Human Cytokine Array C3

RayBio® C-Series Human Cytokine Antibody Array 3 Kit. Detects 42 Humans Cytokines. Suitable for all liquid sample types.


Assay Format

Number of Targets Detected:
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
  • Semi-Quantitative
Solid Support:

Product Specifications

4 or 8 Sample Kit

Protein Information

Gene Symbols:
ANG | CCL1 | CCL15 | CCL17 | CCL2 | CCL22 | CCL5 | CCL7 | CCL8 | CSF1   View more

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
GRO alpha/beta/gamma
GRO alpha
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-12 p40/p70
  • Gene Symbol: IL12A; IL12B
  • Accession #: P29459
MIP-1 delta
SDF-1 alpha
(CXCL12 alpha)
TGF beta 1
TNF alpha
TNF beta
Oncostatin M

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Cytokine Antibody Array C3 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis


For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Scuto, Anna, et al. "STAT3 inhibition is a therapeutic strategy for ABC-like diffuse large B-cell lymphoma." Cancer research 71.9 (2011): 3182-3188.
    Species: Human
    Sample type: Conditioned Media
  2. Cho, Yoon, et al. "Identification of novel cytokine biomarkers of hexanal exposure associated with pulmonary toxicity." Environmental Pollution 229 (2017): 810-817.
    Species: Human
    Sample type: Cell Lysate (lung cells)
  3. Kesanakurti, Divya, et al. "Role of MMP-2 in the regulation of IL-6/Stat3 survival signaling via interaction with ?5?1 integrin in glioma." Oncogene 32.3 (2013): 327-340.
    Species: Human
    Sample type: Conditioned Media (pSV- and pM-treated cells)
  4. Kverka M, Burianova J, Lodinova-Zadnikova R, Kocuirkova I, et al. Cytokine Profiling in Human Colostrum and Milk by Protein Array. Clin Chem. 2007; 53(5):955-962.
    Species: Human
    Sample type: Milk/Colostrum (Human milk and colostrum)
  5. Li et al.: TWIST1 associates with NF- B subunit RELAvia carboxyl-terminal WR domain to promote cell autonomous invasionthrough IL8 production. BMC Biology 2012 10:73.
    Species: Human
    Sample type: Conditioned Media (MCF-10A cells)
  6. Gest C., Mirshahi P., Pritchard L., et al. Ovarian cancer: Stat3, RhoA and IGF-IR as therapeutic targets. Cancer Lett. 2012 Apr 28;317(2):207-17. doi: 10.1016/j.canlet.2011.11.026
    Species: Human
    Sample type: Conditioned Media (Ovarian Cancer Cells)
  7. Formentini, Laura, et al. "Mitochondrial H+-ATP synthase in human skeletal muscle: contribution to dyslipidaemia and insulin resistance." Diabetologia 60.10 (2017): 2052-2065.
    Species: Human
    Sample type: Tissue Lysate (skeletal muscle)
  8. Wanichkul T., Han S., Huang RP., Sidell N. Cytokine regulation by peroxisome proliferator-activated receptor gamma in human endometrial cells. Fertil Steril. 2003 Mar;79 Suppl 1:763-9.
    Species: Human
    Sample type: Conditioned Media (Endometrial Cell conditioned media)
  9. Braun, Jennifer, et al. "Endothelial cell ephrinB2-dependent activation of monocytes in arteriosclerosis." Arteriosclerosis, thrombosis, and vascular biology 31.2 (2011): 297-305.
    Species: Human
    Sample type: Conditioned Media
  10. Furukawa, Kanako, et al. "Increase of nitrosative stress in patients with eosinophilic pneumonia." Respiratory research 12.1 (2011): 81.
    Species: Human
    Sample type: Exhaled Breath Condensate
The array is very easy to follow. It comes with very through instruction and pictures. I have been away from experiments for several years. Being back to lab after those years, this very detailed and highly organized assay made my life so much easier. Wish every test package comes like that

University of the Pacific
Easy to use and reliable

Dana Farber Cancer Institute
This was exceptionally easy to run and image
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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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