Human Cytokine Array C3

RayBio® C-Series Human Cytokine Antibody Array 3 Kit. Detects 42 Humans Cytokines. Suitable for all liquid sample types.


Antigen Information

Gene Symbols:
ANG | CCL1 | CCL15 | CCL17 | CCL2 | CCL22 | CCL5 | CCL7 | CCL8 | CSF1   View more

Assay Format

Number of Targets Detected:
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Solid Support:

Product Specifications

4 or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
GRO alpha/beta/gamma
GRO alpha
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-12 p40/p70
  • Gene Symbol: IL12A; IL12B
  • Accession #: P29459
MIP-1 delta
SDF-1 alpha
(CXCL12 alpha)
TGF beta 1
TNF alpha
TNF beta
Oncostatin M

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Cytokine Antibody Array C3 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis


For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Lee S., Kim K., Min K., Kim K., Chang S., Kim J. Angiogenin Reduces Immune Inflammation via inhibition of TANK-Binding Kinase 1 Expression in Human Corneal Fibroblast Cells. Mediators of InflammationVolume 2014 (2014), Article ID 861435, 12 pages
    Species: Human
    Sample type: Conditioned Media
  2. Li Y., et al. Myokine IL-15 regulates the crosstalk of co-cultured porcineskeletal muscle satellite cells and preadipocytes. Mol Biol Rep 2014, Date: 07 Aug 2014. DOI 10.1007/s11033-014-3646-z
    Species: Porcine
    Sample type: Conditioned Media
  3. McNicholas et al.: Cytokine responses toStaphylococcus aureus bloodstream infection differ between patientcohorts that have different clinical courses of infection. BMC InfectiousDiseases 2014 14:580.
    Species: Human
    Sample type: Plasma
  4. Riverso M., Kortenkamp A., Silva E., Non-tumorigenic epithelial cells secrete MCP-1 and other cytokines that promote cell division in breast cancer cells by activating ER? via PI3 K/Akt/mTOR signaling. The International Journal of Biochemistry & Cell Biology 2014, June Epub Ahead of Print.
    Species: Human
    Sample type: Conditioned Media
  5. Shanmugam A., Wang J., Markand S., et al. Sigma receptor 1 activation attenuates release of inflammatory cytokines MIP1?, MIP2, MIP3?, and IL12 (p40/p70) by retinal Müller glial cells. J Neurochem. 2014 Dec 1. doi: 10.1111/jnc.13002
    Species: Mouse
    Sample type: Conditioned Media
  6. Kim SY., Kang JW., Song X., et al. Role of the IL-6-JAK1-STAT3-Oct-4 pathway in the conversion of non-stem cancer cells into cancer stem-like cells
    Species: Human
    Sample type: Conditioned Media
  7. Kesanakurti, Divya, et al. "Role of MMP-2 in the regulation of IL-6/Stat3 survival signaling via interaction with ?5?1 integrin in glioma." Oncogene 32.3 (2013): 327-340.
    Species: Human
    Sample type: Conditioned Media
  8. Gest, Caroline, et al. "Rac3 induces a molecular pathway triggering breast cancer cell aggressiveness: differences in MDA-MB-231 and MCF-7 breast cancer cell lines." BMC cancer 13.1 (2013): 63.
    Species: Human
    Sample type: Conditioned Media
  9. Kepler, Christopher K., et al. "Expression and relationship of proinflammatory chemokine RANTES/CCL5 and cytokine IL-1? in painful human intervertebral discs." Spine 38.11 (2013): 873-880.
    Species: Human
    Sample type: Tissue Lysate
  10. Ergun, M., et al. "Effects of exercise on angiogenesis and apoptosis?related molecules, quality of life, fatigue and depression in breast cancer patients." European journal of cancer care 22.5 (2013): 626-637.
    Species: Human
    Sample type: Serum
The array is very easy to follow. It comes with very through instruction and pictures. I have been away from experiments for several years. Being back to lab after those years, this very detailed and highly organized assay made my life so much easier. Wish every test package comes like that

University of the Pacific
Easy to use and reliable

Dana Farber Cancer Institute
This was exceptionally easy to run and image
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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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