Human Cytokine Array C3

RayBio® C-Series Human Cytokine Antibody Array 3 Kit. Detects 42 Humans Cytokines. Suitable for all liquid sample types.


Assay Format

Number of Targets Detected:
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
  • Semi-Quantitative
Solid Support:

Product Specifications

4 or 8 Sample Kit

Protein Information

Gene Symbols:
ANG | CCL1 | CCL15 | CCL17 | CCL2 | CCL22 | CCL5 | CCL7 | CCL8 | CSF1   View more

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
GRO alpha/beta/gamma
GRO alpha
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-12 p40/p70
  • Gene Symbol: IL12A; IL12B
  • Accession #: P29459
MIP-1 delta
SDF-1 alpha
(CXCL12 alpha)
TGF beta 1
TNF alpha
TNF beta
Oncostatin M

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Cytokine Antibody Array C3 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis


For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Venkatesh A, Nandigam H, Muccioli M, Singh M, Loftus T, Lewis D, Pate M, Benencia F. Regulation of inflammatory factors by double-stranded RNA receptors in breast cancer cells. Immunobiology. 2018 Jun-Jul;223(6-7):466-476. doi: 10.1016/j.imbio.2017.11.005. Epub 2017 Nov 22. PMID: 29331323.
    Species: Human
    Sample type: Conditioned Media
  2. Craigie M, Cicalese S, Sariyer IK. Neuroimmune Regulation of JC Virus by Intracellular and Extracellular Agnoprotein. J Neuroimmune Pharmacol. 2018 Jun;13(2):126-142. doi: 10.1007/s11481-017-9770-5. Epub 2017 Nov 20. PMID: 29159704; PMCID: PMC5930035.roimmune Regulation of JC Virus by Intracellular and Extracellular Agnoprotein
    Species: Human
    Sample type: Conditioned media
  3. Formentini, Laura, et al. "Mitochondrial H+-ATP synthase in human skeletal muscle: contribution to dyslipidaemia and insulin resistance." Diabetologia 60.10 (2017): 2052-2065.
    Species: Human
    Sample type: Tissue Lysate
  4. Cho, Yoon, et al. "Identification of novel cytokine biomarkers of hexanal exposure associated with pulmonary toxicity." Environmental Pollution 229 (2017): 810-817.
    Species: Human
    Sample type: Cell Lysate
  5. Kim, Seog?Young, et al. "Cancer Stem Cells Protect Non?Stem Cells from Anoikis: Bystander Effects." Journal of cellular biochemistry (2016).
    Species: Human
    Sample type: Conditioned Media
  6. Kim MK., Seo BF., Kim KJ., et al. Secretory factors of human chorion-derived stem cells enhance activation of human fibroblasts. Cytotherapy. 2015 Mar;17(3):301-9. doi: 10.1016/j.jcyt.2014.10.007.
    Species: Human
    Sample type: Conditioned Media
  7. Moore Q., Smith C., Wolf S., et al. Examining the immune response of pneumolysin exposed human corneal epithelial cells in a keratitis model. BIOS 85(4):199-206. 2014. doi:
    Species: Human
    Sample type: Conditioned Media
  8. Zhang L., Zhang S., Yao J., et al. Microenvironment-induced PTEN loss by exosomal microRNA primes brain metastasis outgrowth. Nature. 2015 Oct 19. doi: 10.1038/nature15376.
    Species: Human
    Sample type: Conditioned Media
  9. Renn T, Kao Y., Wang C., Burnouf T. Anti-inflammatory effects of platelet biomaterials in a macrophage cellular model. Vox Sang. 2015 Aug;109(2):138-47. doi: 10.1111/vox.12264.
    Species: Human
    Sample type: Platelet Releasate
  10. Ito Y., Ishiguro H., Kobayashi N., et al. Adipocyte-derived monocyte chemotactic protein-1 (MCP-1) promotes prostate cancer progression through the induction of MMP-2 activity. Prostate. 2015 Jul 1;75(10):1009-19. doi: 10.1002/pros.22972.
    Species: Human
    Sample type: Conditioned Media
The array is very easy to follow. It comes with very through instruction and pictures. I have been away from experiments for several years. Being back to lab after those years, this very detailed and highly organized assay made my life so much easier. Wish every test package comes like that

University of the Pacific
Easy to use and reliable

Dana Farber Cancer Institute
This was exceptionally easy to run and image
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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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