Human Cytokine Array C3

RayBio® C-Series Human Cytokine Antibody Array 3 Kit. Detects 42 Humans Cytokines. Suitable for all liquid sample types.

$440.36  
AAH-CYT-3-2
+

Antigen Information

Gene Symbols:
ANG | CCL1 | CCL15 | CCL17 | CCL2 | CCL22 | CCL5 | CCL7 | CCL8 | CSF1   View more

Assay Format

Number of Targets Detected:
42
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
4 or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
ENA-78
(CXCL5)
GCSF
GM-CSF
GRO alpha/beta/gamma
GRO alpha
(CXCL1)
I-309
(TCA-3/CCL1)
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-2
IL-3
IL-4
IL-5
IL-6
IL-7
IL-8
(CXCL8)
IL-10
IL-12 p40/p70
  • Gene Symbol: IL12A; IL12B
  • Accession #: P29459
IL-13
IL-15
IFN-gamma
MCP-1
(CCL2)
MCP-2
(CCL8)
MCP-3
(MARC/CCL7)
M-CSF
MDC
(CCL22)
MIG
(CXCL9)
MIP-1 delta
(CCL15)
RANTES
(CCL5)
SCF
SDF-1 alpha
(CXCL12 alpha)
TARC
(CCL17)
TGF beta 1
TNF alpha
TNF beta
(TNFSF1B)
EGF
IGF-1
Angiogenin
Oncostatin M
Thrombopoietin
(TPO)
VEGF-A
PDGF-BB
Leptin

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Cytokine Antibody Array C3 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Liu C., Choi M., Li X., et al. Immunomodulatory effect of structurally-characterized mushroom sclerotial polysaccharides isolated from Polyporus rhinocerus on human monoctyes THP-1. Journal of Functional Foods Volume 41, February 2018, Pages 90-99. doi.org/10.1016/j.jff.2017.12.039
    Species: Human
    Sample type: Conditioned Media
  2. Gaceb, Abderahim, et al. "Pericytes secrete pro-regenerative molecules in response to platelet-derived growth factor-BB."?Journal of Cerebral Blood Flow & Metabolism?38.1 (2018): 45-57.
    Species: Human
    Sample type: cell supernatants
  3. Ibrahim S., Gadalla R., El-Ghonaimy E., et al. Syndecan-1 is a novel molecular marker for triple negative inflammatory breast cancer and modulates the cancer stem cell phenotype via the IL-6/STAT3, Notch and EGFR signaling pathways. Mol Cancer. 2017 Mar 7;16(1):57. doi: 10.1186/s12943-017-0621-z.
    Species: Human
    Sample type: Conditioned Media
  4. Formentini, Laura, et al. "Mitochondrial H+-ATP synthase in human skeletal muscle: contribution to dyslipidaemia and insulin resistance."?Diabetologia?60.10 (2017): 2052-2065.
    Species: Human
    Sample type: Tissue Cells
  5. Cho, Yoon, et al. "Identification of novel cytokine biomarkers of hexanal exposure associated with pulmonary toxicity."?Environmental Pollution?229 (2017): 810-817.
    Species: Human
    Sample type: Cell Lysates
  6. Kim, Seog?Young, et al. "Cancer Stem Cells Protect Non?Stem Cells from Anoikis: Bystander Effects."?Journal of cellular biochemistry?(2016).
    Species: Human
    Sample type: Conditioned Media
  7. Kim MK., Seo BF., Kim KJ., et al. Secretory factors of human chorion-derived stem cells enhance activation of human fibroblasts. Cytotherapy. 2015 Mar;17(3):301-9. doi: 10.1016/j.jcyt.2014.10.007.
    Species: Human
    Sample type: Conditioned Media
  8. Zhang L., Zhang S., Yao J., et al. Microenvironment-induced PTEN loss by exosomal microRNA primes brain metastasis outgrowth. Nature. 2015 Oct 19. doi: 10.1038/nature15376.
    Species: Human
    Sample type: Conditioned Media
  9. Renn T, Kao Y., Wang C., Burnouf T. Anti-inflammatory effects of platelet biomaterials in a macrophage cellular model. Vox Sang. 2015 Aug;109(2):138-47. doi: 10.1111/vox.12264.
    Species: Human
    Sample type: Platelet Releasate
  10. Ito Y., Ishiguro H., Kobayashi N., et al. Adipocyte-derived monocyte chemotactic protein-1 (MCP-1) promotes prostate cancer progression through the induction of MMP-2 activity. Prostate. 2015 Jul 1;75(10):1009-19. doi: 10.1002/pros.22972.
    Species: Human
    Sample type: Conditioned Media
The array is very easy to follow. It comes with very through instruction and pictures. I have been away from experiments for several years. Being back to lab after those years, this very detailed and highly organized assay made my life so much easier. Wish every test package comes like that

University of the Pacific
Easy to use and reliable

Dana Farber Cancer Institute
This was exceptionally easy to run and image
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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