Human Cytokine Array C3

RayBio® C-Series Human Cytokine Antibody Array 3 Kit. Detects 42 Humans Cytokines. Suitable for all liquid sample types.

$440.36  
AAH-CYT-3-2
+

Antigen Information

Gene Symbols:
ANG | CCL1 | CCL15 | CCL17 | CCL2 | CCL22 | CCL5 | CCL7 | CCL8 | CSF1   View more

Assay Format

Number of Targets Detected:
42
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
4 or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
ENA-78
(CXCL5)
GCSF
GM-CSF
GRO alpha/beta/gamma
GRO alpha
(CXCL1)
I-309
(TCA-3/CCL1)
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-2
IL-3
IL-4
IL-5
IL-6
IL-7
IL-8
(CXCL8)
IL-10
IL-12 p40/p70
  • Gene Symbol: IL12A; IL12B
  • Accession #: P29459
IL-13
IL-15
IFN-gamma
MCP-1
(CCL2)
MCP-2
(CCL8)
MCP-3
(MARC/CCL7)
M-CSF
MDC
(CCL22)
MIG
(CXCL9)
MIP-1 delta
(CCL15)
RANTES
(CCL5)
SCF
SDF-1 alpha
(CXCL12 alpha)
TARC
(CCL17)
TGF beta 1
TNF alpha
TNF beta
(TNFSF1B)
EGF
IGF-1
Angiogenin
Oncostatin M
Thrombopoietin
(TPO)
VEGF-A
PDGF-BB
Leptin

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Cytokine Antibody Array C3 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Marks DJB, Harbord MWN, MacAllister R, Rahman FZ, Young J, et al. Defective acute inflammation in Crohn's disease: a clinical investigation. Lancet. 2006;367:668-678.
    Species: Human
    Sample type: Tissue Lysate (Supernates of forearm epidermal "skin windows")
  2. Zhang L., Zhang S., Yao J., et al. Microenvironment-induced PTEN loss by exosomal microRNA primes brain metastasis outgrowth. Nature. 2015 Oct 19. doi: 10.1038/nature15376.
    Species: Human
    Sample type: Conditioned Media (231 MDA cancer cell lines)
  3. Nick JA, Coldren CD, Geraci MA, Poch KR, et al. Recombinant human activated protein C reduces human endotoxin-inducedpulmonary inflammation via inhibition of neutrophil chemotaxis. Blood. 2004;104(13):3878-3885.
    Species: Human
    Sample type: BAL Fluid (Bronchioalveolar lavage fluid (BALF))
  4. Réthi B, Gogolák P, Szatmari I, Veres A, et al. SLAM/SLAM interactions inhibit CD40-induced production of inflammatorycytokines in monocyte-derived dendritic cells. Blood. 2006;107:2821-2829.
    Species: Human
    Sample type: Conditioned Media (Cell culture supernates from dendritic cells)
  5. Wang S, Yang J, Qian J, Wezeman M, Kwak LW, Yi Q. Tumor evasion of the immune system: inhibiting p38 MAPK signaling restores the function of dendritic cells in multiple myeloma. Blood. 2006;107:2431-2439.
    Species: Human
    Sample type: Conditioned Media (CD4+ T lymphocytes)
  6. Coppinger JA, O'Connor R, Wynne K, Flanagan M, Sullivan M, et al. Moderation of the platelet releasate response by aspirin. Blood. 2007;109:4786-4792.
    Species: Human
    Sample type: Platelet Releasate (Platelet aggregate supernatant (releasate))
  7. Gogolak P, Rethi B, Szatmari I, Lanyi A, et al. Differentiation of CD1a- and CD1a+ monocyte-derived dendritic cells is biased by lipid environment and PPAR-gamma. Blood. 2007; 109:643-652.
    Species: Human
    Sample type: Conditioned Media (Monocyte-derived dendritic cell-cultured medium)
  8. Morandi, Fabio, et al. "Soluble HLA-G dampens CD94/NKG2A expression and function and differentially modulates chemotaxis and cytokine and chemokine secretion in CD56bright and CD56dim NK cells." Blood 118.22 (2011): 5840-5850.
    Species: Human
    Sample type: Conditioned Media (Nk and PB cells)
  9. Ibrahim S., Gadalla R., El-Ghonaimy E., et al. Syndecan-1 is a novel molecular marker for triple negative inflammatory breast cancer and modulates the cancer stem cell phenotype via the IL-6/STAT3, Notch and EGFR signaling pathways. Mol Cancer. 2017 Mar 7;16(1):57. doi: 10.1186/s12943-017-0621-z.
    Species: Human
    Sample type: Conditioned Media (Sum49 and inflammatory breast cancer cell line.)
  10. Kerur, Nagaraj, et al. "IFI16 acts as a nuclear pathogen sensor to induce the inflammasome in response to Kaposi Sarcoma-associated herpesvirus infection." Cell host & microbe 9.5 (2011): 363-375.
    Species: Human
    Sample type: Conditioned Media
The array is very easy to follow. It comes with very through instruction and pictures. I have been away from experiments for several years. Being back to lab after those years, this very detailed and highly organized assay made my life so much easier. Wish every test package comes like that

University of the Pacific
Easy to use and reliable

Dana Farber Cancer Institute
This was exceptionally easy to run and image
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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