Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Human Cytokine Antibody Array C5 Membranes
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Detection Buffer C
Detection Buffer D
8-Well Incubation Tray
Other Materials Required
Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
I used on mammalian cell culture supernatant it and works well
Vaishnavi Indiana University01/07/2021, 02:46 PM
I wanted to get something that can work on primary cell line and it turned out good. Results received after experiments had let us plan on which cytokine study we might need to work on for further study. Kit is simple and was of good quality.
Mubeen University of Texas Health Science Center San Antonio03/02/2020, 10:32 AM
Easy to use, works well.
Erick East Tennessee State University01/17/2020, 04:52 PM
Easy to use, and protocols are easy to follow. Recommended to fellow colleagues.
Raheel UCSF12/03/2019, 03:19 AM
Protocol is simple and worked great with our conditioned media!
S Chaudhary UNMC07/18/2019, 11:30 PM
Simple and easy protocol with clean results.
Manali Augusta University05/03/2019, 10:17 AM
The protocol suggested in the kit works very well for all arrays. Even if you need to optimize the time using the short exposition time suggested in the protocol, your array development will work great as usual!
Thaiz Augusta University12/11/2018, 01:34 PM
Worked great with conditioned media. Followed the protocol in the kit and the results were super clean and of excellent quality.
Suchitra Stanford University11/13/2018, 04:39 PM
it's an easy kit to use. However, I was not very impressed with their excel software. there was a mistake which we caught and ask them to fix bit they didn't even give us a discount. Other than it's straightforward and give you great results.
Harmony University of the pacific09/13/2018, 11:37 AM
Worked great for med-high abundant proteins. Customer service was spectacular
P V raj UCLA07/20/2018, 07:56 PM
works good with condition media
Himanshu Arora04/26/2018, 05:16 PM
Human Cytokine Array C5 kit is simple to use, has high quality and results are reproducible. Fast delivery from Raybiotech. But the value for the money is a little high.