpha
(CXCL12 alpha)
TGF beta 1
TNF alpha
TNF beta
Oncostatin M

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Cytokine Antibody Array C3 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis


For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Ibrahim S., Gadalla R., El-Ghonaimy E., et al. Syndecan-1 is a novel molecular marker for triple negative inflammatory breast cancer and modulates the cancer stem cell phenotype via the IL-6/STAT3, Notch and EGFR signaling pathways. Mol Cancer. 2017 Mar 7;16(1):57. doi: 10.1186/s12943-017-0621-z.
    Species: Human
    Sample type: Conditioned Media
  2. Kim, Seog?Young, et al. "Cancer Stem Cells Protect Non?Stem Cells from Anoikis: Bystander Effects." Journal of cellular biochemistry (2016).
    Species: Human
    Sample type: Conditioned Media
  3. Kim MK., Seo BF., Kim KJ., et al. Secretory factors of human chorion-derived stem cells enhance activation of human fibroblasts. Cytotherapy. 2015 Mar;17(3):301-9. doi: 10.1016/j.jcyt.2014.10.007.
    Species: Human
    Sample type: Conditioned Media
  4. Moore Q., Smith C., Wolf S., et al. Examining the immune response of pneumolysin exposed human corneal epithelial cells in a keratitis model. BIOS 85(4):199-206. 2014. doi:
    Species: Human
    Sample type: Conditioned Media
  5. Zhang L., Zhang S., Yao J., et al. Microenvironment-induced PTEN loss by exosomal microRNA primes brain metastasis outgrowth. Nature. 2015 Oct 19. doi: 10.1038/nature15376.
    Species: Human
    Sample type: Conditioned Media
  6. Renn T, Kao Y., Wang C., Burnouf T. Anti-inflammatory effects of platelet biomaterials in a macrophage cellular model. Vox Sang. 2015 Aug;109(2):138-47. doi: 10.1111/vox.12264.
    Species: Human
    Sample type: Platelet Releasate
  7. Ito Y., Ishiguro H., Kobayashi N., et al. Adipocyte-derived monocyte chemotactic protein-1 (MCP-1) promotes prostate cancer progression through the induction of MMP-2 activity. Prostate. 2015 Jul 1;75(10):1009-19. doi: 10.1002/pros.22972.
    Species: Human
    Sample type: Conditioned Media
  8. Kepler C., Markova D., Koerner J., et al. Substance P Receptor Antagonist Suppresses Inflammatory Cytokine Expression in Human Disc Cells. Spine (Phila Pa 1976). 2015 Aug 15;40(16):1261-9. doi: 10.1097/BRS.0000000000000954.
    Species: Human
    Sample type: Conditioned Media
  9. Lindholm P., Sivapurapu N., Jovanovic B., et al. Monocyte-Induced Prostate Cancer Cell Invasion is Mediated by Chemokine ligand 2 and Nuclear Factor-?B Activity. J Clin Cell Immunol 2015, 6:2.
    Species: Human
    Sample type: Conditioned Media
  10. Yang C., Chiba T., Brill B., et al. Expression of the miR-302/367 cluster in glioblastoma cells suppresses tumorigenic gene expression patterns and abolishes transformation related phenotypes. Int J Cancer. 2015 Nov 15;137(10):2296-309. doi: 10.1002/ijc.29606
    Species: Human
    Sample type: Conditioned Media
The array is very easy to follow. It comes with very through instruction and pictures. I have been away from experiments for several years. Being back to lab after those years, this very detailed and highly organized assay made my life so much easier. Wish every test package comes like that

University of the Pacific
Easy to use and reliable

Dana Farber Cancer Institute
This was exceptionally easy to run and image
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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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